[Comparative clinical observation of the short-term bone fusion effect of composite biosynthetic bone and ilium auto-grafting].

Objective: To evaluate the short-term bone fusion after implantation of composite biosynthetic bone made of coralline hydroxyapatite composite (CHC) as a substitute for autologous bone grafting.

Methods: A clinical sequential trial was designed and the biosynthetic bone and the patient's ilium were respectively used in the treatment of femoral nonunions and interbody fusion. X-ray examinations were performed 10 weeks after the operations and the bone fusion was graded according to Lane-Sandhu's method.

Cloning of GAP Gene from K. fragilis.

The K. fragilis CBS 397 gene library was screened with a GAP probe, which was designed according to the homology with S. cerevisiae, K.

Cloning of DNA Fragments Possessing Promoter Function from Kluyveromyces cicerisporus.

Using three yeast promoter-probe plasmids pSK-kan401, pSK-kan1105, pSK-kan1238 with different reading frames, eight DNA fragments possessing high efficient promoter function have been cloned from K. cicerisporus. Their 3'-DNA sequences have been analyzed.