Role of hunchback in segment patterning of Locusta migratoria manilensis revealed by parental RNAi.

In long germ embryos, all body segments are specified simultaneously during the blastoderm stage. In contrast, in short germ embryos, only the anterior segments are specified during the blastoderm stage, leaving the rest of the body plan to be specified later. The striking embryological differences between short and long germ segmentation imply fundamental differences in patterning at the molecular level.

[Cloning and characterization of the neutral trehalase gene in Metarhizium anisopliae CQMa102].

The partial fragment of the neutral trehalase (NTL) in Metarhizium anisopliae CQMa102 was amplified by the polymerase chain reaction (PCR) with primers designed according to the sequences of the NTL in GenBank. The amplified fragment was cloned and sequenced. Based on the known sequence of NTL gene, the 5' and 3' rapid amplification of cDNA ends (RACE) were used to amplify the 5' and 3'regions of the NTL cDNA, then the whole cDNA sequence of NTL gene in M.