Contactless Photoelectrochemical Biosensor Based on the Ultraviolet-Assisted Gas Sensing Interface of Three-Dimensional SnS Nanosheets: From Mechanism Reveal to Practical Application.

This work reports a contactless photoelectrochemical biosensor based on an ultraviolet-assisted gas sensor (UV-AGS) with a homemade three-dimensional (3D)-SnS nanosheet-functionalized interdigitated electrode. After rigorous examination, it was found that the gas responsiveness accelerated and the sensitivity increased using the UV irradiation strategy. The effects of the interlayer structure and the Schottky heterojunction on the gas-sensitive response of O and NH under UV irradiation were further investigated theoretically by 3D electrostatic field simulations and first-principles density functional theory to reveal the mechanism.

Pressure-Based Biosensor Integrated with a Flexible Pressure Sensor and an Electrochromic Device for Visual Detection.

This work demonstrated a pressure-based biosensor integrated with a flexible pressure sensor and an electrochromic device for visual detection. Initially, a sandwich-type immunoreaction for target carcinoembryonic antigen (CEA, as a model analyte) was carried out using the capture antibody (cAb) and platinum nanoparticles-labeled detection antibody (PtNPs-dAb) in a reaction cell. The added hydrogen peroxide (HO) could be catalyzed by the PtNPs to generate oxygen (O).

Actuating photoelectrochemical sensing sensitivity coupling core-core-shell FeO@C@TiO with molecularly imprinted polypyrrole.

Magnetic photocatalyst coupling with molecular imprinting technique is an efficient method for the specific photodegrade organic pollutants. Herein, this method is applied to fabricate a photoelectrochemical sensing platform for bisphenol A (BPA) detection based on electro-polymerization of molecularly imprinting pyrrole (MI-PPy) on the core-shell magnetic nanoparticles, FeO@C@TiO, which is magnetically adsorbed on magnetic glassy carbon electrode (MGCE). The MI-PPy layer not only provides molecular recognition capabilities for selective absorption of BPA, but also improves the photoelectrochemical behavior because of the heterostructure of TiO/PPy that accelerated photoelectron transfer, which is a strategy to kill two birds with one stone.

Platinum Nanozyme-Triggered Pressure-Based Immunoassay Using a Three-Dimensional Polypyrrole Foam-Based Flexible Pressure Sensor.

This work describes a novel and portable pressure-based point-of-care (POC) testing strategy for the sensitive and rapid detection of carcinoembryonic antigen (CEA) via a flexible pressure sensor constructed by three-dimensional (3D) polypyrrole (PPy) foam. Initially, platinum nanoparticles (PtNPs) were conjugated to the detection antibodies, which were used to form sandwich-type immunocomplexes with targets and capture antibodies in the reaction cell. Then, the carried PtNPs catalyzed the dissociation of hydrogen peroxide (HO) for the generation of oxygen (O) in a sealed device, translating the biomolecule recognition event into gas pressure.

TiC MXene quantum dot-encapsulated liposomes for photothermal immunoassays using a portable near-infrared imaging camera on a smartphone.

Methods based on the photothermal effect (a common phenomenon in nature) have been widely applied in different fields; however, their application in bioanalysis has lagged behind. Herein, we designed a near-infrared (NIR) photothermal immunoassay for the qualitative or quantitative detection of prostate-specific antigen (PSA) using titanium carbide (TiC) MXene quantum dot (QD)-encapsulated liposomes with high photothermal efficiency. This system involves a sandwich-type immunoreaction and photothermal measurements.

Saw-Toothed Microstructure-Based Flexible Pressure Sensor as the Signal Readout for Point-of-Care Immunoassay.

A saw-toothed microstructure-based flexible pressure sensor was designed as the signal readout for point-of-care (POC) immunoassay of carcinoembryonic antigen (CEA). In this portable POC bioassay, the sandwich-type immunoreaction was first carried out to capture the target on a microplate, which simultaneously introduced the platinum nanoparticles (PtNPs). Upon adding hydrogen peroxide (HO), the pressure as the bridge between the molecular recognition event and detectable signal was increasing rapidly, resulting from the decomposition of HO accelerated by PtNPs.

Paper Electrode-Based Flexible Pressure Sensor for Point-of-Care Immunoassay with Digital Multimeter.

