Enhancing disease resistances of Super Hybrid Rice with four antifungal genes.

A plant expression vector harboring four antifungal genes was delivered into the embryogenic calli of '9311', an indica restorer line of Super Hybrid Rice, via modified biolistic particle bombardment. Southern blot analysis indicated that in the regenerated hygromycin-resistant plants, all the four antifungal genes, including RCH10, RAC22, beta-Glu and B-RIP, were integrated into the genome of '9311', co-transmitted altogether with the marker gene hpt in a Mendelian pattern. Some transgenic R1 and R2 progenies, with all transgenes displaying a normal expression level in the Northern blot analysis, showed high resistance to Magnaporthe grisea when tested in the typical blast nurseries located in Yanxi and Sanya respectively.

[Genetic analysis and SSR marker of tolerance to submergence in rice].

Submergence stress has severe harm to rice (Oryza sativa L.) production. It is important to identify molecular markers associated with tolerance to submergence for marker-assisted selection (MAS).