Epigenetic regulation of VENTXP1 suppresses tumor proliferation via miR-205-5p/ANKRD2/NF-kB signaling in head and neck squamous cell carcinoma.

An increasing number of studies have shown that long noncoding RNAs (lncRNAs) play important roles in tumor development and progression. However, their involvement in head and neck squamous cell carcinoma (HNSCC) remains largely unknown. Epigenetic regulation is one major mechanism utilized by cancer cells to control lncRNA expression.

Long non-coding RNA ANRIL promotes tumorgenesis through regulation of FGFR1 expression by sponging miR-125a-3p in head and neck squamous cell carcinoma.

ANRIL (CDKN2B antisense RNA 1, CDKN2B-AS1) is involved in the progression of various cancers. However, its role in head and neck squamous cell carcinoma (HNSCC) remains unclear. In this study, we found that ANRIL expression was upregulated in HNSCC and correlated with tumor progression.

STT3-dependent PD-L1 accumulation on cancer stem cells promotes immune evasion.

Enriched PD-L1 expression in cancer stem-like cells (CSCs) contributes to CSC immune evasion. However, the mechanisms underlying PD-L1 enrichment in CSCs remain unclear. Here, we demonstrate that epithelial-mesenchymal transition (EMT) enriches PD-L1 in CSCs by the EMT/β-catenin/STT3/PD-L1 signaling axis, in which EMT transcriptionally induces N-glycosyltransferase STT3 through β-catenin, and subsequent STT3-dependent PD-L1 N-glycosylation stabilizes and upregulates PD-L1.

[Safety and efficacy of ultrasound hyperthermia combined with chemotherapy and radical operation for advanced oral squamous cell carcinoma].

Purpose: To explore the safety and efficacy of ultrasound hyperthermia combined with chemotherapy and radical operation for advanced oral squamous cell carcinoma (OSCC).

Methods: Nine patients who had definite pathological diagnosis were enrolled in this clinical trail from 2015 to 2016. All patients underwent ultrasound hyperthermia combined with chemotherapy before radical operation.

Long-term results of a randomized phase III trial of TPF induction chemotherapy followed by surgery and radiation in locally advanced oral squamous cell carcinoma.

Previously, we conducted a randomized phase III trial of TPF (docetaxel, cisplatin, and 5-fluorouracil) induction chemotherapy in surgically managed locally advanced oral squamous cell carcinoma (OSCC) and found no improvement in overall survival. This study reports long-term follow-up results from our initial trial. All patients had clinical stage III or IVA locally advanced OSCC.

Randomized phase III trial of induction chemotherapy with docetaxel, cisplatin, and fluorouracil followed by surgery versus up-front surgery in locally advanced resectable oral squamous cell carcinoma.

Purpose: To evaluate induction chemotherapy with docetaxel, cisplatin, and fluorouracil (TPF) followed by surgery and postoperative radiotherapy versus up-front surgery and postoperative radiotherapy in patients with locally advanced resectable oral squamous cell carcinoma (OSCC).

Patients And Methods: A prospective open-label phase III trial was conducted. Eligibility criteria included untreated stage III or IVA locally advanced resectable OSCC.

Expression of podoplanin in salivary gland adenoid cystic carcinoma and its association with distant metastasis and clinical outcomes.

Distant metastasis is a common cause of mortality in patients with salivary gland adenoid cystic carcinoma (SACC). However, presently, the development of distant metastasis is unable to be predicted in clinical practice. Recent studies have shown that overexpression of podoplanin is associated with metastasis and survival in patients with several cancer types.

Contactin 1 (CNTN1) expression associates with regional lymph node metastasis and is a novel predictor of prognosis in patients with oral squamous cell carcinoma.

The contactin 1 (CNTN1) gene exerts oncogene‑like activities and its expression has been linked to several human malignancies. In this study, a possible association between CNTN1 expression and clinicopathological parameters and clinical outcomes in patients with oral squamous cell carcinoma (OSCC) was examined. CNTN1 protein expression was evaluated by immunohistochemistry in OSCC tissues of 45 patients.

Primary oral mucosal melanoma: advocate a wait-and-see policy in the clinically N0 patient.

Purpose: Oral mucosal melanoma (OMM) is a rare disease associated with a very poor prognosis. Because well-established treatment protocols for OMM are in short supply, prognostic information regarding recent treatment modalities for this disease were sought.

Patients And Methods: A retrospective chart review was performed of 61 patients who were treated for OMM from 1998 through 2005.

Establishment and characterization of a rabbit oral squamous cell carcinoma cell line as a model for in vivo studies.

The incidence of oral squamous cell carcinoma (SCC) is increasing but the long-term survival rate remains low. An animal model would therefore be helpful for evaluation of new treatment modalities for oral SCC. Hamster is small animal, therefore, the cancer of hamster cheek pouch is not optimal for tumor imaging.

