[Toxoplasma gondii Rhoptry Protein 17 Inhibits the Apoptosis of Mouse Macrophages via Activation of Activator Protein 1 Signaling].

Objective: To investigate the effect of Toxoplasma gondii rhoptry protein 17（ROP17） on γ-interferon (IFN-γ)-induced apoptosis of mouse J774A.1 monocyte macrophages.

Methods: The J774A.

[Research Advances on Gliding-associated Proteins of Toxoplasma gondii].

The ability to invade host cells is a key to the survival and pathogenicity of Apicomplexan parasites. Toxoplasma gondii is an obligatory intracellular parasite. Its motility, invasion into, and egression from host cells are powered by a machinery called acto-myosin motor （AMM）.

Recombinant Toxoplasma gondii phosphoglycerate mutase 2 confers protective immunity against toxoplasmosis in BALB/c mice.

Toxoplasmosis is one of the most widespread zoonoses worldwide. It has a high incidence and can result in severe disease in humans and livestock. Effective vaccines are needed to limit and prevent infection with Toxoplasma gondii.

DNA vaccination with a gene encoding Toxoplasma gondii Rhoptry Protein 17 induces partial protective immunity against lethal challenge in mice.

Toxoplasma gondii is an obligate intracellular apicomplexan parasite that affects humans and various vertebrate livestock and causes serious economic losses. To develop an effective vaccine against T. gondii infection, we constructed a DNA vaccine encoding the T.

[Research Advance on Toxoplasma gondii Rhoptry Protein 2 Family as Candidate Vaccine Molecules].

Toxoplasma gondii rhoptry protein 2 family (ROP2 family), secreted by the rhoptry, plays an important role in T. gondii invasion of host cells and its virulence. The ROP2 family members include ROP2, ROP4, ROP5, ROP8, ROP13, ROP16, ROP17, and ROP18.

[Research progress on uridine phosphorylase in vertebrate and parasites].

Uridine phosphorylase (UPP) is a key enzyme of pyrimidine salvage pathways, catalyzing the reversible phosphorolysis of ribosides of uracil to nucleobases and ribose 1-phosphate. UPP plays an important role in the regulation of uridine homeostasis. Although UPP from a variety of organisms have many similarities in their functions, there are differences in many other aspects, such as physical and chemical properties, structure characteristics, active sites, and substrate binding sites.

Protective immunity induced by peptides of AMA1, RON2 and RON4 containing T-and B-cell epitopes via an intranasal route against toxoplasmosis in mice.

Background: Toxoplasma gondii is a ubiquitous protozoan intracellular parasite, the causative agent of toxoplasmosis, and a worldwide zoonosis. Apical membrane antigen-1 (AMA1) and rhoptry neck protein (RON2, RON4) are involved in the invasion of T. gondii.

Partial protective effect of intranasal immunization with recombinant Toxoplasma gondii rhoptry protein 17 against toxoplasmosis in mice.

Toxoplasma gondii (T. gondii) is an obligate intracellular protozoan parasite that infects a variety of mammals, including humans. An effective vaccine for this parasite is therefore needed.

[Construction, expression and kinase function analysis of an eukaryocyte vector of rhoptry protein 17 in Toxoplasma gondii].

Objective: To construct and express the eukaryocytic expression vector of rhoptry protein 17 of Toxoplasma gondii RH strain (TgROP17) and analyze its kinase function.

Methods: The open reading frame of TgROP17 gene was amplified from total RNA in T. gondii RH strain by RT-PCR, and cloned into p3 x Flag-CMV-14 vector to construct recombinant plasmid p3xFlag-CMV-14/TgROP17.

[Gene-cloning, expression and immunoreactivity detection of Toxoplasma gondii uridine phosphorylase].

Objective: To predict the physicochemical properties and antigenic epitopes of Toxoplasma gondii uridine phosphorylase (TgUPase), clone, and express TgUPase gene, and analyze its immunoreactivity.

Methods: The physical and chemical characters and specific epitopes of TgUPase protein were predicted by bioinformatics software tools. Total RNA was extracted from RH strain T.

[Cloning and expression of actin gene of Toxoplasma gondii].

Objective: To clone and express the actin gene of Toxoplasma gondii, and analyze the immunoreactivity of the recombinant protein.

Methods: Total RNA was extracted from tachyzoites of RH strain of T. gondii.

Intranasal immunisation of the recombinant Toxoplasma gondii receptor for activated C kinase 1 partly protects mice against T. gondii infection.

Nasal vaccination is an effective therapeutic regimen for preventing certain infectious diseases. The mucosal immune response is important for resistance to Toxoplasma gondii infection. In this study, we evaluated the immune responses elicited in BALB/c mice by nasal immunisation with recombinant T.

Intranasal immunisation with recombinant Toxoplasma gondii actin partly protects mice against toxoplasmosis.

Toxoplasma gondii is a ubiquitous protozoan intracellular parasite, the causative agent of toxoplasmosis, and a worldwide zoonosis for which an effective vaccine is needed. Actin is a highly conserved microfilament protein that plays an important role in the invasion of host cells by T. gondii.

Toxoplasma gondii protein disulfide isomerase (TgPDI) is a novel vaccine candidate against toxoplasmosis.