A novel paper electrode-based flexible pressure sensor modified with multiwalled carbon nanotubes was designed for point-of-care (POC) immunoassay of carcinoembryonic antigen (CEA) with digital multimeter readout. The portable POC testing device consisted of flexible pressure sensor equipped with a paper electrode and connected through syringe tubing to a single-break microplate. The immunoreaction was initially carried out on the microplate with a sandwich-type assay format using platinum nanozyme-labeled secondary antibody for the gas generation.

Metal-Polydopamine Framework: An Innovative Signal-Generation Tag for Colorimetric Immunoassay.

In this work, an innovative enzyme-free colorimetric immunoassay was proposed for the sensitive detection of alpha-fetoprotein (AFP) by introducing thymolphthalein-modified metal-polydopamine framework (MPDA@TP) for the signal generation and amplification. Using zeolitic imidazolate framework (ZIF-67) as the template, the hollow-structured metal-polydopamine framework (MPDA) with high surface recovery and abundant groups was synthesized and functionalized with thymolphthalein (TP) molecules via typical π-stacking reaction. In the presence of target AFP, an MPDA@TP-linked immunosorbent assay (MLISA) was implemented on the capture antibody-modified microplate by using detection antibody-labeled MPDA@TP as the secondary antibody.

A new enzyme immunoassay for alpha-fetoprotein in a separate setup coupling an aluminium/Prussian blue-based self-powered electrochromic display with a digital multimeter readout.

A new enzyme immunosensing platform was designed for the simple, rapid and sensitive detection of disease-related biomarkers (alpha-fetoprotein, AFP, was used as a model in this case), coupling an aluminium (Al)/Prussian blue-based self-powered electrochromic display with a digital multimeter readout.

Exciton-Plasmon Interaction between AuNPs/Graphene Nanohybrids and CdS Quantum Dots/TiO for Photoelectrochemical Aptasensing of Prostate-Specific Antigen.

A competitive-displacement reaction strategy based on target-induced dissociation of gold nanoparticle coated graphene nanosheet (AuNPs/GN) from CdS quantum dot functionalized mesoporous titanium dioxide (CdS QDs/TiO) was designed for the sensitive photoelectrochemical (PEC) aptasensing of prostate-specific antigen (PSA) through the exciton-plasmon interaction (EPI) between CdS QDs and AuNPs. To construct such an aptasensing system, capture DNA was initially conjugated covalently onto CdS QDs/TiO-modified electrode, and then AuNPs/GN-labeled PSA aptamer was bound onto biofunctionalized CdS QDs/TiO via hybridization chain reaction of partial bases with capture DNA. Introduction of AuNPs/GN efficiently quenched the photocurrent of CdS QDs/TiO thanks to energy transfer.

Semiautomated Support Photoelectrochemical Immunosensing Platform for Portable and High-Throughput Immunoassay Based on Au Nanocrystal Decorated Specific Crystal Facets BiVO Photoanode.

Photoelectrochemical (PEC) measurement has been developed rapidly for bioanalysis in recent years. However, the actual application for most existed PEC bioanalytical systems is still a challenge because the perfect solutions for sensing surface design, high-throughput detection, and portability are lacked. To successfully overcome these limitations and realize accurate, continuous screening and assessing on prognostic indicator of early stage cancer on the spot, an innovative and portable semiautomated support power-free photoelectrochemical (SP-PEC) immunosensing platform consisted with a miniature semiautomatic injection system and digital multimeter (DMM) readout is designed (prostate specific antigen, PSA, was used as the proof-of-concept analyte).

Systems pharmacology of mifepristone (RU486) reveals its 47 hub targets and network: comprehensive analysis and pharmacological focus on FAK-Src-Paxillin complex.

Mifepristone (RU486), a synthetic steroid compound used as an abortifacient drug, has received considerable attention to its anticancer activity recently. To explore the possibility of using mifepristone as a cancer metastasis chemopreventive, we performed a systems pharmacology analysis of mifepristone-related molecules in the present study. Data were collected by using Natural Language Processing (NLP) and 513 mifepristone-related genes were dug out and classified functionally using a gene ontology (GO) hierarchy, followed by KEGG pathway enrichment analysis.