The Interaction between SARS-CoV-2 Nucleocapsid Protein and UBC9 Inhibits MAVS Ubiquitination by Enhancing Its SUMOylation.

Severe COVID-19 patients exhibit impaired IFN-I response due to decreased IFN-β production, allowing persistent viral load and exacerbated inflammation. While the SARS-CoV-2 nucleocapsid (N) protein has been implicated in inhibiting innate immunity by interfering with IFN-β signaling, the specific underlying mechanism still needs further investigation for a comprehensive understanding. This study reveals that the SARS-CoV-2 N protein enhances interaction between the human SUMO-conjugating enzyme UBC9 and MAVS.

An individualized criterion for left bundle branch capture in patients with a narrow QRS complex.

Background: Left bundle branch (LBB) pacing (LBBP) is a physiological pacing; however, the accuracy of current electrocardiographic criteria for LBBP remains inadequate.

Objective: The purpose of this study was to establish a novel individualized criterion to improve the accuracy of LBBP determination in patients with a narrow QRS complex.

Methods: Patients in whom both LBBP and left ventricular septal pacing (LVSP) were acquired during operation were enrolled.

Effect of greenhouse gas emissions on the life cycle of biomass energy production and conversion under different straw recycling modes.

With the gradual growth of greenhouse gas (GHG) emissions during the agricultural cultivation cycle, GHG emissions specific to the production and conversion of biomass energy is becoming increasingly problematic. Current studies lack analysis of net GHG emissions generated during full life cycle of agricultural cultivation, straw use and bioenergy production. This study measures the global warming potential of biomass energy production and conversion processes under different agricultural cultivation cycle systems based on life cycle approach, accompanied by four straw treatment methods: fast pyrolysis, slow pyrolysis, flash pyrolysis and anaerobic fermentation.

Influencing Effects of Fabrication Errors on Performances of the Dielectric Metalens.

Despite continuous developments of manufacturing technology for micro-devices and nano-devices, fabrication errors still exist during the manufacturing process. To reduce manufacturing costs and save time, it is necessary to analyze the effects of fabrication errors on the performances of micro-/nano-devices, such as the dielectric metasurface-based metalens. Here, we mainly analyzed the influences of fabrication errors in dielectric metasurface-based metalens, including geometric size and shape of the unit element, on the focusing efficiency and the full width at half maximum (FWHM) values.

Polymerizable Surfactant Ligand for Stabilization and Film Formation of CsPbBr Nanocrystals.

Surfactant ligands are important in the synthesis of inorganic perovskite nanocrystals (NCs), not only for stabilizing NCs but also for surface defect passivation. A new polymerizable surfactant ligand with a multidentate l-cysteine head, a long oleoyl tail, and a polymerizable styrenyl group (NOSVC) is designed for the post-synthesis treatment and stabilization of colloidal CsPbBr NCs in this work. H nuclear magnetic resonance and X-ray photoelectron spectroscopy analysis show that the l-cysteine head has strong interactions with the NCs.

Bilateral septal pacing in combination with coronary venous pacing for cardiac resynchronization therapy.

Background: Conventional right ventricular pacing combined with coronary venous pacing (CVP) is a mainstay for cardiac resynchronization therapy (CRT). However, QRS duration of conventional CRT may be frequently more than 130 ms. This study aimed to evaluate the effectiveness of QRS narrowing by bilateral septal pacing (BSP) in combination with CVP for CRT (BSP-CRT).

Morphologies and dynamics of free surfaces of crystals composed of active particles.

Active matter exhibits various collective motions and nonequilibrium phases, such as crystals; however, their surface properties have been poorly explored. Here, we use Brownian dynamics simulations to investigate the surface morphology and dynamics of two-dimensional active crystals during and after growth. For crystal growth on a substrate, the position and roughness of the crystal surface reach steady states at different times.

Propagation dynamics of electrotactic motility in large epithelial cell sheets.

