Distinguishing the botanical origins of rare honey through untargeted metabolomics and machine learning interpreting flavonoid profiles.

Distinguishing the botanic origins of monofloral honey is the foremost concern in ensuring its authentication. In this work, an innovative, green, and comprehensive approach was developed to distinguish the botanic origins of four types of rare honey, and the strategy involved in the following aspects: Based on theoretical design, suitable natural deep eutectic solvent (NADES) was screened to extract flavonoids from honey samples; after NADES extracts were directly analyzed by high-resolution mass spectrometry, the discrimination models of monofloral honey were established by untargeted metabolomics combined with machine learning. Based on the comparison of various models, the Random Forest algorithm had higher prediction accuracy for four types of monofloral honey, and characteristic compounds for each rare monofloral honey were screened based on SHapley Additive exPlanations values.

Based on theoretical design simultaneous analysis of multiple neonicotinoid pesticides in beeswax by deep eutectic solvents extraction combined with UHPLC-MS/MS.

Beeswax, an FDA-approved component, has been extensively applied in feed, pharmaceutical, and food industries. The occurrence of neonicotinoid pesticides in beehive systems and their residues in beeswax have caused safety risks. Therefore, establishing a detection method for neonicotinoid pesticide residues in beeswax is crucial for ensuring its quality.

Dual-use ionization source for direct and sensitive detection of trace carbamates residue.

Directly determining trace compounds in actual sample by ambient ionization mass spectrometry (AIMS) has always been a challenge. Due to the excellent adsorption and desorption properties of nanomaterials, AIMS combined with nanomaterials can improve the adsorption and desorption properties of MS. In this work, we developed a molecularly imprinted graphene oxide coated steel sheet (MIGOCS) to be used as a solid phase extraction adsorbent.

Exploring formation of turanose in honey via stable isotope labelling and high-resolution mass spectrometry analysis.

Turanose, an isomer of sucrose, naturally exists in honey. Previous study indicated that turanose content increased gradually in acacia honey as honeybees brewed honey in the hive. However, it is unclear how turanose is generated in honey.

Exploring the Formation of Chemical Markers in Chaste Honey by Comparative Metabolomics: From Nectar to Mature Honey.

Identification of chemical markers is important to ensure the authenticity of monofloral honey; however, the formation of chemical markers in honey has received little attention. Herein, using comparative metabolomics, we first identified chemical markers in chaste honey and then explored their formation and accumulation from nectar to mature honey. We identified agnuside and -hydroxybenzoic acid glucosides as chemical markers for chaste honey.

Chaste honey in long term-storage: Occurrence and accumulation of Maillard reaction products, and safety assessment.

Honey, a natural sweetener that can be stored long-term, is prone to Maillard reactions. Maillard reaction products (MRPs), such as 5-hydroxymethylfurfural (5-HMF), α-dicarbonyl compounds (α-DCs), and advanced glycation end products (AGEs), negatively affect human health. We analyzed MRP accumulation in chaste honey over four years.

Hydrophilic Covalent Organic Frameworks Coated Steel Sheet As a Mass Spectrometric Ionization Source for the Direct Determination of Zearalenone and Its Derivatives.

Zearalenone has attracted worldwide attention due to its toxic properties and threat to public health. A rapid determination method for zearalenone and its derivatives by hydrophilic covalent organic frameworks coated steel sheet (HCOFCS) combined with ambient mass spectrometry (AMS) was developed. The HCOFCS behaved as both a tip for solid-phase microextraction and a solid substrate for electrospray ionization mass spectrometry (ESI-MS).

Research Progress on Fumonisin B1 Contamination and Toxicity: A Review.

Fumonisin B1 (FB1), belonging to the member of fumonisins, is one of the most toxic mycotoxins produced mainly by and . FB1 has caused extensive contamination worldwide, mainly in corn, rice, wheat, and their products, while it also poses a health risk and is toxic to animals and human. It has been shown to cause oxidative stress, endoplasmic reticulum stress, cellular autophagy, and apoptosis.

Fast construction of core-shell structured magnetic covalent organic framework as sorbent for solid-phase extraction of zearalenone and its derivatives prior to their determination by UHPLC-MS/MS.

Magnetic covalent organic framework nanocomposite denoted as FeO@TAPB-Tp with core-shell structure was fabricated via a simple template-mediated precipitation polymerization method at mild conditions. The polyimine network shell was created through the polymerization of 1,3,5-tris(4-aminophenyl)-benzene (TAPB) and 1,3,5-triformyl-phloroglucinol (Tp) in tetrahydrofuran (THF) by the Schiff-base reaction. Featuring with large specific surface area (163.

Sheep as a Potential Source of Zoonotic Cryptosporidiosis in China.

In this study, we assessed the prevalence and genetic characteristics of in sheep from 10 provinces in China. Fecal samples from 1,035 sheep originating from 16 farms were collected, and 295 (28.5%) were found to be positive by nested PCR.

Novel influenza A(H7N2) virus in chickens, Jilin province, China, 2014.

In February 2014, while investigating the source of a human infection with influenza A(H7N9) virus in northern China, we isolated subtypes H7N2 and H9N2 viruses from chickens on the patient's farm. Sequence analysis revealed that the H7N2 virus is a novel reassortant of H7N9 and H9N2 viruses. Continued surveillance is needed.

Prevalence and genotype of Toxoplasma gondii infection in cattle from Jilin Province, northeastern China.

Abstract The prevalence and genotype of Toxoplasma gondii infection in cattle in Jilin Province, northeastern China was investigated. A total of 1040 serum samples were collected from eight administrative regions from September to October, 2011, and antibodies to T. gondii were examined by enzyme-linked immunosorbent assay (ELISA).