Research of restricted migration evaluation of MDA-MB-231 cells in 2D and 3D co-culture models.

The restricted migration evaluation is conducive to more complex tumor migration research because of the conformity with tumors. However, the differences between restricted and unrestricted cell migration and the distinction between different evaluation methods have not been systematically studied, hindering related research. In this study, by constructing the restricted environments on chips, the influence of co-culture conditions on the cancer cell migration capacity was studied.

Investigation of the Solar Hydrogen Sensitivity of GeSe Thin Film Photoelectrode with Photoelectrochemical Environment.

GeSe photovoltaic thin films are very promising for photoelectrochemical (PEC) hydrogen evolution. The GeSe-based PEC water splitting device is a system containing a photoelectrode, electrolyte, and other packages, and the performance of the GeSe photoelectrode inside the system is very sensitive to the PEC system environment, such as the electrolyte temperature, pH, and concentration. Here, we reveal how the electrolyte environment at the electrolyte/photoelectrode interface influences the optoelectronic/PEC properties of GeSe photoelectrodes.

A comprehensively prognostic and immunological analysis of chloride intracellular channel protein 5 (CLIC5) in pan-cancer and identification in ovarian cancer.

CLIC5 encoded protein associates with actin-based cytoskeletal and is increasingly thought to play significant roles in human cancers. We use TCGA and GEO to explore CLIC5 expression differences, mutation and DNA methylation, TMB, MSI, and immune cell infiltration. We verified the mRNA expression of CLIC5 in human ovarian cancer cells by real-time PCR and detected the expression of CLIC5 as well as immune marker genes in ovarian cancer by immunohistochemistry.

Prognostic signature construction and immunotherapy response analysis for Uterine Corpus Endometrial Carcinoma based on cuproptosis-related lncRNAs.

Background: As a general female malignant tumor, Uterine Corpus Endometrial Carcinoma (UCEC) has high mortality and relapses. Cuproptosis was found to play an essential role in tumor by more and more researches. However, it is still unclear of the prognostic value and function of cuproptosis related Long non-coding RNA (lncRNA) in UCEC.

Process Accumulated 8% Efficient Cu ZnSnS -BiVO Tandem Cell for Solar Hydrogen Evolution with the Dynamic Balance of Solar Energy Storage and Conversion.

A process accumulated record solar to hydrogen (STH) conversion efficiency of 8% is achieved on the Cu ZnSnS -BiVO tandem cell by the synergistic coupling effect of solar thermal and photoelectrochemical (PEC) water splitting with the dynamic balance of solar energy storage and conversion of the greenhouse system. This is the first report of a Cu ZnSnS -BiVO tandem cell with a high unbiased STH efficiency of over 8% for solar water splitting due to the greenhouse device system. The greenhouse acts as a solar thermal energy storage cell, which absorbs infrared solar light and storage as thermal energy with the solar light illumination time, while thermoelectric device (TD) converts thermal energy into electric power, electric power is also recycled and added onto Cu ZnSnS -BiVO tandem cell for enhanced overall water splitting.

Research on the Influence of the Interfacial Properties Between a Cu BiS Film and an In Cd S Buffer Layer for Photoelectrochemical Water Splitting.

The ternary compound photovoltaic semiconductor Cu BiS thin film-based photoelectrode demonstrates a quite promising potential for photoelectrochemical hydrogen evolution. The presented high onset potential of 0.9 V attracts much attention and shows that the Cu BiS thin films are quite good as an efficient solar water splitting photoelectrode.

Changes in the gut microbiota during Asian particolored bat () development.

Background: The gut microbiota is closely linked to host development, diet and health and is influenced by both the host and the environment. Although many studies have focused on the dynamics of the gut microbiota during development in captive animals, few studies have focused on the dynamics of the gut microbiota during development in wild animals, especially for the order Chiroptera.

Methods: In this study, we characterized the gut microbiota of the wild Asian particolored bat () from 1 day to 6 weeks after birth.

A study of entity-linking methods for normalizing Chinese diagnosis and procedure terms to ICD codes.

Objective: This study aims to develop and evaluate effective methods that can normalize diagnosis and procedure terms written by physicians to standard concepts in International Classification of Diseases(ICD) in Chinese, with the goal to facilitate automated medical coding in China.

