Selenious-β-lactoglobulin induces the apoptosis of human lung cancer A549 cells via an intrinsic mitochondrial pathway.

In this study, the cytotoxic activity of selenious-β-lactoglobulin (Se-β-Lg) and the anticancer mechanism were investigated in human lung cancer A549 cells in vitro. MTT assay showed that Se-β-Lg at 200 μg/mL exhibited a significant suppression effect on A549 cells and the maximum inhibition rate reached 90% after 72 h treatment. Flow cytometry analysis revealed that 200 μg/mL of Se-β-Lg induced cell cycle arrest at G/G phase.

Selenium modification of β-lactoglobulin (β-Lg) and its biological activity.

β-Lg is a major whey protein in cow's milk. This study was aimed to find a new kind of organic selenium compound synthesized with β-Lg and selenium dioxide as raw materials under the conditions of vacuum and low temperature. Fourier transformed infrared spectroscopy revealed that seleno-β-lactoglobulin (Se-β-Lg) displayed a strong band at 878cm(-1), belonging to SeO.

Effect of spleen lymphocytes on the splenomegaly in hepatocellular carcinoma-bearing mice.

Objective: To study the effect of spleen lymphocytes on the splenomegaly by hepatocellular carcinoma-bearing mouse model.

Methods: Cell counts, cell cycle distribution, the percentage of lymphocytes subsets and the levels of IL-2 were measured, and two-dimensional gel electrophoresis (2-DE) was used to investigate the relationship between spleen lymphocytes and splenomegaly in hepatocellular carcinoma-bearing mice.

Results: Compared with the normal group, the thymus was obviously atrophied and the spleen was significantly enlarged in the tumor-bearing group.

Immunizing effects of cocultures of H22 hepatocarcinoma cells and cartilage polysaccharide on murine H22 hepatocarcinoma.

Hepatocarcinoma is a malignant cancer, which is threatening human lives. In order to disclose the immunizing effects of the cells and cartilage polysaccharide (CHCP) on liver cancer, murine H22 hepatocarcinoma model was set up. The survival time, life span, and survival rate of the CHCP group were better than model group or other groups which was immunized with cartilage short-chain polysaccharide (CPS) only or H22 cell lysate only.

[Identification of novel genes related to glioma by oligonucleotide microarray].

Objective: To identify the genes differentially expressed in development of human glioma, and to study the expression of some genes in different grade gliomas.

Methods: Oligonucleotide microarray (including 218 genes related to neural system development) was adopted and hybridized with probes which were prepared from the total RNAs of glioma specimens and normal brain tissues. Differentially expressed genes between the normal tissues and glioma tissues were assayed after scanning oligonuceltide microarray with ScanArray 4000, and some of these genes such as smad1, Hmp19 and TRIP3 were verified by real-time quantitative PCR(real-time-Q-PCR) method.