TMT-label comparative proteomics reveals the vernalization mechanism in Wucai (Brassica campestris L.).

To investigate the molecular basis of vernalization in Wucai [Brassica campestris L. (Syn. Brassica rapa L.

Genome-Wide Identification and Expression Analysis of bHLH-MYC Family Genes from Mustard That May Be Important in Trichome Formation.

The trichomes of mustard leaves have significance due to their ability to combat unfavorable external conditions and enhance disease resistance. It was demonstrated that the MYB-bHLH-WD40 (MBW) ternary complex consists of MYB, basic Helix-Loop-Helix (bHLH), and WD40-repeat (WD40) family proteins and plays a key role in regulating trichome formation and density. The bHLH gene family, particularly the Myelocytomatosis (MYC) proteins that possess the structural bHLH domain (termed bHLH-MYC), are crucial to the formation and development of leaf trichomes in plants.

Modulating the structure of O3-type NaNiFeMnO for high-performance sodium-ion batteries via NaMoO reactive wetting coating combined with Mo doping and interface reconstruction.

O3-type NaNiFeMnO (NFM) is considered as a promising cathode material for sodium-ion batteries (SIBs) due to its high theoretical energy density and low production cost. However, the applications of NFM are restricted owing to detrimental interfacial side reactions and phase evolution during cycling. Herein, a three-in-one modification strategy, including NaMoO coating, surface reconstruction from layered to spinel phase, and Mo doping, is proposed to design NFM.

Enhancing Environment Resistance and Bioactivity of Pesticide Enabled by Structure-Controllable Polymer Nanocarriers: Emphasizing the Role of Morphology.

Nano-formulated pesticides are increasingly desired to control the insect pest and plant disease with superior efficacy for guaranteeing the high yield and quality in crop production. However, the impact of nanocarrier morphology on pesticide resistance against rainwash, photolysis, and overall pesticide bioactivity remains unknown. In this work, a series of well-defined and morphology-controllable polymer nanocarriers for pesticide are fabricated through polymerization-induced self-assembly.

Comparative Morphological, Physiological, and Transcriptomic Analyses of Diploid and Tetraploid Wucai ( L.).

Polyploid plants often exhibit superior yield, stress resistance, and quality. In this study, homologous tetraploid wucai ( L.) was successfully obtained by spraying seedling growth points with colchicine.

Exogenous spraying of IAA improved the efficiency of microspore embryogenesis in Wucai (Brassica campestris L.) by affecting the balance of endogenous hormones, energy metabolism, and cell wall degradation.

Background: Microspore embryogenesis is an extraordinarily complicated process, comprehensively regulated by a composite network of physiological and molecular factors, among which hormone is one of the most crucial factors. Auxin is required for stress-induced microspore reprogramming, however, the mechanism of its regulation of microspore embryogenesis is still unclear.

Results: In this study, we found exogenously spraying 100 mg·L IAA on the buds of Wucai significantly increased the rate of microspore embryogenesis, and moreover accelerated the process of embryogenesis.

Auxin participates in regulating the leaf curl development of Wucai (Brassica campestris L.).

Wucai (Brassica campestris L. ssp. chinensis var.

Combined Metabolome and Transcriptome Analysis Elucidates Sugar Accumulation in Wucai ( L.).

Wucai ( L.) is a leafy vegetable that originated in China, its soluble sugars accumulate significantly to improve taste quality during maturation, and it is widely accepted by consumers. In this study, we investigated the soluble sugar content at different developmental stages.

Identification of the Gene Family and Functional Analysis of the Gene in Wucai ( L.).

Late embryogenesis abundant (LEA) proteins are important developmental proteins in the response of plants to abiotic stress. In our previous study, was differentially expressed under low-temperature stress. Herein, we combined bioinformatics analysis, subcellular localization, expression assays, and stress experiments (including salt, drought, and osmotic stress) to identify and analyze the BcLEA gene family.

Mapping and Validation of : A Dominant Gene Regulating Carotenoid Accumulation in .

In plants, the accumulation of carotenoids can maintain the balance of the photosystem and improve crop nutritional quality. Therefore, the molecular mechanisms underlying carotenoid synthesis and accumulation should be further explored. In this study, carotenoid accumulation differed significantly among parental .

Genome-wide analysis of the DREB family genes and functional identification of the involvement of BrDREB2B in abiotic stress in wucai (Brassica campestris L.).

Dehydration responsive element binding protein (DREB) is a significant transcription factor class known to be implicated in abiotic stresses. In this study, we systematically conducted a genome-wide identification and expression analysis of the DREB gene family, including gene structures, evolutionary relationships, chromosome distribution, conserved domains, and expression patterns. A total of 65 DREB family gene members were identified in Chinese cabbage (Brassica rapa L.

