Event-related brain potentials in lexical processing with Chinese characters show effects of contextual diversity but not word frequency.

The diversity of contexts in which a word occurs, operationalized as CD, is strongly correlated with response times in visual word recognition, with higher CD words being recognized faster. CD and token word frequency (WF) are highly correlated but in behavioral studies when other variables that affect word visual recognition are controlled for, the WF effect is eliminated when contextual diversity (CD) is controlled. In contrast, the only event-related potential (ERP) study to examine CD and WF Vergara-Martínez et al.

Effect and safety evaluation of tacrolimus and tripterygium glycosides combined therapy in treatment of Henoch-Schönlein purpura nephritis.

Objective: Henoch-Schönlein purpura nephritis has become a significant threat to children's health. Traditional combined therapy of glucocorticoids and cyclophosphamide leads to severe toxicity and complications. Therefore, identifying a feasible and effective strategy with low side-effects for the treatment of Henoch-Schönlein purpura nephritis is of great significance.

The significance of the hemalexin C1q, RBP, and urinary NAG levels in the diagnosis and prognosis of children with purpura nephritis.

Purpose: To investigate the significance of the hemalexin C1q, retinal-binding-protein (RBP), and urinary N-acety1-β-D-glucosaminidase (NAG) levels in the diagnosis and prognosis of children with purpura nephritis.

Methods: A total of 130 children with purpura nephritis admitted to our hospital from January 2017 to December 2019 were recruited as the study cohort, including 43 children with purpura nephritis as the observation group, 51 children with purpura nephritis as the control group, and 36 healthy children undergoing physical examinations at the same time period as the healthy group. The basic data of the three groups of children were compared, and the hemalexin C1q, RBP, and urinary NAG levels were observed.

Construction of the POMOF@Polypyrrole Composite with Enhanced Ion Diffusion and Capacitive Contribution for High-Performance Lithium-Ion Batteries.

Polyoxometalate (POM) as an "electronic sponge" can store a great number of electrons; however, shortcomings of poor conductivity and solubility in electrolytes cause a significant decrease in specific capacity and poor rate capability. To address the aforementioned disadvantages, a dual strategy was proposed, including coating the conductive polypyrrole (PPy) and utilizing nitrogenous ligands (1,10-phenanthroline monohydrate = 1,10-phen) for metal-organic frameworks (MOFs) to fabricate a [Cu(1,10-phen)(HO)][MoO]@PPy (Cu-POMOF@PPy) composite, effectively confining the POM in MOFs to avoid dissolution of POM in the electrolyte and improve electrochemical stability. Simultaneously, the PPy shell could improve the conductivity, contribute extra capacity, and alleviate volume variation of Cu-POMOF during cycling.

Ultrahigh Frequency and Anti-Interference Optical-Mode Resonance with Biquadratic Coupled FeCoB/Ru/FeCoB Trilayers.

Microwave soft magnetic films (SMFs) are the key materials to effectively miniaturize and multifunctionalize the microwave electromagnetic components and devices. However, currently, single-layer SMFs encounter a frequency bottleneck at around 10 GHz. The ferromagnet/nonmagnetic spacer/ferromagnet sandwiched films with strong interlayer exchange coupling are possible solutions to break through that frequency limitation because they exhibit ultrahigh optical-mode (OM) resonance frequency up to 50 GHz, while the tiny permeability and the limited thickness are their own obstacles to overcome.

Three-Dimensional Hierarchical Flowerlike FeP Wrapped with N-Doped Carbon Possessing Improved Li Diffusion Kinetics and Cyclability for Lithium-Ion Batteries.

Transition-metal phosphides have a potential application in lithium-ion batteries (LIBs) because of their high theoretical capacities and low cost; nevertheless, they possess dramatic volumetric variation during cycling associated with poor conductivity, limiting their practical applications. Here, a three-dimensional (3D) hierarchical flowerlike FeP coated with nitrogen-doped carbon layer (FeP@N,C hybrid) was constructed through a solvothermal method, followed by a phosphating approach under low temperature. N-doped carbon not only suppresses the volume fluctuation of FeP, but also promotes electron transfer, accompanied by catalyzing the decomposition of LiP to improve the reversibility of the FeP@N,C hybrid during cycling processes.

Improved Electrochemical Performance Based on Nanostructured SnS@CoS-rGO Composite Anode for Sodium-Ion Batteries.

A promising anode material composed of SnS@CoS flower-like spheres assembled from SnS nanosheets and CoS nanoparticles accompanied by reduced graphene oxide (rGO) was fabricated by a facile hydrothermal pathway. The presence of rGO and the combined merits of SnS and CoS endow the SnS@CoS-rGO composite with high conductivity pathways and channels for electrons and with excellent properties as an anode material for sodium-ion batteries (SIBs). A high capacity of 514.

Constructing Three-Dimensional Porous Carbon Framework Embedded with FeSe Nanoparticles as an Anode Material for Rechargeable Batteries.