Methods: We applied the entity-linking framework to normalize Chinese diagnosis and procedure terms, which consists of two steps - candidate concept generation and candidate concept ranking. For candidate concept generation, we implemented both the traditional BM25 algorithm and an extended version that integrates a synonym knowledgebase.

Seasonal Changes in Gut Microbiota Diversity and Composition in the Greater Horseshoe Bat.

A large number of microorganisms colonize the intestines of animals. The gut microbiota plays an important role in nutrient metabolism and affects a number of physiological mechanisms in the host. Studies have shown that seasonal changes occur in the intestinal microbes of mammals that hibernate seasonally.

Captivity causes taxonomic and functional convergence of gut microbial communities in bats.

Background: Diet plays a crucial role in sculpting microbial communities. Similar diets appear to drive convergence of gut microbial communities between host species. Captivity usually provides an identical diet and environment to different animal species that normally have similar diets.

Clinical value screening, prognostic significance and key pathway identification of miR-204-5p in endometrial carcinoma: A study based on the Cancer Genome Atlas (TCGA), and bioinformatics analysis.

Background: Endometrial carcinoma is one of the common carcinomas in the female reproductive system. It is reported that miR-204-5p is down-regulated in endometrial carcinoma. However, the mechanism and key pathways of miR-204-5p in endometrial carcinoma have not been clarified.

Metformin combined with nelfinavir induces SIRT3/mROS-dependent autophagy in human cervical cancer cells and xenograft in nude mice.

The molecular mechanisms underlying the antineoplastic properties of metformin combined with nelfinavir remain elusive. To explore this question, transmission electron microscopy (TEM) was used to observe the combinatorial effect of inducing autophagosome formation in human cervical cancer cells. Western blotting respectively assayed protein expression of LC3I, LC3II, Beclin-1, Autophagy-related protein 7 (Atg7), Autophagy-related protein 3 (Atg3), NAD-dependent deacetylase sirtuin-3 (SIRT3) and major histocompatibility complex class I chain-related gene A (MICA).

Re-recognition of the principle of ultrasonic reverberation image artifacts.

The aim of this work was to describe the physical principles behind the reverberation artifacts in ultrasound imaging and list the correct interpretation. This work focuses on the understanding of the principle of reverberation imaging artifacts.

Combining metformin and nelfinavir exhibits synergistic effects against the growth of human cervical cancer cells and xenograft in nude mice.

Human cervical cancer is the fourth most common carcinoma in women worldwide. However, the emergence of drug resistance calls for continuously developing new anticancer drugs and combination chemotherapy regimens. The present study aimed to investigate the anti-cervical cancer effects of metformin, a first-line therapeutic drug for type 2 diabetes mellitus, and nelfinavir, an HIV protease inhibitor, when used alone or in combination.

Disrupting MALAT1/miR-200c sponge decreases invasion and migration in endometrioid endometrial carcinoma.

Endometrioid endometrial carcinoma (EEC) is the most common gynecologic malignancy around the world. Epithelial-to-mesenchymal transition (EMT) is a core process during EEC cell invasion. The abnormal expression of the long noncoding RNA metastasis associated lung adenocarcinoma transcript 1 (MALAT1) or miR-200 family members were shown to facilitate EMT in multiple human cancers, but the regulatory mechanism by which MALAT1 and miR-200 act remains unknown.

Curcumin Increases HSP70 Expression in Primary Rat Cortical Neuronal Apoptosis Induced by gp120 V3 Loop Peptide.

Neuronal cell dysfunction and apoptosis are the main causes of the invasion of the central nervous system by human immunodeficiency virus type 1 (HIV-1), although the underlying mechanism has not been well understood. Recent research has shown that curcumin might play an important role in regulating HIV-1 development. Heat shock protein 70 (HSP70), a protein induced by heat, was reported to inhibit apoptosis through various cell signaling pathways in brain.

MiR-133b-5p regulates the expression of the heat shock protein 70 during rat neuronal cell apoptosis induced by the gp120 V3 loop peptide.

