CircABCC1 promotes the development of glioma by sponging miR-591 and modulating high-mobility group A2.

CircABCC1 plays an oncogenic role in diverse malignancies. In this study, we investigated its involvement in glioma. The expression of circABCC1 and miR-591 was detected in glioma tissues and cell lines.

ATP2B1-AS1 Promotes Cerebral Ischemia/Reperfusion Injury Through Regulating the miR-330-5p/TLR4-MyD88-NF-κB Signaling Pathway.

We aim to explore the expression and function of long non-coding RNA (lncRNA) ATP2B1-AS1 in a cerebral ischemia/reperfusion (I/R) injury. In this study, we established a middle cerebral artery occlusion/reperfusion (MCAO/IR) rat model and an OGD/R PC12 cell model to evaluate the expression and role of ATP2B1-AS1 in the cerebral I/R injury. We found that the expression of ATP2B1-AS1 was upregulated in both and cerebral I/R injury models.

Resveratrol inhibits β-amyloid-induced neuronal apoptosis via regulation of p53 acetylation in PC12 cells.

The natural product resveratrol possesses diverse biological activities, including anti‑inflammatory, anti‑oxidant, anti‑cancer and anti‑aging effects in multiple organisms. The neuroprotective role of resveratrol has recently been reported in a cell model of amyloid (A)β(25‑35)‑induced neurotoxic injury using PC12 cells. However, the pathomechanism by which resveratrol inhibits neuronal apoptosis has remained to be elucidated.

The comparison of EPC count and function in the situation of vascular repair at early and late stage.

To investigate the relationship between the changes in the number and function of the late endothelial progenitor cells (EPC) in peripheral blood and the carotid artery stenosis. 60 cases were selected and were divided into the carotid artery stenosis group of 40 cases (mild stenosis in 20 cases, moderate/severe stenosis in 20 cases), normal control group of 20 cases with the global cerebral angiography. Extracted the blood of femoral artery from the patients during the global cerebral angiography, mononuclear cells were isolated from peripheral blood by density-gradient centrifugation and were cultured to 21 days when they were identified as late endothelial progenitor cells, counted the colony numbers of late EPC.

[Circumcision with the Chinese Shang Ring in children: outcomes of 824 cases].

Objective: To observe the clinical effects of using the Chinese Shang Ring in circumcision children with either phimosis or redundant prepuce, and to investigate its superiority over the similar devices available.

Methods: A total of 824 children with phimosis or redundant prepuce underwent circumcision with the Shang Ring. The clinical data were assessed concerning the duration of the procedure, incidence of post-operative complications, time of recovery and appearance of the penis.

[A historic cohort study on the survival rate and the correlated risk factors regarding 174 patients with subarachnoid hemorrhage].

Objective: To describe the survival state and to investigate the risk factors of death on patients with subarachnoid hemorrhage (SAH).

Methods: Age, past history, number of encephalic region suffering SAH, laboratory examination indexes, therapeutic measures, complications and prognosis of 174 patients with SAH were followed-up and investigated. The survival states and risk factors of death of the patients with SAH were identified by both Kaplan-Meier survival analysis and Cox proportional risk model.

Nerve-pathways of acupoint Fengch'ih in rat by anterograde transport of HRP.

Aim: To study the nervous-pathways of Fengch'ih acupuncture by means of anterograde transport of aqueous solution of horseradish peroxidase (HRP).

Methods: Fifty Wistar rats were randomly divided into 1, 2, 3, 4, and 5 d groups, and every group had 10 animals. HRP (30% aqueous solution) was injected into a Fengch'ih.

[In vitro induction and expansion of dendritic cells from rat bone marrow and their characterization].

Aim: To induce and expand dendritic cells (DC) from rat bone marrow in vitro and identify their biological characterization.

Methods: The rat bone marrow cells were collected and cultured for 48 hours and the floating cells were removed. Then IL-4 and GM-CSF were added into the fresh medium.

Evaluation of murine models of permanent focal cerebral ischemia.

Background: To date murine models of permanent focal cerebral ischemia have not been well characterized. The purposes of this paper were to compare three different permanent middle cerebral artery occlusion (MCAo) models with or without craniectomy, and to identify an ideal mouse model of permanent focal cerebral ischemia.

Methods: Experiments were performed on 45 healthy adult male Kunming mice, weighing 28 to 42 g.