[Development of new methods for the diagnosis of nasopharyngeal carcinoma].

Objective: Development of new methods, ELISA and immunostrip test, for the diagnosis of nasopharyngeal carcinoma.

Methods: The engineering purified antigens coat plate or absorb on nitrocellulose filter. The plate and diagnostic strips carrying antigens were used for detection of IgG antibody in the sera from nasopharyngeal carcinoma patients and outpatients patients.

Preparation of monoclonal antibody against HPT and its application to detecting marker protein in genetically modified rice.

Objective: To produce the monoclonal antibodies (mAbs) against hygromycin B phosphotransferase (HPT) and to develop immunoassay based on mAbs for biosafety assessment of HPT in genetically modified rice (GM rice).

Methods: BALB/c mice were immunized with purified recombinant 6His. HPT protein, and the conventional hybridoma technology was used to generate the monoclonal hybridoma cells.

Stability of SARS coronavirus in human specimens and environment and its sensitivity to heating and UV irradiation.

Objective: The causal agent for SARS is considered as a novel coronavirus that has never been described both in human and animals previously. The stability of SARS coronavirus in human specimens and in environments was studied.

Methods: Using a SARS coronavirus strain CoV-P9, which was isolated from pharyngeal swab of a probable SARS case in Beijing, its stability in mimic human specimens and in mimic environment including surfaces of commonly used materials or in household conditions, as well as its resistance to temperature and UV irradiation were analyzed.