Integration of paper-based colorimetric microdevice and magnetic nanoparticles affinity for high-throughput capture of antimicrobial resistance-reversing agent from complex natural products.

Efficient analysis of active ingredient in complex natural products is crucial for drug discovery, but developing a simple method for this is challenging. The discovery of drugs against bacterial resistance is urgent because drug-resistant bacteria produce β-lactamases, which inactivate antibiotics and increase infection risks, particularly the AmpC β-lactamase. Here, an integrated analytical model based on colorimetric sensing and magnetic nanoparticles (MNPs) affinity chromatography was developed for screening AmpC β-lactamase inhibitors.

Immunoproteomic analysis and identification of possible allergenic proteins in Artemisia annua pollen.

Background: Artemisia annua (A. annua) is a wind-pollinated weed and a major allergen responsible for allergic respiratory diseases in Northern China.

Methods: This study involved the separation of pollen proteins from A.

Development of a pre-column derivatization ultra-high-performance liquid chromatography method for polysaccharide and monosaccharide quantification in Lycium barbarum.

Given the limited specificity and accuracy observed in the current official colorimetric quantification of polysaccharide in Lycium barbarum, our study aims to establish a novel, specific, accurate, and economic pre-column derivatization ultra-high-performance liquid chromatography (UHPLC) method for determining the monosaccharide and polysaccharide content in L. barbarum. The optimization of extraction, hydrolysis, and derivatization (using 1-phenyl-3-methyl-5-pyrazolone) processes for polysaccharide from L.

Self-polymerization silica nanoparticles based molecularly imprinted polymers for selective recognition of protein.

Molecularly imprinted polymers (MIPs) are promising for precise protein separation and purification. However, challenges persist due to their large size, variable configuration, and instability during preparation. Here, a simple silicon self-assembly program was designed to synthesize MIPs without any organic reagents and acid-base catalysis, avoiding the structural damage of protein under severe conditions.

A strategy for inhibitors screening of xanthine oxidase based on colorimetric sensor combined with affinity chromatography technology.

The discovery of enzyme inhibitors from natural products is a crucial aspect in the development of therapeutic drugs. However, the complexity of natural products presents a challenge in developing simple and efficient methods for inhibitor screening. Herein, we have developed an integrated analytical model for screening xanthine oxidase (XOD) inhibitors that combines simplicity, accuracy, and efficiency.

GPU-Accelerated RSF Level Set Evolution for Large-Scale Microvascular Segmentation.

Microvascular networks are challenging to model because these structures are currently near the diffraction limit for most advanced three-dimensional imaging modalities, including confocal and light sheet microscopy. This makes semantic segmentation difficult, because individual components of these networks fluctuate within the confines of individual pixels. Level set methods are ideally suited to solve this problem by providing surface and topological constraints on the resulting model, however these active contour techniques are extremely time intensive and impractical for terabyte-scale images.

DNA-functionalized MOF fluorescent probes for the enzyme-free and pretreatment-free detection of MicroRNA in serum.

MicroRNA (miRNA) is a promising biomarker that plays an important role in various biomedical applications, especially in cancer diagnosis. However, the current miRNA detection technology has inherent limitations such as complex operation, expensive testing cost and excessive detection time. In this study, a dual signal amplification biosensor based on DNA-functionalized metal-organic frameworks (MOFs) fluorescent probes, MFPBiosensor, was established for the enzyme-free and pretreatment-free detection of the colon cancer (CC) marker miR-23a.

Establishment and application of a screening method for α-glucosidase inhibitors based on dual sensing and affinity chromatography.

α-Glucosidase plays a direct role in the metabolic pathways of starch and glycogen, any dysfunction in its activity could result in metabolic disease. Concurrently, this enzyme serves as a target for diverse drugs and inhibitors, contributing to the regulation of glucose metabolism in the human body. Here, an integrated analytical method was established to screen inhibitors of α-glucosidase.

Hybrid Base Complex: Extract and Visualize Structure of Hex-dominant Meshes.

Hex-dominant mesh generation has received significant attention in recent research due to its superior robustness compared to pure hex-mesh generation techniques. In this work, we introduce the first structure for analyzing hex-dominant meshes. This structure builds on the base complex of pure hex-meshes but incorporates the non-hex elements for a more comprehensive and complete representation.

Versatile Mesoporous All-Wood Sponge Enabled by In Situ Fibrillation toward Indoor-Outdoor Energy Management and Conversion.

The on-demand regulation of cell wall microstructures is crucial for developing wood as a functional building material for energy management and conversion. Here, a novel strategy based on reactive deep eutectic solvent is developed to one-step in situ fibrillate wood via disrupting the hydrogen bonding networks in cell walls and simultaneously carboxylating wood components, without significantly altering the native hierarchical structures of wood. Benefiting from its distinctive cell wall structure composed of individualized yet well-organized lignocellulose nanofibrils, in situ fibrillated wood exhibits a prominent mesoporous structure with a specific surface area of 81 m/g.

Extract and Characterize Hairpin Vortices in Turbulent Flows.

Hairpin vortices are one of the most important vortical structures in turbulent flows. Extracting and characterizing hairpin vortices provides useful insight into many behaviors in turbulent flows. However, hairpin vortices have complex configurations and might be entangled with other vortices, making their extraction difficult.

Exposed {110} facets of BiOBr anchored to marigold-like MnCoO with abundant interfacial electron transfer bridges and efficient activation of peroxymonosulfate.

Precise charge transfer modification and efficient activation of peroxymonosulfate are effective methods for increasing photocatalytic efficiency. Here, BiOBr/MnCoO photocatalysts with abundant Mn-Br bonds were generated by immobilizing the exposed {110} facets of BiOBr in the marigold-like MnCoO. The prepared BiOBr/MnCoO retained the marigold-like morphology of MnCoO while exhibiting good adsorption properties and interface contact effects.