Directional migration initiated at the wound edge leads epithelia to migrate in wound healing. How such coherent migration is achieved is not well understood. Here, we used electric fields to induce robust migration of sheets of human keratinocytes and developed an model to characterize initiation and propagation of epithelial collective migration.

Comparing the cost-benefit probability of management based on early-stage and late-stage economic thresholds with that of seed treatment of Aphis glycines.

Background: The current integrated pest management (IPM) curative strategy for soybean aphid, Aphis glycines Matsumura (Hemiptera: Aphididae), relies on responsive spraying foliar insecticides during the R1-R5 soybean stage when aphid abundance reaches the economic threshold (ET) of 250 aphids plant (traditional IPM). By analyzing the relationship between aphid abundance and yield loss before the R1 stage, we developed an early-stage ET. We propose to spray foliar insecticides on plants colonized with aphids using the early-stage ET as a trigger (improved IPM), together with seed treatment to manage A.

Morphologies and dynamics of the interfaces between active and passive phases.

Active matters exhibit interesting collective behaviors and novel phases, which provide an important platform for the study of nonequilibrium physics. Mixtures of active and passive particles have been intensively studied in motility-induced phase separation, but the morphology of the active-passive interface has been poorly explored. In this work, we investigate the interface morphology in two-dimensional mixtures of active and passive particles using Brownian dynamics simulations.

Neural basis of implicit cognitive reappraisal in panic disorder: an event-related fMRI study.

Background: Panic disorder (PD) is thought to be related with deficits in emotion regulation, especially in cognitive reappraisal. According to the cognitive model, PD patients' intrinsic and unconscious misappraisal strategies are the cause of panic attacks. However, no studies have yet been performed to explore the underlying neuromechanism of cognitive reappraisal that occur on an unconscious level in PD patients.

Performance of single-stage partial nitritation and anammox reactor treating low-phenol/ammonia ratio wastewater and analysis of microbial community structure.

Phenol and ammonia are common pollutants in many industrial wastewaters. The partial nitritation and anammox process is a very promising technology for treating phenol-ammonia wastewater. This study was the first time to rapidly achieve the start-up and operation of the single-stage partial nitritation /anammox reactor treating phenol-ammonia wastewater.

Intermittent Hypoxia Exposure Can Prevent Reductions in Hemoglobin Concentration After Intense Exercise Training in Rats.

Intense exercise training can induce low concentrations of hemoglobin, which may be followed by maladaptation. Therefore, it is important for athletes to prevent low concentrations of hemoglobin during intense exercise training. In this study, we explored whether different protocols of intermittent hypoxic exposure (IHE, normobaric hypoxia, 14.

Circulating Soluble ST2 Predicts All-Cause Mortality in Severe Heart Failure Patients with an Implantable Cardioverter Defibrillator.

Background: Heart failure (HF) is the terminal stage of all cardiovascular events. Although implantable cardioverter defibrillator (ICD) therapies have reduced mortality among the high-risk HF population, it is necessary to determine whether certain factors can predict mortality even after cardiac device implantation. Growth stimulation expressed gene 2 (ST2) is an emerging biomarker for HF patient stratification in different clinical settings.

A simple and practical criterion for determining a failed His-bundle pacing.

Aims: To establish a simple criterion for determining a failed His-bundle pacing (HBP). This criterion states that if stimulus to QRS end interval is longer than His-bundle potential to QRS end interval ('S-QRSend > H-QRSend') then a failed HBP can be determined.

Methods And Results: We performed retrospective analysis on 737 pacing tests around His-bundle in 241 patients and prospective analysis on 400 tests in 123 patients.

[General Troubleshooting and Preventive Maintenance of the Digestive Electron Microscope System].

Objective: Improve the integrity of the digestive electron microscope equipment and reduce the cost of equipment failure maintenance.

