Current agricultural practices face numerous challenges, including declining soil fertility and heavy reliance on chemical inputs. Rhizosphere microbial metabolites have emerged as promising agents for enhancing crop health and yield in a sustainable manner. These metabolites, including phytohormones, antibiotics, and volatile organic compounds, play critical roles in promoting plant growth, boosting resistance to pathogens, and improving resilience to environmental stresses.
View Article and Find Full Text PDFThe anode-free lithium metal battery (AF-LMB) demonstrates the emerging battery chemistry, exhibiting higher energy density than the existing lithium-ion battery and conventional LMB empirically. A systematic step-by-step while bottom-up calculation system is first developed to quantitatively depict the energy gap from theory to practice. The attainable high energy of AF-LMB necessitates a homogeneous Li flux on the anode side to achieve an improved Li reversibility against inventory loss.
View Article and Find Full Text PDFIn , both males and females release chemical signals to attract mates. In our previous study, we identified ethyl laurate, ethyl myristate, and ethyl palmitate as potent female-derived pheromones that contribute to mate attraction. However, the mechanisms underlying the olfactory recognition remain unclear.
View Article and Find Full Text PDFBackground: Pyriproxyfen is an insect growth regulator (IGR) that is effective against various types of insect pests. However, the molecular mechanism underlying pyriproxyfen effects on insect reproduction remains unclear. Thus, in this study, we attempted to uncover the mechanisms underlying the impact of pyriproxyfen on the reproductive system of the model organism Drosophila melanogaster.
View Article and Find Full Text PDFTo break through the bottleneck in preparation of nanobody (Nb) for chemical contaminants induced by the difficulties in the synthesis of immunogen, complexity and unexpectable efficiency of immunization, a novel strategy to generate Nbs based on the designed synthetic Nb libraries with final size up to 10 cfu/mL was adopted and succeeded in selection of anti-coumaphos Nb A4. Furthermore, an affinity-matured mutant Nb 3G was obtained from the secondary library. Finally, an ic-ELISA was established with the limit of detection for coumaphos low to 1.
View Article and Find Full Text PDFEnantioselective antibodies have emerged as great potential biomaterials in the fields of immunoassays and chiral separation. However, cross-reactivity of antibodies to the distomer may severely restrict the application. Comprehending the interaction mechanism between antibodies and enantiomers could be beneficial to produce superior enantioselective antibodies.
View Article and Find Full Text PDFBackground: The Oka varicella vaccine strain remains neurovirulent and can establish lifelong latent infection, raising safety concerns about vaccine-related herpes zoster. In this study, we aimed to evaluate the immunogenicity and safety of a skin-attenuated and neuro-attenuated varicella vaccine candidate (v7D vaccine).
Methods: We did this randomised, double-blind, controlled, phase 2a clinical trial in Jiangsu, China.
Further increasing the critical temperature and/or decreasing the stabilized pressure are the general hopes for the hydride superconductors. Inspired by the low stabilized pressure associated with Ce 4f electrons in superconducting cerium superhydride and the high critical temperature in yttrium superhydride, we carry out seven independent runs to synthesize yttrium-cerium alloy hydrides. The synthetic process is examined by the Raman scattering and X-ray diffraction measurements.
View Article and Find Full Text PDFAs an emerging contaminant in soil, the impact of microplastics (MPs) on the environmental behavior of other organic pollutants remains uncertain, potentially threatening the sustainability of agricultural production. In this study, the impact of two kinds of MPs on the environmental behaviors of herbicide atrazine in soil-plant system was investigated. The results showed that MPs significantly reduced the half-life 17.
View Article and Find Full Text PDFProc Natl Acad Sci U S A
December 2023
Chemical communication plays a vital role in mate attraction and discrimination among many insect species. Here, we document a unique example of semiochemical parsimony, where four chemicals act as both aphrodisiacs and anti-aphrodisiacs in different contexts in . Specifically, we identified four female-specific semiochemicals, ethyl laurate, ethyl myristate, ethyl -9-hexadecenoate, and ethyl palmitate, which serve as aphrodisiacs to attract male flies and arouse male courtship.
