Characterization of an International High-Risk Escherichia coli ST410 Clone Coproducing NDM-5 and OXA-181 in a Food Market in China.

During a 2020 routine epidemiological investigation of carbapenem-resistant at a local food market in Guangzhou, China, two Escherichia coli ST410 isolates coproducing NDM-5 and OXA-181 were obtained from environmental samples. Antimicrobial susceptibility testing, whole-genome sequencing, and conjugation assays were applied to identify their resistance phenotypes, phylogenetic relatedness, and genetic characteristics. Phylogenetic analysis showed that the two isolates were clonally related with only one core-genome single-nucleotide polymorphism (SNP) difference and clustered into a branch with 87 E.

Clonal spread of O101: H9-ST10 and O101: H9-ST167 strains carrying and among diarrheal calves in a Chinese farm, with Australian as the possible origin of O101: H9-ST10.

During a 2018 antimicrobial resistance surveillance of isolates from diarrheal calves in Xinjiang Province, China, an unexpectedly high prevalence (48.5%) of fosfomycin resistance was observed. This study aimed to reveal the determinants of fosfomycin resistance and the underlying transmission mechanism.

[Retrieval of leaf net photosynthetic rate of moso bamboo forests using hyperspectral remote sen-sing based on wavelet transform].

This study focused on retrieval of net photosynthetic rate (Pn) of moso bamboo forest based on analysis of wavelet transform on hyperspectral reflectance data of moso bamboo forest leaf. The result showed that the accuracy of Pn retrieved by the ideal high frequency wavelet vegetation index ( VI) was higher than that retrieved by low frequency wavelet VI and spectral VI. Normalized difference vegetation index of wavelet (NDVIw), simple ratio vegetation index of wavelet (SRw) and difference vegetation index of wavelet (Dw) constructed by the first layer of high frequency coefficient through wavelet decomposition had the highest relationship with Pn, with the R² of 0.

[Retrieval of crown closure of moso bamboo forest using unmanned aerial vehicle (UAV) remotely sensed imagery based on geometric-optical model].

This research focused on the application of remotely sensed imagery from unmanned aerial vehicle (UAV) with high spatial resolution for the estimation of crown closure of moso bamboo forest based on the geometric-optical model, and analyzed the influence of unconstrained and fully constrained linear spectral mixture analysis (SMA) on the accuracy of the estimated results. The results demonstrated that the combination of UAV remotely sensed imagery and geometric-optical model could, to some degrees, achieve the estimation of crown closure. However, the different SMA methods led to significant differentiation in the estimation accuracy.

In silico identification of novel kinase inhibitors by targeting B-Raf(v660e) from natural products database.

The Ras/Raf/MEK/ERK (MAPK) signaling pathway has gained much attention from scientific community for therapeutic intervention in the past decades, specifically in oncology. Notably, a most prevalent B-Raf(v600e) mutant in Raf kinase family exhibits elevated kinase activity and results in constitutive activation of the MAPK pathway, thus making it a promising drug target for cancer therapy. Herein, virtual screening is applied to identify its potential inhibitors.