[Effects of icariin on the proliferation, differentiation and maturation of rat calvarial osteoblasts in vitro].

Objective: To investigate the effects of icariin on the proliferation, differentiation and maturation of rat calvarial osteoblasts (ROB).

Methods: Segregated neonatal SD rat skull,enzyme digestion to obtain ROB. The culture medium was replaced every three days.

[The changes of iNOS and NO in the osteogenic differentiation process of rat bone marrow stromal cells promoted by icariside II].

This study is to investigate the effects on the expression of iNOS and production of NO in the osteogenic differentiation process of rat bone marrow stromal cells (rBMSCs) by icariside II. rBMSCs were cultured by adherence screening method. When the culture dishes were covered with 80% cells, the osteogenic induced cultures were adopted.

Osthol, a coumarin isolated from common cnidium fruit, enhances the differentiation and maturation of osteoblasts in vitro.

The effect of osthol on osteoblasts was investigated in primary osteoblastic cells isolated from newborn Wistar rats. Osthol was supplemented into cultured medium at 10⁻⁷, 10⁻⁶, 10⁻⁵ and 10⁻⁴ mol/l, respectively. No stimulating effect was found on cell proliferation, but 10⁻⁵ mol/l osthol caused a significant increase in alkaline phosphatase (ALP) activity.