Metal selenides have caused widespread concern due to their high theoretical capacities and appropriate working potential; however, they suffer from large volume variation during cycling and low electrical conductivity, which limit their practical applications. In this article, a three-dimensional (3D) porous carbon framework embedded with homogeneous FeSe nanoparticles (3D porous FeSe/C composite) was synthesized by a facile calcined approach, following a selenized method without a template. As the uniformity of FeSe nanoparticles and 3D porous structure are beneficial to accommodate volume stress upon cycling and shorten electrons/ions transport path, associated with carbon as a buffer matrix for increasing conductivity, the 3D porous FeSe/C composite displays excellent electrochemical properties with high reversible capacities of 798.

The effect of character contextual diversity on eye movements in Chinese sentence reading.

Chen, Huang, et al. (Psychonomic Bulletin & Review, 2017) found that when reading two-character Chinese words embedded in sentence contexts, contextual diversity (CD), a measure of the proportion of texts in which a word appears, affected fixation times to words. When CD is controlled, however, frequency did not affect reading times.

[Prokaryotic expression and characterization of two recombinant receptor-binding domain(RBD) proteins of human coronavirus NL63(HcoV-NL63)].

The receptor-binding domain(RBD) protein of HCoV-NL63 is a major target in the development of diagnostic assay and vaccine, it has a pivotal role in receptor attachment, viral entry and membrane fusion. In this study, we prepared 2 purified recombinant HCoV-NL63 RBD proteins using in E. coli system and identified the proteins by Western blotting.

Folding of an all-helical Greek-key protein monitored by quenched-flow hydrogen-deuterium exchange and NMR spectroscopy.

To advance our understanding of the protein folding process, we use stopped-flow far-ultraviolet (far-UV) circular dichroism and quenched-flow hydrogen-deuterium exchange coupled with nuclear magnetic resonance (NMR) spectroscopy to monitor the formation of hydrogen-bonded secondary structure in the C-terminal domain of the Fas-associated death domain (Fadd-DD). The death domain superfamily fold consists of six α-helices arranged in a Greek-key topology, which is shared by the all-β-sheet immunoglobulin and mixed α/β-plait superfamilies. Fadd-DD is selected as our model death domain protein system because the structure of this protein has been solved by NMR spectroscopy, and both thermodynamic and kinetic analysis indicate it to be a stable, monomeric protein with a rapidly formed hydrophobic core.

[Preparation and application of a novel HCV diagnostic antigen fused to streptavidin].

Objective: To prepare streptavidin-tagged hepatitis C virus (HCV) fusion protein and explore its application for the detection of antibody against HCV infection.

Methods: A recombinant plasmid pET-11d-C44P-SA was constructed, which coding a novel HCV diagnostic antigens (C44P) and streptavidin (SA) fusion protein, and the fusion protein was generated with BL21 (DE3) E Coli and identified by Western Blot analysis. Then the fusion protein was purified through the Ni-NTA affinity chromatography and over 90% purity has been achieved.

[Characterization and development of recombinant vaccinia viruses expressing different segments of spike protein derived from human coronavirus NL-63].

The spike (S) glycoprotein of HCoV-NL63 is a major target in the development of diagnostic assays and vaccines, but its antigenic and immunogenic properties remain unclear. Four fragments coding spike proteins (S1, S2, RL and RS) from HCoV-NL63 were amplified and cloned into the expression vector derived from vaccinia virus (Tiantan strain), and recombinant vaccinia viruses expressing four segments of spike proteins were generated (vJSC1175-S1; vJSC1175-S2; vJSC1175-RL; vJSC1175-RS), respectively. Their expression location in cell and level were characterized using indirect immune fluorescence assay (IFA) and Western-Blot, respectively.

[Expression and purification of different segments from HCoV-NL63 nucleocapsid protein and their application in detection of antibodies].

Prokaryotic expression plasmids carrying N-terminal(1-163aa) and C-terminal(141-306aa) gene of HCoV-NL63 nucleocapsid protein were constructed with pET-30a(+) vector. Consequently, we prepared two purified proteins, Np and Cp, respectively, and established a Western blotting-based line assay (WBLA) for detection of antibodies against HCoV-NL63 using three purified proteins: Np , Cp and Nf, a full-length HCoV-NL63 nucleocapsid protein as previously reported. We detected anti-HCoV-NL63 antibodies among 50 sera samples collected from adult for health-examination by WBLA.

Carbocyclic Sinefungin.

(3aS,4S,6R,6aR)-Tetrahydro-2,2-dimethyl-6-vinyl-3aH-cyclopenta[d][1,3]-dioxol-4-ol, itself available from ribose, provided a convenient entry point for an 18-step preparation of carbocyclic sinefungin. This procedure is adaptable to a number of carbocyclic sinefungin analogs with diversity of heterocyclic base and in the amino acid bearing side chain.