Study on Degradation of 1,2,4-TrCB by Sugarcane Cellulose-TiO Carrier in an Intimate Coupling of Photocatalysis and Biodegradation System.

1,2,4 trichlorobenzene (1,2,4-TrCB) is a persistent organic pollutant with chemical stability, biological toxicity, and durability, which has a significant adverse impact on the ecological environment and human health. In order to solve the pollution problem, bagasse cellulose is used as the basic framework and nano TiO is used as the photocatalyst to prepare composite carriers with excellent performance. Based on this, an intimate coupling of photocatalysis and biodegradation (ICPB) system combining photocatalysis and microorganisms is constructed.

Catalytic removal of attached tetrabromobisphenol A from microplastic surface by biochar activating oxidation and its impact on potential of disinfection by-products formation.

There are numerous studies concerning the impacts of widespread microplastic pollution on the ecological environment, and it shows synergistic effect of microplastics and co-exposed pollutants in risk enhancement. However, the control methods for removing harmful pollutants from microplastic surface to reduce their ecological toxicity has rarely been explored. In this paper, magnetic graphitized biochar as a catalyst is shown to achieve 97% removal of tetrabromobisphenol A (TBBPA) from microplastics by biochar mediated electron transfer.

Sulfated lignocellulose nanofibril based composite aerogel towards adsorption-photocatalytic removal of tetracycline.

The design-on-demand of lignocellulose nanofibril-based materials for contaminant disposal is worth exploring. Herein, we mildly extract sulfated lignocellulose nanofibrils from bagasse via a deep eutectic solvent-based approach, and use them as a matrix for TiO nanoparticles (TNPs) towards adsorption-photocatalytic synergistic removal of tetracycline (TC). The resultant lignocellulose-based nanocomposite aerogel possessing a high specific surface area (95.

Hemicellulose-rich transparent wood: Microstructure and macroscopic properties.

Hemicellulose plays a vital role in nature wood matrix. Here, we first demonstrate the significance of hemicellulose for improving the microstructure and macroscopic properties of transparent wood (i.e.

A Cellulose-Type Carrier for Intimate Coupling Photocatalysis and Biodegradation.

Intimate coupling photocatalysis and biodegradation treatment technology is an emerging technology in the treatment of refractory organic matter, and the carrier plays an important role in this technology. In this paper, sugarcane cellulose was used as the basic skeleton, absorbent cotton was used as a reinforcing agent, anhydrous sodium sulfate was used as a pore-forming agent to prepare a cellulose porous support with good photocatalytic performance, and nano-TiO was loaded onto it by a low-temperature bonding method. The results showed that the optimal preparation conditions of cellulose carriers were: cellulose mass fraction 1.

Study of the Preparation and Properties of Chrysin Binary Functional Monomer Molecularly Imprinted Polymers.

Chrysin is a natural bioactive molecule with various groups, and it has been a challenge to separate and enrich chrysin from natural products. Molecularly imprinted polymers have been widely used in the extraction of natural products, but the number and type of functional monomers limits the separation effect. The synergistic action of multiple functional monomers can improve the separation effect.

Enzyme Encapsulation by Facile Self-Assembly Silica-Modified Magnetic Nanoparticles for Glucose Monitoring in Urine.

Silica nanoparticles hold tremendous potential for the encapsulation of enzymes. However, aqueous alcohol solutions and catalysts are prerequisites for the production of silica nanoparticles, which are too harsh for maintaining the enzyme activity. Herein, a procedure without any organic solvents and catalysts (acidic or alkaline) is developed for the synthesis of silica-encapsulated glucose-oxidase-coated magnetic nanoparticles by a facile self-assembly route, avoiding damage of the enzyme structure in the reaction system.

Biofilm response and removal via the coupling of visible-light-driven photocatalysis and biodegradation in an environment of sulfamethoxazole and Cr(VI).

The widespread contamination of water systems with antibiotics and heavy metals has gained much attention. Intimately coupled visible -light-responsive photocatalysis and biodegradation (ICPB) provides a novel approach for removing such mixed pollutants. In ICPB, the photocatalysis products are biodegraded by a protected biofilm, leading to the mineralization of refractory organics.

Effects of different -acyl-serine lactone signaling molecules on the performance of anaerobic granular sludge.

Exogenous addition of acyl-homoserine lactone (AHL) signaling molecules can improve or inhibit the methane production performance of anaerobic granular sludge (AnGS) by quorum sensing (QS). To explore the specific effect of AHLs on AnGS, 2 μM of signal molecules were added to the reactor and we analyzed their effects on AnGS biodiversity, extracellular polymeric substance (EPS), specific methanogenic activity (SMA) and chemical oxygen demand (COD) removal rate of AnGS. The results indicated that the four types of AHLs improve the COD removal rate, SMA and organic composition of AnGS.

Cationic Lignocellulose Nanofibers from Agricultural Waste as High-Performing Adsorbents for the Removal of Dissolved and Colloidal Substances.

The accumulation of dissolved and colloidal substances (DCS) in the increasingly closed paper circulating water system can seriously lower the productivity and safety of papermaking machines, and it has been a challenge to develop an adsorbent with low cost, high adsorption efficiency and large adsorption capacity for DCS removal. In this study, cationic lignocellulose nanofibers (CLCNF) were obtained by cationic modification of agricultural waste bagasse in deep eutectic solvents (DES) followed by mechanical defibrillation, and then CLCNF were employed as an adsorbent for DCS model contaminant polygalacturonic acid (PGA) removal. CLCNF was characterized by transmission electron microscopy, Fourier transform infrared, elemental analysis, X-ray diffraction, and thermogravimetric analysis.