Neck dissection and post-operative chemotherapy with dimethyl triazeno imidazole carboxamide and cisplatin protocol are useful for oral mucosal melanoma.

Background: Oral mucosal melanoma (OMM) is a clinically rare disease with poor prognosis. Various treatment methods have been investigated with the aim of improving the prognosis. This study aimed to analyze the data of a single institution in the management of OMM.

[Inhibition effects of tumor necrosis factor-alpha gene transduced tumor drainage node of lymphocytes from tongue cancer on SCID mice transplanted tumor established with human tongue carcinoma cell lines].

Objective: To observe the in vivo inhibition effects of tumor necrosis factor-alpha (TNF-alpha) gene transduced tumor drainage node of lymphocytes (DNL) from tongue cancer on SCID mice transplanted tumor.

Methods: 15 human tongue carcinoma models were established in SCID mice by subcutaneously injection of squamous cell carcinoma line Tca8113. TNF-alpha gene introduced DNL, combined with low dose Pinyancin (PYC), were locally injected into tumor site.

[Evaluation of fluorodeoxyglucose-position-emission tomography-computer tomography imaging on head and neck squamous cell carcinomas including primary tumors and lymph node metastases].

Objective: To evaluate (18)F-fluorodeoxyglucose-position-emission tomography-computer tomography imaging ((18)F-FDG-PET-CT) on head and neck squamous cell carcinoma(HNSCCA) and lymph node metastasis.

Methods: (18)F-FDG-PET-CT imaging of 20 patients with HNSCCA was evaluated retrospectively.

Results: All the primary tumors were correctly diagnosed by (18)F-PET-CT imaging and SUV(avg) of the primary tumors was (6.

Expression and importance of inhibitor of DNA binding helix-loop-helix protein in salivary adenoid cystic carcinoma.

Studies have reported that Id-1 (inhibitor of DNA binding, or differentiation 1) protein, helix-loop-helix (HLH) transcription factor, has important roles in the development of several carcinomas. However, the role of Id-1 protein in salivary adenoid cystic carcinoma (ACC) is not clear. The expression of Id-1 protein was assessed in formalin-fixed, paraffin-embedded surgical specimens of ACCs by immunohistochemical staining.

Expression of Glut-1 in primary and recurrent head and neck squamous cell carcinomas, and compared with 2-[18F]fluoro-2-deoxy-D-glucose accumulation in positron emission tomography.

The aim of the study was to investigate the pattern of glucose transporter-1 (Glut-1) expression in primary and recurrent head and neck squamous cell carcinomas (HNSCCAs) and the relation between Glut-1 expression and 2-[18F]fluoro-2-deoxy-D-glucose - positron emission tomography (FDG-PET). Standardised uptake values (SUVs) were used to evaluate FDG uptake by the tumour. Sections were stained immunohistochemically for Glut-1, which showed that high SUVs were seen in all HNSCCAs, and patients with higher T stage tumours or less well-differentiated tumours showed significantly higher SUVs than those with lower stage tumours or better-differentiated tumours (P=0.

[A study on the mechanism of inducing apoptosis of Tca8113 cells by means of ultrasound hyperthermia].

Purpose: To investigate the mechanism of apoptosis in Tca8113 cells induced by ultrasound hyperthermia by detecting changes in related index.

Methods: Tca8113 cells were treated in vitro by ultrasound hyperthermia in different heating temperatures (38 degrees C to 44 degrees C,10 minutes) and heating times (42 degrees C,10 to 60 minutes), and then the dynamic changes of early apoptosis and secondary necrosis treated by 42 degrees C for 10 minutes were assayed to detect deltapsim and Caspase-3 levels of different groups by flow cytometry (FCM). The means of each group were compared by ANOVA with SAS6.

[Genetic linkage analysis and mutation detection in Chinese families with basal cell nevus syndrome].

Objective: To study the molecular genetic etiology of a Chinese pedigree with basal cell nevus syndrome.

Methods: The proband and his affected mother and a unaffected individual in the pedigree were chosen and peripheral blood was collected from them for DNA. Direct sequencing was performed to detect the mutations of PTCH gene.

[Experimental study on ultrasound heating affecting the sensitivity of oral carcinoma to chemotherapy drugs].

Objective: To explore the influence of chemosensitivity of Tca8113 cells by modified MTT assay after the animal model of Tca8113 were treated by the ultrasound hyperthermia.

Methods: The MTT assay of the BALB/C nu/nu mice model of Tca8113 cells treated by the ultrasound hyperthermia in vivo was performed.

Results: The chemosensitivity to the 9 kinds of drugs demonstrated no significant differences between the Tca8113 cells in the control group, the 39 degrees C-treated group and the groups treated from 41 degrees C to 44 degrees C.