Neuronal cell dysfunction and apoptosis, the main causes of HIV-associated dementia, and its underlying mechanism are important unsolved health problems. Many research reports suggest that miRNAs regulate HIV-1-induced apoptosis. We used the HIV-1 gp120 V3 Loop peptide to induce primary rat cortical neurons apoptosis.

[Placental Foxp3 expression in patients with preeclampsia and correlation of Foxp3 gene locus 924 (rs2232365) polymorphism with preeclampsia].

Objective: To detect changes of Foxp3 expression in the decidua in patients with preeclampsia and investigate the correlation of Foxp3-924 (rs2232365) polymorphisms with preeclampsia.

Methods: From October 2011 to December 2012, 252 normal pregnant women and 156 preeclampsia patients of Han nationality from the same geographic region were tested for Foxp3-924 genotypes by polymerase chain reaction with sequence-specific primer (PCR-SSP). Sixty-eight of the patients with preeclampsia (33 with mild and 35 with severe preeclampsia) and 30 of the normal pregnant women were also examined for Foxp3 expression in the decidua using immunohistochemical method.

Polymorphisms and haplotypes of the TGF-β1 gene are associated with risk of polycystic ovary syndrome in Chinese Han women.

Objectives: Transforming growth factor-beta 1 (TGF-β1) is one of the multifunctional cytokine families. It takes part in a series of physiological and pathological processes in the human body, including wound healing, tissue fibrosis and embryonic development. Recent studies have shown that TGF-β1 participates in the development of polycystic ovary syndrome (PCOS).

Integrated microRNA and mRNA transcriptome sequencing reveals the potential roles of miRNAs in stage I endometrioid endometrial carcinoma.

Endometrioid endometrial carcinoma (EEC) is the most dominant subtype of endometrial cancer. Aberrant transcriptional regulation has been implicated in EEC. Herein, we characterized mRNA and miRNA transcriptomes by RNA sequencing in EEC to investigate potential molecular mechanisms underlying the pathogenesis.

MiR-133b regulates the expression of the Actin protein TAGLN2 during oocyte growth and maturation: a potential target for infertility therapy.

Infertility is an area of increasing in life science research. Although follicular maturation disorders and anovulation are the primary causations of infertility, its molecular mechanism is not well understood. Recent research has shown that microRNAs (miRNAs) might play an important role in the regulation of ovarian follicle development and maturation.

[Mechanisms of foam cell formation in smooth muscle cell derived from bone marrow stem cells].

Objective: To establish a model of smooth muscle cells differentiated from bone mesenchymal stem cells (BMSC-SMCs) in vitro and explore the relationship between scavenger receptors A (SR-A) and caveolin-1.

Methods: BMSCs were isolated from the femoral bone of SD rats by adherent culture. After treatment of the BMSC-SMCs with 80 mg/L ox-LDL for 72 h, Western blotting was performed to detect the expression of scavenger receptor SR-A, cell cholesterol transport protein ATP-binding cassette transporter Al (ABCA1) and caveolin-1.

[Effect of oxidative low-density lipoprotein on the proliferation of bone marrow stem cell- derived smooth muscle cells].

Objective: To establish the model of bone mesenchymal stem cell-derived smooth muscle cells (BMSC-SMCs) and investigate the role of BMSC-SMCs in the development and progression of artherosclerosis.

Methods: BMSCs were isolated from the femoral bone of SD rats by adherent tissue culture method, and vascular smooth muscle cells (VSMCs) were obtained from the thoracic aorta. The differentiation of BMSCs into BMSC-SMCs was induced in the conditioned medium.

Derivation and characterization of human embryonic stem cell lines from poor quality embryos.

Poor quality embryos discarded from in vitro fertilization (IVF) laboratories are good sources for deriving human embryonic stem cell (hESC) lines. In this study, 166 poor quality embryos donated from IVF centers on day 3 were cultured in a blastocyst medium for 2 days, and 32 early blastocysts were further cultured in a blastocyst optimum culture medium for additional 2 days so that the inner cell masses (ICMs) could be identified and isolated easily. The ICMs of 17 blastocysts were isolated by a mechanical method, while those of the other 15 blastocysts were isolated by immunosurgery.