View Article and Find Full Text PDFPestic Biochem Physiol
November 2023
The female reproductive potential plays a crucial role in reproduction, population dynamics and population maintenance. However, the function of endogenous genes in undifferentiated germ cells has been largely unknown in Bactrocera dorsalis. In this study, the conservative analysis showed that α-Spectrin shared a similarity in B.
View Article and Find Full Text PDFClathrate hydrideFm3-m-LaHhas been proven as the most extraordinary superconductor with the critical temperatureabove 250 K upon compression of hundreds of GPa in recent years. A general hope is to reduce the stabilization pressure and maintain the highvalue of the specific phase in LaH. However, strong structural instability distortsFm3-mstructure and leads to a rapid decrease ofat low pressures.
View Article and Find Full Text PDFThe interaction manner and biological function of Rab7 and its effector, Rab-interacting lysosomal protein (RILP), remain unclear in invertebrates. We provide a protocol for detecting the effects of Rab7 and RILP terminals on lysosome and autophagy in Spodoptera frugiperda Sf9 cells with overexpression and RNA interference. We describe steps for overexpressing plasmids, generating long double-stranded RNA, and transfecting them into Sf9 cells.
View Article and Find Full Text PDFTo explore carbide superconductors with higher transition temperature, two novel carbon structures of cage-network are designed and their superconductivity is studied by doping metals. MC and MC are respectively identified as C and C cage-network structures. This study finds that both carbon structures drive strong electron-phonon interaction and can exhibit superconductivity above liquid nitrogen temperature.
View Article and Find Full Text PDFBackground: The real-world data of long-term protection under moderate vaccination coverage is limited. This study aimed to evaluate varicella epidemiology and the long-term effectiveness under moderate coverage levels in Ganyu District, Lianyungang City, Jiangsu Province.
Methods: This was a population-based, retrospective birth cohort study based on the immunization information system (IIS) and the National Notifiable Disease Surveillance System (NNDSS) in Ganyu District.
The heightened concern about the environmental impacts of pollutants drives interest in reducing their threats to humans and the environment. Bioremediating polluted sites under environmental stresses like biotic and abiotic poses significant challenges. This study aimed to isolate a bacterium that effectively degrades pyrethroids even under abiotic stresses involving heavy metals and biotic stresses with autochthonous factors.
View Article and Find Full Text PDFMicroorganisms can drive a substrate-specific biodegradation process to mitigate soil contamination resulting from extensive agrochemical usage. However, microorganisms with high metabolic efficiency are capable of adapting to the co-occurrence of non-substrate contaminants in the soil (particularly antibiotics). Therefore, the utilization of active microorganisms for biodegradation raises concerns regarding the potential risk of antibiotic resistance development.
View Article and Find Full Text PDFDrosophila ovarian germline stem cells (GSCs) are a powerful model for stem cell research. In this study, we use single-cell RNA sequencing (scRNA-seq), an RNAi screen and bioinformatic analysis, to identify genes involved in germ cell differentiation, including 34 genes with upregulated expression during early germ cell development and 19 genes that may regulate germ cell differentiation. Among these, a gene we have named eggplant (eggpl) is highly expressed in GSCs and downregulated in early daughter cells.
View Article and Find Full Text PDFConducting atomic force microscopy has been performed for a fundamental understanding of the mechanism responsible for the lower power conversion efficiency (PCE) of CuZnSnS (CZTS) solar cells than that of CuInGaSe (CIGS) solar cells. The difference in efficiency is partly attributed to the distinctly different band alignment between the grain boundaries (GBs) and grain interior (GI) for the two materials. While CIGS shows type-II band alignment, CZTS was discovered to demonstrate type-I band alignment with the conduction band shifting downward while the valence band shifting upward at the GBs.
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