Publications by authors named "Guo-xiang Shi"
[Clone expression of the caf1 gene of Yersinia pestis and immunological evaluation on recombinant F1 antigen].
Zhonghua Liu Xing Bing Xue Za Zhi
January 2010
Objective: To get recombinant F1 antigen (rF1) and to construct the detection dipstick of plague antibody.
Methods: The caf1 gene removing the signal peptide coding sequence was cloned into plasmid pET32a(+) by double-digested sites of BamHI and Not I. Recombinant plasmid caf1-pET32a(+) was transformed into BL21 (DE3) and the rF1 was expressed.
[Study on the application and evaluation of methods for gene and antigen detection in plague surveillance program].
Zhonghua Liu Xing Bing Xue Za Zhi
May 2007
Article Synopsis
- The study aimed to improve gene and antigen detection methods within a plague surveillance program by testing 1798 samples.
- Researchers used various testing methods, including multiple-polymerase chain reaction and enzyme linked immunosorbent assays, alongside traditional culture techniques to identify Yersinia pestis.
- Results showed a positive detection rate of 7.34% with the new methods, which is a significant increase from 6.28%, indicating that the updated approaches may enhance plague diagnosis.