(Podostemaceae), a new species from Guangdong, South China, redefining the phylogenetic relationships within .

This paper introduces , a newly identified species that enriches our understanding of the diversity of the Podostemaceae in East Asia. Distinctive in its morphological traits, this species is characterized by the region's longest flowering shoots and exhibits a high number of elongated leaves per cluster, along with relatively slender roots. Phylogenetic analyses using Maximum Likelihood and Bayesian Inference methods on plastome and sequences confirm as a distinct species.

Fractionated Radiation Therapy for Large and Giant Cavernous Sinus Hemangioma: A Retrospective Study.

Surgical resection has been traditionally used as a treatment for cavernous sinus hemangioma (CSH). However, this is usually difficult due to tumor vascularity and results in complications especially in large and giant CSH (volume >20 cm). Previous studies have reported that radiotherapy (RT) provides an alternative treatment modality for hemangiomas.

Hypofractionated stereotactic radiation therapy activates the peripheral immune response in operable stage I non-small-cell lung cancer.

It has been reported that in patients with operable stage I non-small cell lung cancer (NSCLC), overall survival (OS) is better in those who undergo hypofractionated stereotactic radiation therapy (HSRT) than in those who undergo surgery. However, the reason that HSRT has a better OS has not been fully explored. Here, we analyzed reconstitution kinetics in immune cells in the peripheral blood of NSCLC patients after HSRT.

Ionizing radiation-induced microRNA expression changes in cultured RGC-5 cells.

MicroRNAs (miRNAs) are a class of short non-coding RNAs that regulate gene expression at the post‑transcriptional level. It has been demonstrated that miRNAs serve a crucial role in tissue development and the pathogenesis of numerous diseases. The aim of the current study was to investigate the alterations in miRNA expression in a cultured retinal ganglion cell line (RGC‑5 cells) following ionizing radiation injury.

The impact of matrix metalloproteinase 2 on prognosis and clinicopathology of breast cancer patients: a systematic meta-analysis.

Backgrounds: Matrix metalloproteinase 2 (MMP-2) plays a crucial role in the progression of breast cancer (BC). The prognostic role of MMP-2 expression in BC patients has been widely reported, but the results were inconsistent. Thus, a meta-analysis was conducted to gain a better insight into the impact of MMP-2 expression on survival and clinicopathological features of BC patients.

Meta-analysis demonstrates lack of association of the GSK3B -50C/T polymorphism with risk of bipolar disorder.

Published data on the association between GSK3B -50C/T (rs334558) and bipolar disorder (BD) are inconclusive. We performed this meta-analysis to evaluate the relationship of this single-nucleotide polymorphism with the susceptibility, and with the age at onset of BD. A literature search was conducted though PubMed, EMBASE, Web of Science and China National Knowledge Infrastructure databases to identify relevant studies up to February 14, 2014.

Lack of association between MDR1 C3435T polymorphism and chemotherapy response in advanced breast cancer patients: evidence from current studies.

The transmembrane transport of anticancer drugs is mainly regulated by P-glycoprotein encoded by the human multidrug resistance gene 1 gene (MDR1). Since there were controversies regarding the association between MDR1 C3435T polymorphism and response to chemotherapy among patients with advanced breast cancer, a meta-analysis of the link was conducted. A total of 7 studies consist of 464 advanced breast cancer patients relating MDR1 C3435T polymorphism to the response of chemotherapy were included in this meta-analysis.

[Multiplex PCR and genetic polymorphism of STR loci D4S1627, D14S1426, D1S1649].

Objective: To prepare the allelic ladder materials of three STR loci includes D1S1649, D4S1627, D14S1426, and construct a synchronous detection method by multiplex amplification of the three loci.

Methods: Multiplex PCR method, PAGE (polyacrylamide gel electrophoresis) and silver staining were applied to detect 120 unrelated Chinese Han population from Chengdu whose allele and gene frequency distributing of these three loci. The electrophoretic bands of the multiplex PCR of these three loci were clear, no overlap of each alleles were observed, and the results were the same with single-locus test results.

[The expression of BDNF and PSD-95 in hippocampal CA1 region of morphine-withdrawn rat with different dependent times].

