Three new cyclopentanoid monoterpenes from the roots of .

Three new cyclopentanoid monoterpenes, neopiscrocins A-C (-), together with 14 known compounds (-), were isolated from the roots of . The structres of these compounds were elucidated on the basis of their spectroscopic data. All compounds were evaluated for cytotoxicity against six human tumor cell lines (PC9, PANC1, HCT-116, Hep-G2, BGC-823, and MCF-7), hepatoprotective activity and anti-inflammatory activity.

Ten cyclopentanoid monoterpenes from the whole plant of Rehmannia piasezkii maxim.

Ten new cyclopentanoid monoterpenes (1-10) were isolated from the whole plant of Rehmannia piasezkii. The structures of these compounds were elucidated based on spectroscopic data analysis. In in-vitro assays, compounds 3, 7, and 9 exhibited weak hepatoprotective activities against APAP-induced HepG2 cell damage.

Two new iridoid glycosides from the whole plant of .

Two new iridoid glycosides, piasezkiiosides A () and B (), were isolated from aqueous extract of the whole plant of . Their structures were established from the spectroscopic data, chemical transformation, and X-ray diffraction analysis. Compound exhibited weak hepatoprotective activity against APAP-induced HepG2 cell damage.

Four new iridoid glycosides from the roots of .

Four new iridoid glycosides (-), rehmaglutosides L-O, were isolated from the air-dried roots of . Their structures were established from the spectroscopic data obtained and by chemical evidence. The known mellittoside () and ajugol () were also obtained in the current investigation, and the structure of mellittoside was unequivocally defined using X-ray diffraction data.

Seven new pentasaccharides from the roots of .

Seven new pentasaccharides (-), rehmaglupentasaccharides A-G, were isolated from the air-dried roots of . Their structures were established from the spectroscopic data obtained and by chemical evidence. The known verbascose () and stachyose () were also obtained in the current investigation, and the structure of stachyose was unequivocally defined using X-ray diffraction data.

Three new pyridine alkaloids and one new iridoid analogue from the leaves of .

As part of an ongoing project on species, three new pyridine alkaloides (glutinosines A - C), and one new iridoid analogue (rehmaglutin E), were isolated from the leaves of . The structures of the new compounds were established by extensive spectroscopic analysis and electronic circular dichroism calculations.

Four ionones and ionone glycosides from the whole plant of .

Four new ionones and ionone glycosides (-) were isolated from the whole plant of Maxim. Their planar structures as well as absolute configuration were confirmed via spectroscopic analysis, ECD calculation, and X-ray crystallography. Compounds - were tested for their cytotoxicity against five human tumor cell lines and ability to inhibit LPS-activated NO production in the BV2 cell line.

Cyclopentanoid monoterpenes from the whole plant of Rehmannia piasezkii maxim.

Twelve undescribed cyclopentanoid monoterpenes, Jiopiasins A-K and 6-epi-rehmaglutin A, and 19 known compounds were isolated from the whole plant of Rehmannia piasezkii Maxim. The structures of these compounds, especially their absolute stereochemistry, were established by extensive spectroscopic analysis, various quantum chemical calculations, and/or X-ray diffraction analyses. Furthermore, in vitro assays, some compounds exhibited moderate hepatoprotective activities against N-acetyl-p-aminophenol (APAP)-induced HepG2 cell damage.

[Chemical constituents from seeds of Hydnocarpus anthelminthica].

The present study investigated the chemical constituents from the dry seeds of Hydnocarpus anthelminthica. The compounds were isolated and purified from the dry seeds of H. anthelminthica by various chromatographic techniques including column chromatography over silica gel and Sephadex LH-20 and reversed-phase HPLC.

Five new cyclopentenyl fatty acid derivatives from the seeds of .

Five new fatty acids with a terminal 3-oxo-cyclopentene ring, cyclopentenone acids A-E (), were isolated from the ethanol extract of the seeds of . The structures of these compounds were elucidated on the basis of their spectroscopic data and chemical evidence. Compounds - were evaluated for their anti-inflammatory activity based on the inhibition of NO production in microglial BV2 cells, and all of them showed weak anti-inflammatory activities.

Three new erythrina alkaloids from the roots of .

Three new erythrina alkaloids, eryalkals A (), B (), and C (), were isolated from the roots of L. Their structures, including their absolute configurations, were elucidated based on analyses of HR-ESI-MS, 1D/2D NMR and single-crystal X-ray diffraction techniques. The isolated erythrina alkaloids were screened for the antioxidant and cytotoxic activities.

Bioactive bisbenzylisoquinoline alkaloids from the roots of Stephania tetrandra.

Ten new bisbenzylisoquinoline alkaloids (1-10) and eight known analogues (11-18) were obtained from the roots of Stephania tetrandra. The structures of these compounds were determined by spectroscopic methods, single-crystal X-ray diffraction, electronic circular dichroism analyses, and chemical method. Compounds 1, 15, and 16 showed the better anti-inflammatory activities with IC values of 15.

Aporphine and phenanthrene alkaloids with antioxidant activity from the roots of Stephania tetrandra.

Five new alkaloids (1-5), including three new aporphine alkaloids and two new phenanthrene alkaloids, together with 10 known compounds (6-15) were obtained from the roots of Stephania tetrandra. Their structures were elucidated by spectroscopic methods, single-crystal X-ray diffraction, and electronic circular dichroism analyses. Compounds 7-10, and 13 showed antioxidant activities with malondialdehyde (MDA) inhibitory rates of 62.

[Chemical compositions from roots of Erythrina corallodendron].

This project is to investigate chemical compositions from the roots of Erythrina corallodendron. Through the methods of silica gel,ODS,Sephadex LH-20 column chromatography and preparative HPLC,15 compounds were isolated from the 95% ethanol extract of the roots of E. corallodendron.

