[Establishment and application of TaqMan Real-Time PCR in detection and serogrouping of Neisseria meningitidis].

Objective: To establish TaqMan Real-Time PCR method for detection and identification of Neisseria meningitidis.

Methods: Seven sets of primers and FAM-labeled probes targeting different genes of Neisseria meningitidis were designed and synthesized. ctrA gene was used for identification of N.

[Establishment of fluorescent amplified fragment length polymorphism in Vibrio cholerae and evaluation in molecular typing].

Objective: To develop fluorescent amplified fragment length polymorphism (AFLP) method and to evaluate the its typing capability with pulsed-field gel electrophoresis (PFGE) in molecular typing of Vibrio cholerae.

Methods: Forty-seven strains of V. cholerae, with different PFGE patterns, were selected as the reference group to optimize the selective primers of AFLP analysis.

[Sequence analysis of housekeeping genes recA, dnaE, and mdh in different serogroups or biotypes of Vibrio cholerae isolated from China].

Objective: To analyze sequences of the housekeeping genes including recA, dnaE, and mdh in different serogroups or different biotypes of Vibrio cholerae (VC) strains isolated from China.

Methods And Results: recA, dnaE, and mdh genes of Vibrio cholerae were obtained by PCR, sequenced and analyzed. Forty-four variable bases were identified in the 500 bases of recA gene of 18 VC strains, and the mutation of only 3 variable bases could result in changes of 2 amino acids.

[Functional analysis of promoters of Vibrio cholerea typing phage VP1 with reporter system].

Phage VP1 infects and lyses Vibrio cholerae. The VP1 genome is a circular double-strand DNA and its size is 32176 base pairs. Analysis of the sequence of the VP1 genome revealed the presence of 15 putative promoter sequence.

[Study on infection of different strains of Vibro cholerae O1 by El Tor CTXPhi].

To study the horizontal transfer efficiencies of filamentous bacteriophage CTXPhi in different V. cholera O1 strains and the phage immunities of these strains. The infectious El Tor CTXPhi particles genetic marked by chloramphenicol resistance gene were used to infect four different V.

[Sequence analysis of the mutated genes in CTXEVC Phi and nct-CTXnew Phi genomes in Vibrio cholerae JS94484 strain].

Objective: To analyze the sequences of the mutated genes in CTX(EVC)Phi and nct-CTX(new)Phi genomes in Vibrio cholerae JS94484 strain.

Methods: The mutated genes in CTX(EVC)Phi and nct-CTX(new)Phi genome were obtained by PCR, sequenced and analyzed.

Results: ig1, rstR, ig2, and ctxAB genes in CTX(EVC)Phi genome of V.

[Analysis of gene cluster of Tat-dependent protein export system of Vibrio cholerae and its function].

The Tat (Twin-arginine translocatin) system is a recently defined protein export pathway that serves to translocate folded proteins. The substrates of the Tat pathway contain specific amino-terminal signal peptides that exhibit a conserved amino acid consensus motif-S/T-R-R-x-F-L-X. Here is the report of knocked out the tatA, tatB and tatC genes of the V.