Publications by authors named "Guowei Zhong"

The survival rate of flap is a crucial factor for determining the success of tissue repair and reconstruction. Flap transplantation surgery often leads to ischemic and reperfusion injury, causing apoptosis and tissue necrosis, which significantly reduces the survival rate of flap. To address this issue, we developed a porcine skin decellularized matrix gel nanocomplex loaded with alprostadil (Alp) in Prussian blue nanoparticles (PB NPs) called Alp@PB-Gel.

View Article and Find Full Text PDF
Article Synopsis
  • Developed an automatic colony counting system using an enhanced image preprocessing method and a convolutional neural network (CNN), which effectively counts microorganisms in laboratory cultures from LED-illuminated photographs.
  • Introduced a light intensity correction algorithm to improve image clarity, enabling better differentiation of colonies from the media, leading to high accuracy in identifying the edges of Petri dishes and colony areas using U-Net models.
  • Achieved outstanding counting accuracy with 97.82% recovery through a systematic approach combining image preprocessing, U-Net segmentation for both dish edges and colonies, and a ResNet50 network for automatic counting, offering a significant advancement in automation and precision for microbiological studies.
View Article and Find Full Text PDF

This study aims to identify lactic acid bacteria (LAB) isolates possessing physiological characteristics suitable for use as probiotics in yogurt fermentation. Following acid and bile salt tolerance tests, Lactiplantibacillus plantarum (NUC08 and NUC101), Lacticaseibacillus rhamnosus (NUC55 and NUC201), and Lacticaseibacillus paracasei (NUC159, NUC216, and NUC351) were shortlisted based on intraspecies distribution for further evaluation. Their physiological probiotic properties, including transit tolerance, adhesion, autoaggregation, surface hydrophobicity, biofilm formation, and antibacterial activity, were assessed.

View Article and Find Full Text PDF