Multifunctional Au-Ag NCs for luminescence and colorimetric double signal sensing of HS and catalytic reduction of nitrophenol.

In this study, the N-Acetyl-l-Cysteine (NAC)-capped gold and silver bimetallic nanoclusters (NAC@Au-Ag NCs) was synthesized by reflux method. Due to the silver effect, the NAC@Au-Ag NCs exhibited strong photoluminescence at far-red/near-infrared regions and better catalytic performance than Au or Ag NCs. Upon addition of HS, the Au-Ag NCs exhibited obvious fluorescence quench and color changes through the generation of metal sulfides and a static quenching process.

A specific dual-locked fluorescence probe to visualize the dynamic changes of lipid droplets and hypochlorous acid in inflammation.

Inflammation is a key factor leading to the occurrence and development of many diseases, both lipid droplets (LDs) and hypochlorous acid (HClO/ClO) are regarded as the important biomarkers of inflammation. Therefore, it is of great significance to develop an efficient single chemical sensor that can simultaneously detect these two biomarkers. To achieve the goal, we developed a dual-locked fluorescence probe (TPA-DNP) by fusing two targets activated reporting system, its implementation was achieved by turning-on the fluorescence of TPA-DNP through LDs and HClO/ClO simultaneously.

Full-length transcriptomics study of Ustiloxins-induced hepatocyte injury.

Ustiloxins is a mycotoxin produced by the metabolism of Rice false smut. Studies have shown that Ustiloxins may be toxic to animals, but there is still a lack of toxicological evidence. The liver, as the main organ for the biotransformation of foreign chemicals, may be the direct target organ of Ustiloxins toxicity.

Ultrasensitive Electrochemical Platform for Dopamine Detection Based on CoNi-MOF@ERGO Composite.

An electrochemical sensor applied for dopamine (DA) detection was constructed. An easy static way was used to synthesize bimetallic CoNi-MOF. Next, it was mixed with graphene oxide (GO) under ultrasound to get a uniform suspension.

Synthesis of a Red-Emitting Polarity-Sensitive Fluorescent Probe Based on ICT and Visualization for Lipid Droplet Dynamic Processes.

Abnormal lipid droplets (LDs) have been recognized as critical factors in many diseases because they are metabolically active and dynamic organelles. Visualization for LD dynamic processes is fundamental for elucidating the relationship of LDs and related diseases. Herein, a red-emitting polarity-sensitive fluorescent probe (TPA-CYP) based on intramolecular charge transfer (ICT) was proposed, which was constructed by employing triphenylamine (TPA) and 2-(5,5-dimethyl-2-cyclohex-1-ylidene)propanedinitrile (CYP) as electron donor and acceptor moiety, respectively.

Development of an mRNA vaccine against a panel of heterologous H1N1 seasonal influenza viruses using a consensus hemagglutinin sequence.

Seasonal influenza, causes hundreds of thousands of deaths annually, posing a severe threat to human health. Currently available influenza vaccines are targeted only at specific strains or conserved epitopes; however, these vaccines are not completely efficacious because influenza viruses can undergo mutation during circulation, leading to antigenic mismatch between recommended strains and circulating strains and elusion from the immune system. Therefore, developing an influenza vaccine that is quick, effective, and broadly protective has become crucial, and the integral part of hemagglutinin (HA) remains an ideal target for vaccine development.

Type XV osteogenesis imperfecta: A novel mutation in the gene, c.620G >A (p.R207H), is associated with an inner ear deformity.

The Wnt signaling pathway is vital in encouraging bone growth. gene mutations have been identified as the major cause of type XV osteogenesis imperfecta (OI). Described here is a case of complex heterozygous c.

Automated machine learning-based model for the prediction of delirium in patients after surgery for degenerative spinal disease.

Objective: This study used machine learning algorithms to identify critical variables and predict postoperative delirium (POD) in patients with degenerative spinal disease.

