Publications by authors named "Guoning DU"
[Analysis of the salt-stress responsive element of the promoter of peanut small GTP binding protein gene ].
Sheng Wu Gong Cheng Xue Bao
August 2022
To study the molecular mechanism of salt stress response of peanut small GTP binding protein gene , a 1 914 bp promoter fragment upstream of the start codon of gene (-P) from peanut was cloned. Subsequently, five truncated fragments (-P1--P5) with lengths of 1 729, 1 379, 666, 510 and 179 bp were obtained through deletion at the 5' end, respectively. Plant expression vectors where these six promoter fragments were fused with the gene were constructed and transformed into tobacco by -mediated method, respectively.
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- Auxin response factors (ARFs) are key transcription factors involved in regulating genes that respond to auxin, a plant growth hormone, and this study identified 29 ARFs in A. duranensis and 30 in A. ipaensis.
- The research included characterizing the classifications, conserved domains, and evolution of these ARFs, revealing some to be responsive to abiotic stresses like high salinity and biotic stress from Bradyrhizobium infection.
- The study also found that overexpressing the microRNA miR160 could degrade a specific ARF gene, enhancing salt tolerance in transgenic peanuts, which highlights the potential for further research into ARFs' roles in plant stress responses.
Article Synopsis
- * This study identified 64 nsLTP genes in A. duranensis, categorized into six subfamilies, and revealed that evolutionary duplications have influenced their distribution across the plant's chromosomes.
- * The research also found that some AdLTPs are linked to resistance against nematode infections and that environmental factors like salinity and temperature affect their expression, offering insights for developing stress-resistant peanut varieties.