Toxoplasma gondii is a ubiquitous protozoan parasite that can infect all warm-blooded animals, including both mammals and birds. Protein disulfide isomerase (PDI) localises to the surface of T. gondii tachyzoites and modulates the interactions between parasite and host cells.

[Cloning, expression and immunogenicity analysis of malate dehydrogenase gene of Toxoplasma gondii].

Objective: To clone and express the malate dehydrogenase (MDH) gene of Toxoplasma gondii, and analyze the immunogenicity.

Methods: Total RNA was extracted from tachyzoites of RH strain of T. gondii (GenBank accession No.

[Efficacy of albendazole orally administered at different dosages against Trichinella spiralis encapsulated larvae in mice].

Objective: To evaluate the efficacy of albendazole (ABZ) orally administered at different dosages against Trichinella spiralis encapsulated larvae in striated muscle in mice.

Methods: A total of 72 BALB/c mice were divided equally into 9 groups. Each mouse was infected orally with 50 T.

[Research progress in phosphoglycerate mutase].

Phosphoglycerate mutase (PGAM) is one of glycolytic enzymes, concerning with the transport of carbohydrates, metabolism, catalytic activity and growth development. PGAM was discovered in yeast firstly, and with its amino acid sequence and crystal structure determined, this protein was found in varies organism, such as human, Escherichia coli, Schistosoma japonicum and Toxoplasma gondii. This article reviews the physico-chemical property and research progress of PGAM of vertebrate, invertebrate and protozoa.

Gene-cloning, expression and antigenicity analysis of rhoptry protein 17 of Toxoplasma gondii.

Objective: To clone and express the rhoptry protein 17 (ROP17) gene of RH strain of Toxoplasma gondii, analyze the antigenicity of recombinant protein.

Methods: Total RNA was extracted from tachyzoites of RH strain of T. gondii.

[Cloning, expression and antigenicity analysis of phosphoglycerate mutase 2 gene of Toxoplasma gondii].

Objective: To clone and express the phosphoglycerate mutase 2 (PGAM2) gene of Toxoplasma gondii, and analyze the antigenicity of the recombinant protein.

Methods: Total RNA was extracted from T. gondii tachyzoites of RH strain and reversely transcribed into cDNA.

[Effect of oral administration of tribendimidine at different dosages against Trichinella spiralis encapsulated larvae in mice].

Objective: To observe the efficacy of oral administration of tribendimidine (TBD) at different dosages against Trichinella spiralis encapsulated larvae in murine striated muscle.

Methods: A total of 88 BALB/c mice were divided equally into 11 groups. Each mouse was infected orally with 50 T spiralis encapsulated larvae.

[Kinetics of IgA secreting cells and sIgA in small intestine of mice induced by intranasal immunization with Toxoplasma gondii STAg].

Objective: To investigate the kinetics of IgA secreting cells (IgASCs) and secretory IgA (sIgA) level in small intestine induced by intranasal immunization with Toxoplasma gondii soluble tachyzoite antigen (STAg) in mice.

Methods: Ninety-six 5 to 6-week old BALB/c mice were randomly divided into immunity and control groups. Mice of the immunity group were each intranasally immunized with STAg 20 microg in 20 microl PBS, twice at an interval of 2 weeks, while the control mice were each given 20 microl PBS.

[Early kinetics of Toxoplasma gondii infection in mice infected intragastrically with tachyzoites by chromogenic in situ hybridization targeting SAG2 mRNA].

Objective: To observe the early kinetics of Toxoplasma gondii infection in mice inoculated with tachyzoites of RH strain.

Methods: Twenty BALB/c mice were administered intragastrically with tachyzoites of RH strain (2 x 10(4)/mice). Parasite burdens in mesenteric lymph node (MLN), liver, spleen, lung and brain were determined by chromogenic in situ hybridization targeting SAG2 mRNA at 1, 2, 4, 6 and 8 days postinfection.

[Kinetics of IgA secreting cells and IgA in small intestine of mice induced by Toxoplasma gondii tachyzoite infection].

Objective: To investigate the kinetics of IgA secreting cells (IgASCs) in small intestine and the specific antibody level induced by Toxoplasma gondii tachyzoite infection in mice.

Methods: Ninety-six BALB/c mice were randomly divided into 2 groups, 12 were intragastrically given 0.5 ml PBS as control, the rest were each intragastrically infected with 1 x 10(4) tachyzoites of the virulent RH strain Toxoplasma gondii.

Toxoplasma gondii: proteomic analysis of antigenicity of soluble tachyzoite antigen.

The obligate intracellular parasite Toxoplasma gondii is an important pathogen of humans and animals. The tachyzoite of T. gondii is the main life-cycle stage that is responsible for toxoplasmosis.

[Kinetic observation on the invasion into and proliferation in IEC-6 cells of Toxoplasma gondii RH strain tachyzoites in vitro].

Objective: To study the invasion and proliferation in IEC-6 cells of Toxoplasma gondii RH strain tachyzoites in vitro.

Methods: T. gondii tachyzoites of RH strain were co-cultured with IEC-6 cells in vitro, the process of cell adhesion, invasion and proliferation by tachyzoites was observed consecutively with inverted microscope.