Publications by authors named "Guoping Da"
Histone demethylase LSD1 is a flavin-dependent amine oxidase that catalyzes the oxidative removal of one or two methyl groups from the methyl-lysine-4 side chain of histone H3. We have designed and synthesized two peptide-based inhibitor analogues that block LSD1. One of these inhibitors, compound 1, contains a propargylamine functionality and shows time-dependent inactivation of LSD1.
View Article and Find Full Text PDFThe SWIRM domain is a module found in the Swi3 and Rsc8 subunits of SWI/SNF-family chromatin remodeling complexes, and the Ada2 and BHC110/LSD1 subunits of chromatin modification complexes. Here we report the high-resolution crystal structure of the SWIRM domain from Swi3 and characterize the in vitro and in vivo function of the SWIRM domains from Saccharomyces cerevisiae Swi3 and Rsc8. The Swi3 SWIRM forms a four-helix bundle containing a pseudo 2-fold axis and a helix-turn-helix motif commonly found in DNA-binding proteins.
View Article and Find Full Text PDFArticle Synopsis
- The Bre5 protein acts as a cofactor for the deubiquitinating enzyme Ubp3, and its NTF2-like domain is essential for Ubp3's functionality due to its unique structure and presence of an intermolecular disulfide bond.
- Under non-reducing conditions, the Bre5 NTF2-like domain forms stable dimers, while a mutant version that lacks the disulfide bond shows a reversible shift between monomer and dimer forms, indicating their potential role in modulating Bre5's function.
- Novel findings reveal that Bre5 and Ubp3 form a 2:1 complex and identify a specific region on Bre5 important for binding Ubp3, which enhances our understanding of how Bre5