Blood cadmium levels and overactive bladder in middle-aged and older adults in the United States: Insights from NHANES 2007-2020 data.

Cadmium is a common environmental pollutant associated with various health risks. Its neurotoxic, muscle-damaging, and pro-inflammatory properties may be related to overactive bladder (OAB), though few studies have assessed its impact on urinary function. This study aimed to examine the potential link between cadmium exposure and OAB.

[Research progress on mechanism and preventive measures of retrograde ejaculation after benign prostatic hyperplasia surgery].

Benign prostatic hyperplasia (BPH) is a common disease in middle-aged and elderly men. It's first-line therapy is drugs. But with the progression of the disease or side effects of drugs, surgical treatment will become a better choice.

[Effects of human cytomegalovirus on the cell cycle of duct epithelial cell cultures of human salivary gland in vitro and relative mechanism].

Objective: To investigate the effects of human cytomegalovirus (HCMV) on the cell cycle of duct epithelial cell cultures of human salivary gland (HSG) in vitro and relative mechanism.

Methods: HSG was cultured in vitro. Reverse transcriptase polymerase chain reaction (RT-PCR) and nest-RT-PCR were used respectively to investigate ie1/ie2 transcription in HSG infected by human cytomegalovirus(HCMV).

[Expression of Toll-like receptors in human bone marrow mesenchymal stem cells].

The aim of this study was to explore the characteristics of Toll-like receptor expression in mesenchymal stem cells derived from bone marrow of healthy donor (BM-MSCs). BM-MSCs were isolated from bone marrow of healthy donor by Ficoll method. Expressions of CD34, CD45, HLA-DR, CD44 and CD71 in BM-MSCs were detected by flow cytometry.

[Effect of landscape pattern on mouse pest in Dongting Lake area].

The relationship between landscape pattern and ecological process is one of the key issues in landscape ecology. Both the species protection and the bio-disaster prevention are closed linked with the landscape pattern of organism's habitat. Taking Dongting Lake area as a case, this paper studied the characteristics of landscape pattern in typical sampling plots, and analyzed the correlation of mouse capture rate in farmland and in house with the indices reflecting the characteristics of landscape pattern.

Glucosamine sulfate-induced apoptosis in chronic myelogenous leukemia K562 cells is associated with translocation of cathepsin D and downregulation of Bcl-xL.

Cathepsin D (cat D) reportedly plays an important role in certain apoptotic processes, the downstream pathways of which involve release of cytochrome c (cyt c) from mitochondria and activation of the caspase cascade. Previous studies revealed that the B-cell lymphoma 2 (Bcl-2) family members Bax or Bid play important roles in apoptotic signal transduction between cat D and mitochondria. Here, we show that glucosamine sulfate (GS) inhibits the proliferation and induces apoptosis of human chronic myelogenous leukemia K562 cells in vitro.

[Genetic diversity and population structure of BMY and brahman cattle revealed by six microsatellite loci].

The genetic variations and polymorphisms of six microsatellite loci were analyzed to determine the population structure and breeding progress of BMY and Brahman cattle. The range of polymorphic information content of six loci was 0.524-0.

[Effects of HCMV on phenotypes of parotid duct epithelial cells and its mechanisms].

Objective: To investigate the effects of HCMV infection on phenotypes of parotid duct epithelial cells and relative mechanisms.

Methods: The expressions of immediate early antigen of HCMV, pan cytokeratin and cathepsin D etc. were detected by immunohistochemical staining in tissues of parotid cytomegalic inclusion disease.

[Human cytomegalovirus inhibits proliferation of duct epithelial cells of human salivary gland].

Objective: To investigate the effects of human cytomegalovirus (HCMV) on the proliferation of duct epithelial cells of human salivary gland (HSG).

Methods: The expression of proliferating cell nuclear antigen (PCNA) and p53 were studied in 11 cases of parotid cytomegalic inclusive disease (PCID) using immunohistochemical staining method. The effects of human cytomegalovirus (HCMV) on the proliferation of HSG were investigated by MTT method in vitro.

[Cloning of human angiogenesis inhibitor METH1 and its expression in mammalian cells].

Objective: To get the full length of human METH1 cDNA and express it steadily in mammalian cell stably.

Methods: METH1 was amplified by RT-PCR, and cloned into pCDNA3.0 after confirmed by sequence analysis.

[Inclusion bodies of human cytomegalovirus are composed of the DNA and immediately early and early antigens of the virus].

Background: To study the composition and significance of the inclusion bodies of human cytomegalovirus (HCMV).

Methods: Microdissection of inclusion bodies, PCR and Southern blot were adopted to detect DNA, and immunohistochemistry method and catalyzed signal amplification (CSA) were used to detect the different antigens of HCMV.

Results: The inclusion bodies of HCMV were separated from the tissue section of human salivary gland.