Methods: We included 663 patients who underwent surgery for degenerative spinal disease and received general anesthesia. The LASSO method was used to screen essential features associated with POD.

The Characteristics and Potential Risks of Neonicotinoid Residues in Soils of Different Types of Land Use in Hangzhou.

Due to the wide existence of neonicotinoid insecticides (neonics) and their potential impact on ecosystems and human health, they have received special attention in recent years. Soil is not only a sink of neonics but also a source of neonics, so it plays a key role in the ubiquity of neonics in the environment. The purpose of this research was to compare neonics residues in soils of different types of land use and estimate their exposure to different populations via ingestion.

Preparation of Nereid oligopeptide and investigation of the mechanism underlying the induction of apoptosis in human lung cancer H1299 cells.

In the present study, oligopeptides from Nereid () were prepared via enzymatic hydrolysis, and the mechanism underlying the induction of apoptosis in H1299 cells was investigated. According to the analysis of the inhibition rate on proliferation, alkaline protease demonstrated the best enzymatic efficiency. The optimal conditions for hydrolysis were as follows: 50˚C and pH 10 for 6 h; a material‑to‑liquid ratio of 1:1 (g/ml); and addition of 400 U/g enzyme.

TICT-Based Microenvironment-Sensitive Probe with Turn-on Red Emission for Human Serum Albumin Detection and for Targeting Lipid Droplet Imaging.

Fluorescent probes sensitive to microenvironment have always been fascinating due to their tremendous advantages in tracking changes in the pathophysiological microenvironment and potential application in the early diagnosis of related diseases. In this study, a fluorescent luminogen, triphenylamine-thiophene-rhodanine (TPA-TRDN), with high sensitivity to changes in polarity and viscosity was designed and could be applied to detecting human serum albumin (HSA) in actual urine, as well as lipid droplets (LDs) in cells and in vivo with turn-on red emission. TPA-TRDN could selectively detect HSA with fast response (10 min), superior sensitivity (LOD 0.

Synthesis of a new environment-sensitive fluorescent probe based on TICT and application for detection of human serum albumin and specific lipid droplets imaging.

Environment-sensitive fluorescent probes have always been as forceful tools to understand the pathophysiological processes of relevant diseases. In this work, a new fluorescent probe with typical D-π-A structure was designed and showed high sensitivity to polarity and viscosity changes. DPAR could selectively detect human serum albumin (HSA) with turn-on orange emission in aqueous PBS buffer (pH 7.

Lysosome targeting, Cr(vi) and l-AA sensing, and cell imaging based on N-doped blue-fluorescence carbon dots.

N-doped blue-fluorescence carbon dots (N-CDs) were fabricated via a one-pot hydrothermal method using folic acid and p-phenylenediamine. The obtained N-CDs exhibited strong fluorescence (FL) with a considerable quantum yield (QY) of 21.8% and exceptional optical stability under different conditions.

Good's buffers have various affinities to gold nanoparticles regulating fluorescent and colorimetric DNA sensing.

Citrate-capped gold nanoparticles (AuNPs) are highly important for sensing, drug delivery, and materials design. Many of their reactions take place in various buffers such as phosphate and Good's buffers. The effect of buffer on the surface properties of AuNPs is critical, yet this topic has not been systematically explored.

Development of a piperazinyl-NBD-based fluorescent probe and its dual-channel detection for hydrogen sulfide.

To selectively detect HS based on the thiolysis reaction of 7-nitro-1,2,3-benzoxadiazole (NBD), amines attracted increasing attention since NBD amine is regarded as a new HS reaction site. Herein, a novel fluorescent probe, triphenylamine piperazine NBD (TPA-Pz-NBD), was developed. The results showed that it exhibited high selectivity towards HS via fluorescence spectroscopy and solution color.

Orange emissive carbon nanodots for fluorescent and colorimetric bimodal discrimination of Cu and pH.