Methods: By studying the composition and function of the digestive electron microscope system and analyzing the causes of common faults, a targeted preventive maintenance plan is developed, equipment users are graded, and a training system is established.

Results: The user of the device can skillfully analyze the cause of the malfunction and timely deal with the sudden failure of the diagnosis and treatment, thereby reduce the risk of diagnosis and treatment and the investment in hospital maintenance.

Absorption, accumulation and distribution of metals and nutrient elements in poplars planted in land amended with composted sewage sludge: A field trial.

Interest in the application of sewage sludge as amendments to grow trees has continued to increase, especially for fast-growing trees such as poplars. In this study, two-year field trial was conducted to determine the effects of compost sewage sludge (CSS) soil application on the distributions of metal and nutrient elements in poplars (Populus × euramericana 'Guariento') and poplar growth. Soil was amended with one of four CSS treatments in both study years: control (2012, 2013: 0 t/ha), SS1 (2012: 7.

A High-Sensitivity Microfluidic Sensor Based on a Substrate Integrated Waveguide Re-Entrant Cavity for Complex Permittivity Measurement of Liquids.

In this study, a novel non-invasive and contactless microwave sensor using a square substrate integrated waveguide (SIW) re-entrant cavity is proposed for complex permittivity measurement of chemical solutions. The working principle of this sensor is based on cavity perturbation technique, in which the resonant properties of cavity are utilized as signatures to extract the dielectric information of liquid under test (LUT). A winding microfluidic channel is designed and embedded in the gap region of the cavity to obtain a strong interaction between the induced electric field and LUT, thus achieving a high sensitivity.

Preparation of Protein Molecular-Imprinted Polysiloxane Membrane Using Calcium Alginate Film as Matrix and Its Application for Cell Culture.

Bovine serum albumin (BSA) molecular-imprinted polysiloxane (MIP) membrane was prepared by sol-gel technology, using silanes as the functional monomers, BSA as the template and CaAlg hydrogel film as the matrix. The stress-strain curves of wet CaAlg membrane and molecular-imprinted polysiloxane membrane were investigated. We evaluate the adsorption and recognition properties of MIP membrane.

The interaction between BSA and DOTAP at the air-buffer interface.

In this article, the interaction between bovine serum albumin (BSA) and the cationic 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) at the air-buffer interface was investigated at different subphase's pH values (pH = 3, 5 and 10). Surface pressure measurements (π - A) and penetration kinetics process (π - t) were carried out to reveal the interaction mechanism and the dynamical behavior. The data showed that π - A isotherms moved towards larger mean molecular area when the concentration of BSA ([BSA]) increased, the amount of BSA adsorbed onto DOTAP monolayer reached a threshold value at a [BSA] of 5 × 10 M, and BSA desorbed from the lipid monolayer as time goes by.

Investigation of Surface Behavior of DPPC and Curcumin in Langmuir Monolayers at the Air-Water Interface.

Langmuir monolayers of 1,2-dipalmitoyl--glycero-3-phosphocholine (DPPC) and a mixture of DPPC with curcumin (CUR) have been investigated at the air-water interface through a combination of surface pressure measurements and atomic force microscopy (AFM) observation. By analyzing the correlation data of mean molecular areas, the compressibility coefficient, and other thermodynamic parameters, we obtained that the interaction between the two components perhaps was mainly governed by the hydrogen bonding between the amino group of DPPC and the hydroxyl groups of CUR. CUR markedly affected the surface compressibility, the thermodynamic stability, and the thermodynamic phase behaviors of mixed monolayers.

Effects of Concentration and Surface Pressure on MBP Interaction with Cholesterol in Langmuir Films.

Predicting the mechanism of MBP binding to cholesterol is meaningful in understanding how MBP participate in lateral membrane organization. The interaction of MBP with cholesterol monolayer was investigated at three surface pressures on 10 mM Tris-HCl buffer with the different concentrations of MBP. The results show that - isotherms shift to larger molecular area at all pressures.