The combination of microplastics and glyphosate affects the microbiome of soil inhabitant Enchytraeus crypticus.

Microplastics and pesticides are emerging contaminants that threaten soil ecosystems, yet their combined effects on soil health and soil fauna remain poorly understood. In this study, we constructed a microcosm to assess the individual and combined effects of microplastics and glyphosate on soil physicochemical properties, microbial communities, and the gut microbiome of soil invertebrates (Enchytraeus crypticus). Biodegradable polylactic acid (PLA) and conventional polyethylene terephthalate (PET) were introduced at environmentally relevant concentrations.

Machine learning models reveal how polycyclic aromatic hydrocarbons influence environmental bacterial communities.

Polycyclic aromatic hydrocarbons (PAHs) are harmful and widespread pollutants in the environment, posing an ecological threat. However, exploring the influence of PAHs on environmental bacterial communities in different habitats (soil, water, and sediment) remains a major challenge. We collected and reanalyzed 1924 16S rRNA sequencing samples to determine the effects of PAHs on bacterial communities in different habitats and used machine learning to predict potential degrading bacteria.

[Enantiomeric separation and impurity determination of valaciclovir hydrochloride].

Objective: To determine the contents of L-enantiomer impurity in valaciclovir hydrochloride.

Methods: Valaciclovir enantiomers were separated and determined by using chiral high performance liquid chromatography. Chromatographic conditions were as follows:CROWNPAK(®) CR(+) chiral column (4 mm×150 mm, 5 μm), detection wavelength:254 nm, mobile phase:water-methanol-perchloric acid (19:1:0.

[Determination of enantiomeric impurity of timolol maleate in bulk substances and eye drops].

Objective: To determine the enantiomeric impurity contents of domestic timolol maleate in bulk drugs and eye drops.

Methods: Enantiomer impurity of timolol was assayed by chiral high performance liquid chromatography. The chromatographic conditions were as follows:chiralcel OD chiral column (4.

[Rapid characterization of impurities in the bulk drug of nifedipine by high performance liquid chromatography-quadrupole time of fight mass spectrometry].

Four impurities in the bulk drug of nifedipine were detected by means of high performance liquid chromatography coupled with quadrupole time of fight mass spectrometry (HPLC-QTOF MS). A Kromasil C18 HPLC column (250 mm x 4.6 mm, 5 microm) was used with methanol-water (60:40, v/v) as mobile phase.

[Study on determination of desloratadine in human serum and its pharmacokinetics by HPLC/MS].

Objective: To study the determination of desloratadine in human serum and its pharmacokinetics in healthy volunteers.

Methods: A single oral dose of 10 mg desloratadine was given to 18 healthy volunteers. The serum concentrations of desloratadine were determined by HPLC-MS assay.