RNA methyltransferase NSUN2 promotes gastric cancer cell proliferation by repressing p57 by an mC-dependent manner.

The RNA methyltransferase NSUN2 has been involved in the cell proliferation and senescence, and is upregulated in various types of cancers. However, the role and potential mechanism of NSUN2 in gastric cancer remains to be determined. Our study showed that NSUN2 was significantly upregulated in gastric cancers, compared to adjacent normal gastric tissues.

The optimum marker for the detection of lymphatic vessels.

Podoplanin, lymphatic vessel endothelial hyaluronic acid receptor-1, prospero-related homeobox-1 and vascular endothelial growth factor receptor 3 have been demonstrated to have crucial roles in the development of the lymphatic system and lymphangiogenesis process by combining with their corresponding receptors. Thus, the four markers have been widely used in labelling lymphatic vessels for the detection of lymphangiogenesis and lymphatic vessel invasion. Numerous authors have aimed to identify the roles of these four markers in the lymphatic system and the mechanisms have been partly clarified at the molecular level.

[Experimental study on early pulmonary injury induced by severe acute pancreatitis in rats].

Objective: To investigate the injury to pulmonary tissue induced by severe acute pancreatitis (SAP) during its early period.

Methods: Forty adult male Sprague-Dawley (SD) rats weighing 300-400 g were randomly divided into 4 groups with 8 animals in each group: sham operation group and 30, 60, 120 and 360 minutes after SAP groups. The model of SAP was reproduced by retrograde injection of 5% sodium taurocholate into the pancreatic duct of rats.

Expression of insulin-like growth factor binding protein-2 in gastric carcinoma and its relationship with cell proliferation.

Aim: To investigate the expression of insulin-like growth factor binding protein-2 (IGFBP-2) in gastric carcinoma and its clinical significance and to explore its relationship with cell proliferation.

Methods: Expressions of IGFBP-2 and Ki-67 in 118 cases of gastric carcinoma and 40 cases of normal gastric mucosa were detected by EnVision immunohistochemical technique.

Results: Expression of IGFBP-2 in gastric carcinoma was higher than that in normal gastric mucosa (P < 0.

Relationship between inactivation of p16 gene and gastric carcinoma.

Aim: To investigate the relationship between inactivation of p16 gene and gastric carcinoma, and the mechanism of inactivation of p16 gene in gastric carcinogenesis.

Methods: 40 fresh tumor tissue specimens were taken from primary gastric cancer patients. Expression of P16 protein was detected by immunohistochemical method.

A ten-year study on non-surgical treatment of postoperative bile leakage.

Aim: To summarize systematically our ten-year experience in non-surgical treatment of postoperative bile leakage, and explore its methods and indications.

Methods: The clinical data of 57 patients with postoperative bile leakage treated non-surgically from January 1991 to December 2000 were reviewed retrospectively.

Results: The site of the leakage was mainly the disrupted or damaged fistulous tracts of T tube in 25 patients (43.