Heat stress response in Chinese cabbage ( L.) revealed by transcriptome and physiological analysis.

High temperatures have a serious impact on the quality and yield of cold-loving Chinese cabbage, which has evolved to have a unique set of stress mechanisms. To explore the relationship between these mechanisms and the heat-tolerance of Chinese cabbage, the physiological indicators of the heat-tolerant '268' line and heat-sensitive '334' line were measured. Under heat stress, the proline (Pro), soluble sugar (SS), and superoxide dismutase (SOD) indexes of the '268' line increased significantly.

Transcriptome analysis and differential gene expression profiling of wucai (Brassica campestris L.) in response to cold stress.

Background: Wucai suffers from low temperature during the growth period, resulting in a decline in yield and poor quality. But the molecular mechanisms of cold tolerance in wucai are still unclear.

Results: According to the phenotypes and physiological indexes, we screened out the cold-tolerant genotype "W18" (named CT) and cold-sensitive genotype "Sw-1" (named CS) in six wucai genotypes.

TMT-based comparative proteomic analysis of the male-sterile mutant ms01 sheds light on sporopollenin production and pollen development in wucai (Brassica campestris L.).

A spontaneous male-sterile mutant ms01 was discovered from the excellent high-generation inbred line 'hx12-6-3' in wucai. Compared with wild-type 'hx12-6-3', ms01 displayed complete male sterility with degenerated stamens and no pollen. In this study, cytological observation revealed that the tapetum of the anthers of ms01 had degraded in advance, and microspore development had stagnated in the mononuclear stage, ultimately resulting in completely aborted pollen.

Comparative transcriptome analysis reveals that chlorophyll metabolism contributes to leaf color changes in wucai (Brassica campestris L.) in response to cold.

Background: Chlorophyll (Chl) is a vital photosynthetic pigment involved in capturing light energy and energy conversion. In this study, the color conversion of inner-leaves from green to yellow in the new wucai (Brassica campestris L.) cultivar W7-2 was detected under low temperature.

Transcriptional profiling reveals changes in gene regulation and signaling transduction pathways during temperature stress in wucai (Brassica campestris L.).

Background: Wucai (Brassica campestris L. ssp. chinensis var.

Morphological characteristics and transcriptome analysis at different anther development stages of the male sterile mutant MS7-2 in Wucai (Brassica campestris L.).

Background: The discovery of male sterile materials is of great significance for the development of plant fertility research. Wucai (Brassica campestris L. ssp.

Characterization and transcriptomic analysis of a novel yellow-green leaf wucai (Brassica campestris L.) germplasm.

Background: Leaf color mutants are the ideal materials to explore the pathways of chlorophyll (Chl) metabolism, chloroplast development, and photosynthesis system. In this study, a spontaneous yellow-green leaf wucai (Brassica campestris L.) mutant "WY16-13" was identified, which exhibited yellow-green leaf color during its entire growth period.

Gene co-expression network analysis reveals key pathways and hub genes in Chinese cabbage (Brassica rapa L.) during vernalization.

Background: Vernalization is a type of low temperature stress used to promote rapid bolting and flowering in plants. Although rapid bolting and flowering promote the reproduction of Chinese cabbages (Brassica rapa L. ssp.

The effect of exogenous 24-epibrassinolide pretreatment on the quality, antioxidant capacity, and postharvest life of wucai ( L.).

The quality of green leafy vegetables is easily lost during the postharvest period. The effect of exogenous 24-epibrassinolide (EBR) pretreatment on the quality of wucai was evaluated in the present study. Wucai plants were sprayed twice with 0.

Transcriptome Analysis Reveals the Symbiotic Mechanism of -Induced Gall Formation of .

is a perennial aquatic vegetable, whose symbiosis with the fungus (member of Basidiomycota, class ) results in the establishment of swollen gall formations. Here, we analyzed symbiotic relations of and , using a triadimefon (TDF) treatment and transcriptome sequencing (RNA-seq). Specifically, accurately identify the whole growth cycle of Microstructure observations showed that the presence of could be clearly observed after gall formation but was absent after the TDF treatment.

Genome-wide analysis of proline-rich extension-like receptor protein kinase (PERK) in Brassica rapa and its association with the pollen development.

Background: Proline-rich extension-like receptor protein kinases (PERKs) are an important class of receptor kinases located in the plasma membrane, most of which play a vital role in pollen development.

Results: Our study identified 25 putative PERK genes from the whole Brassica rapa genome (AA). Phylogenetic analysis of PERK protein sequences from 16 Brassicaceae species divided them into four subfamilies.