Five new iridoids from the whole plants of .

Five new iridoids (), jiohenrins A-E, together with sixteen known compounds (), were isolated from the whole plants of . The structures of these compounds were elucidated on the basis of their spectroscopic data.

Rearranged iridal-type triterpenoids from Iris tectorum.

Three new iridal-type triterpenoids (1-3) featuring a rearranged homofarnesylside chain were isolated from the rhizomes of Iris tectorum. Compounds 2 and 3 were found to be a pair of epimers. Their structures were elucidated on the basis of comprehensive spectroscopic analysis.

[Lignans from seed of Hydnocarpus anthelminthica].

This project is to investigate lignans from the seed of Hydnocarpus anthelminthica. Thirteen lignans were isolated from the 95% ethanol extract of the seed of H. anthelminthica, by polyamide resin, Sephadex LH-20, ODS column chromatography and preparative HPLC.

Nine new compounds from the whole plants of Rehmannia henryi.

Eight new iridoids (1-8) and an ionone glucoside (9), together with 31 known compounds (10-40), were isolated from the whole plants of Rehmannia henryi. The structures of these compounds were elucidated on the basis of their spectroscopic data and chemical evidence.

Heliosterpenoids A and B, two Novel Jatrophane-Derived Diterpenoids with a 5/6/4/6 Ring System from Euphorbia helioscopia.

Heliosterpenoids A and B (1 and 2), two unprecedented jatrophane-derived diterpenoid esters with a novel 5/6/4/6-fused tetracyclic ring skeleton, were isolated from the whole plants of Euphorbia helioscopia. The structures of 1 and 2 were elucidated by extensive spectroscopic methods and electronic circular dichroism (ECD) analyses. The plausible biogenetic pathways of 1 and 2 were postulated.

Aristolochic acid derivatives from the rhizome of Arisolochia championii.

Four new aristolochic acid derivatives aristchamic A (1), aristchamic B (2), aristochamic C (3a), aristchamic D (3b) and one new aristolactam aristolactam-CV (4), together with 10 known compounds (5-14), were isolated from the rhizomes of Aristolochia championii. Their structures were assigned by detailed analysis of MS and NMR spectroscopic data. All of the isolated compounds were evaluated for their cytotoxic activities against HCT-116, HepG2, BGC-823, NCI-H1650, and A2780 cell.

Apocynin derivatives from Iris tectorum.

Phytochemical investigation of the rhizomes of Iris tectorum resulted in the isolation and characterization of three new apocynin derivatives, apocynin-4-O-β-D-(6'-O-syringyl)glucopyranoside (1), scrophenoside C-7-ethyl ether (2, 3), together with a new naturally occurring compound but known by synthesis, apocynin-4-O-β-D-xylopyranoside (4), and five known ones (5-9). Their structures were elucidated on the basis of spectroscopic data interpretation.

Polycycloiridals with a Cyclopentane Ring from Iris tectorum.

Six new iridal-type triterpenoids containing an unprecedented cyclopentane ring, polycycloiridals E-J (1-6), were isolated from a large-scale re-extraction of Iris tectorum. A possible biosynthesis pathway is postulated. The known spirioiridotectal D (7) was also obtained in the current investigation, and its structure was unequivocally defined using X-ray diffraction data.

Four new compounds from the rhizome of Aristolochia championii.

Three new flavonoid glycosides (1-3) and one new aristololactam N-glycoside (4) were isolated from the rhizome of Aristolochia championii. The structures of compounds 1-4 were elucidated on the basis of their spectroscopic data and chemical evidence (UV, IR, HR-ESI-MS, 1D and 2D NMR). Their structures were determined as 8-(5-formyl-2-furanmethyl)-isorhamentin 3-O-robinobioside (1), 8-(5-methyl-2-furanoyl)-isorhamentin 3-O-robinobioside (2), 8-formylisorhamentin 3-O-robinobioside (3), and N-β-D-glucopyranosylaristololactam V (4), respectively.

Aromatic glycosides from the whole plants of Iris japonica.

Phytochemical investigation on the whole plants of Iris japonica led to the isolation of four new aromatic glycosides. Their structures including the absolute configurations were determined by spectroscopic and chemical methods as (-)-4-hydroxy-3-methoxy acetophenone 4-O-β-d-{6-O-[4-O-(7R,8S)-(4-hydroxy-3-methoxyphenylglycerol-8-yl)-3-methoxybenzoyl]}-glucopyranoside (1), (-)-4-hydroxy-3-methoxy acetophenone 4-O-β-d-{6-O-[4-O-(7S,8R)-(4-hydroxy-3-methoxyphenylglycerol-8-yl)-3-methoxybenzoyl]}-glucopyranoside (2), (-)-4-hydroxy-3-methoxy acetophenone 4-O-β-d-{6-O-[4-O-(7R,8R)-(4-hydroxy-3-methoxyphenylglycerol-8-yl)-3-methoxybenzoyl]}-glucopyranoside (3), (-)-4-hydroxy-3-methoxy acetophenone 4-O-β-d-{6-O-[4-O-(7S,8S)-(4-hydroxy-3-methoxyphenylglycerol-8-yl)-3-methoxybenzoyl]}-glucopyranoside (4), respectively.

Nine new compounds from the whole plants of Rehmannia chingii.

Nine new compounds, together with 16 known analogs, were isolated from the whole plants of Rehmannia chingii. The structures of compounds 1-9 were elucidated on the basis of their spectroscopic data and chemical evidence. In addition, the new compounds were tested for their hepatoprotective activities against APAP-induced HepG2 cell damage and their ability to inhibit LPS-induced nitric oxide production in the murine microglia BV2 cell line.