Objective: To observe the expression of brain-derived neurotrophic factor (BDNF) and postsynaptic density-95 (PSD-95) in hippocampal CA1 region of rat with morphine dependence for different times and withdrawn for 1 week, and investigate the influence of that morphine dependence is withdrawn on rat hippocampal CA1 area.

Methods: Animal models of rats with morphine withdrawal for 1 week and different morphine dependent times(1 week, 2 weeks, 4 weeks) were established. The expression of BDNF and PSD-95 in hippocampal CA1 were identified with RT-PCR.

[Polymorphism of five short tandem repeat systems in Chinese Han population in Chengdu].

Objective: To obtain population genetic data of loci D11S4951, D11S4957, GATA193H05, D2S2951, and D6S2421 in Han population in Chengdu area and to validate the value of their forensic application.

Methods: Blood samples were collected in EDTA tubes from unrelated individuals. DNAs were extracted with Chelex-100 and were analyzed by PCR and horizontal PAGE followed by silver staining.

[Genetic polymorphisms of three loci and implication to forensic medicine].

Objective: To obtain population genetic data of short tandem repeat (STR) locus D2S1327, D1S1390, and D11S2008 and to investigate the disparity of allelic frequency distributions among populations from different regions.

Methods: Blood samples of 300 unrelated individuals from Chengdu (Han), Bangkok (Thai) and Maint (Germany) were taken and analyzed with single PCR, polyacrylamide gel electrophoresis and silver staining.

Results: In the three loci, 9, 6, and 8 alleles and 32,14, and 22 genotypes were found respectively.

[Analysis of the multi-amplified 5 STR loci and their allelic distribution in both Han populations in Chengdu City and Yunnan province].

Objective: To illuminate the multi-amplified 5 STR loci and their allelic distribution in Hans by means of STR-DNA typing with improved efficiency and decreased cost.

Methods: We have established an allelic ladder of D7S820, D13S317, D5S818, D3S1358 and Amelogenin loci via the cloning techniques. With this homemade allelic ladder, we established successfully a multiplexing polymerase chain reaction (PCR) method, followed by denaturing polyacrylamide gel electrophoresis (PAGE) and silver staining.

[Primary study on the influence of blood transfusion on the STR genotypes of recipient's blood].

Objective: To investigate whether any change occurred in recipients' blood collected at different times after transfusion with different quantity of blood.

Methods: Three patients were transfused with 400 ml, 800 ml and 1200 ml blood separately. The blood samples were collected from the recipients before transfusion and at 4 h, 8 h, 12 h after transfusion, and from the donors.

[A study of forensic individual identification used STR locus with silver staining and multiplex PCR methods].

Amplification of short tandem repeat(STR) loci has become a useful tool for human identification applications. To improve throughput and efficiency for the forensic materials and gain foure and six STR locis multiplex methods with silver staining, CSF1PO,TPOX,THO1 and vWA(referred to as multiplex A), D18S51, D7S820, D13S317, D5S818, D3S1358 and Amelogenin(referred to as multiplex B) have been evaluated for use in a rape case. The products of multiplex amplication were separated in a denaturing polyacrylamide gel and analyzed with silver staining.

[Genetic polymorphisms of fifteen short tandem repeat loci in Chengdu Han population].

Objective: To acquire the population genetic data of fifteen short tandem repeat (STR) loci in Chengdu Han population.

Methods: A total of 210 EDTA-blood specimens were collected from the unrelated individuals in Chengdu Han population. The DNA samples were extracted with Chelex method and amplified by multiplex PCR technique.

[Analysis of p53 gene mutation in esophageal exfoliative cells].

Objective: To explore the feasibility of detecting p53 gene mutation in exfoliative esophageal cells, and compare gene mutation between precancerous lesions and normal esophageal exfoliative cells and correlate p53 gene mutation with esophageal carcinogenesis.

Methods: Forty-eight samples (24 normal squamous epithelia and 24 severe squamous dysplasia) were obtained by balloon cytologic technique from a high incidence area, Yanting county, Sichuan Province, China in 1982. p53 gene mutations in exons 5 and 7 were analyzed by PCR-SSCP.