RNA m6A reader YTHDF1 facilitates inflammation via enhancing NLRP3 translation.

N-methyladenosine (m6A) modification of mRNAs is involved in multiple essential biological processes, dynamically regulated by m6A "writers", "erasers", and "readers". Yet, the detailed functional roles of RNA m6A reader proteins, such as YTHDFs, are largely unknown. Herein we show that YTHDF1 promotes pro-inflammatory IL-1β production in macrophages during bacterial infections.

CyclinD1 Is a New Target Gene of Tumor Suppressor MiR-520e in Breast Cancer.

Objective: To investigate the involvement of miR-520e in the modulation of cancer-promoting cyclinD1 in breast cancer.

Methods: A reverse transcription-polymerase chain reaction (RT-PCR) was applied to test the regulation of miR-520e on cyclinD1. The binding of miR-520e to 3'-untranslated region (3'UTR) of cyclinD1 mRNA was predicted by an online bioinformatics website.

[Simple self-correlative algebraic reconstruction technique].

A new improvement was brought forward in algebraic reconstruction technique (ART), named simple self-correlative algebraic reconstruction technique (SSCART), and its reconstructive result was discussed in detail. With numerical simulation technique, both ART and SSCART were numerically simulated with computer, and discussed in terms of simulated result and reconstructive accuracy with error indexes such as mean-square error (MSE), absolute value error (AVE) and peak error (PE). As a result, the accuracy of SSCART was markedly improved.

[Analyzing the methods to smooth field reconstructed by algebraic reconstruction technique with spectroscopy].

The effects of filters and filtering methods on the field reconstructed by algebraic reconstruction technique (ART) is discussed with spectroscopy. The field to be constructed was simulated with numerical simulation technique. The effects of many kinds of filters and filtering methods on the field reconstructed by ART were analyzed with spectroscopy.