We have facilely synthesized orange emissive carbon nanodots (O-CDs) via a hydrothermal method using citric acid and 5-aminosalicylic acid. The obtained O-CDs show the excellent characteristics of excitation independence, low toxicity, fabulous photostability and superior biocompatibility. Based on these captivating properties, as-prepared O-CDs have been successfully implemented as a multi-functional sensing platform for fluorescent and colorimetric bimodal recognition of Cu and pH.

Silk Fibroin-Confined Star-Shaped Decahedral Silver Nanoparticles as Fluorescent Probe for Detection of Cu and Pyrophosphate.

A green, one-step method for the fabrication of silk fibroin (SF) protected silver decahedral nanoparticles (SF@AgNPs) has been developed. High-resolution transmission electron microscopy characterization demonstrated that the silver decahedral nanoparticles can provide more binding sites with (111) facets. Moreover, a facile strategy based on Cu mediated SF@AgNPs was reported as an on-off-on fluorescent system for the detection of Cu, and the SF@AgNPs can be also used for the determination of pyrophosphate ion (PO, PPi).

A red emitting fluorescent probe based on TICT for selective detection and imaging of HSA.

A red emitting fluorescence probe, TPA-CPO, based on twisted intra-molecular charge transfer (TICT) was designed and synthesized. The spectra results displayed that TPA-CPO could sense HSA with excellent properties including significant fluorescence enhancement, long emission wavelength, large stokes shift, and wide linear range. The recognition mechanism was proved that TPA-CPO could bind to domain IB of HSA and its TICT process was suppressed by utilizing hydrophobic cavity and low polarity of HSA.

Synergistic Effects of Ternary PdO-CeO-OMS-2 Catalyst Afford High Catalytic Performance and Stability in the Reduction of NO with CO.

We developed a robust ternary PdO-CeO-OMS-2 catalyst with excellent catalytic performance in the selective reduction of NO with CO using a strategy based on combining components that synergistically interact leading to an effective abatement of these toxic gases. The catalyst affords 100% selectivity to N at the nearly full conversion of NO and CO at 250 °C, high stability in the presence of HO, and a remarkable SO tolerance. To unravel the origin of the excellent catalytic performance, the structural and chemical properties of the PdO-CeO-OMS-2 nanocomposite were analyzed in the as-prepared and used state of the catalyst, employing a series of pertinent characterization methods and specific catalytic tests.

A turn-on fluorescence probe for hydrogen sulfide in absolute aqueous solution.

A turn-on hydrogen sulfide (HS) fluorescence probe, 4-{2-[4-(2,4-dinitrophenoxy)-phenyl]-vinyl}-1-methyl-pyridinium iodide (DPPVP), based on the thiolysis reaction of dinitrophenyl ethers (DNP) has been proposed. Pyridinium structure enhanced the water solubility of DPPVP, which could quickly respond to HS in absolute PBS solution and the fluorescence spectra of DPPVP at 520 nm were turned on by HS. The spectra results exhibited that DPPVP could sensitively detect HS with satisfied linear range (0-40 μM) and detection limit (13.

Smilax China-derived yellow-fluorescent carbon dots for temperature sensing, Cu detection and cell imaging.

Here, we report an environmentally friendly fabrication strategy of bright yellow fluorescent carbon dots (y-CDs) and construct a rapid and accurate multifunctional sensing platform for the effective detection of temperature and Cu2+. The y-CDs were favorably obtained through a one-step hydrothermal treatment of natural Smilax China for the first time and exhibit long-wavelength emission at 542 nm under an excitation wavelength of 470 nm. Additionally, the obtained y-CDs exhibit superior biocompatibility and distinguished stability under different conditions, and display a respectable fluorescence quantum yield of up to 22.

A novel cell-penetrating Janus nanoprobe for ratiometric fluorescence detection of pH in living cells.

pH regulates the function of many organelles and plays a pivotal role in requiring multitud cellular behaviors. Compared with single fluorescent probes, ratio fluorescent probes have higher sensitivity and immunity to interference. Herein, a novel Janus ratio nanoprobe was developed for intracellular pH detection.