Analysis of the heavy metal contents' effect on steroidal saponins and the anti-breast cancer activity of var. .

var. , as a near-threatened and ethnic medicine in China, used to be a key ingredient in traditional Chinese medicine in treatment of traumatic injuries, sore throat, snakebites, and convulsions for thousands of years. However, there were no reports on the inverse relationship between the contents of heavy metals and saponins and its anti-breast cancer pharmacological activity in var.

Characterization and analysis of complete chloroplast genome of (Blanco) Merrill.

In this study, the chloroplast genome (Cp) of ((Blanco) Merrill) was sequenced by high-throughput sequencing technology. We found that the length of the chloroplast genome of was 159,753 bp, and the total GC content was 38.7%, including a large single-copy (LSC) region of 87,623 bp, a small single-copy (SSC) region of 17,986 bp, and 27,072 bp pairs of reverse repeats (IRS).

Complete chloroplast genome sequence and phylogenetic analysis of Franch.

Franch. is a Chinese unique medicinal plant with high value for decoration, human consumption, and as a medicine. In this study, we sequenced the complete chloroplast (cp) genome of Franch.

The complete chloroplast genome and phylogenetic analysis of matsuda (Malvaceae).

Matsuda is an economically and medicinally important plant. Here, we report the first chloroplast (cp) genome of the genus (). The complete cp genome is 159,878 bp in length with an overall GC content of 36.

The complete chloroplast genome sequences and phylogenetic analysis of (Leguminosae).

The complete chloroplast genome of was obtained using the high-throughput sequencing technology in this article. The complete chloroplast genomes of this species were 152,658 bp in length, consisting of a large single-copy region (LSC) of 83,652 bp and a small single-copy region (SSC) of 18,028 bp, which were separated by a pair of inverted repeat (IRs) regions of 25,489 bp. The chloroplast genome contained 111 unique genes, including 77 protein-coding genes, 30 genes, and four genes.

[Screening and identification of potassium-dissolving bacteria from different rhizosphere soil of Paris polyphylla var. yunnanensis].

The study aiming at exploring the potassium-dissolving capacity of rhizosphere potassium-dissolving bacteria from diffe-rent sources and screen the strains with high potassium-dissolving ability, so as to lay a theoretical foundation for cultivation and quality improvement of Paris polyphylla var. yunnanensis sources. The rhizosphere soil of 10 wild and transplanted species from Yunnan, Sichuan and Guizhou provinces was used as the research object.

[Isolation and identification of phosphatolytic bacteria in Paris polyphylla var. yunnanensis].

The wild resources of Paris polyphylla var. yunnanensis, a secondary endangered medicinal plant, are severely scarce. Introduction and cultivation can alleviate market demand.

The complete chloroplast genome of DC. (Rutaceae) and its phylogenetic analysis.

We sequenced the complete chloroplast genome of via high-throughput sequencing technology, and analyzed its structural characteristics and phylogenetic relationships. The chloroplast exhibits a genome length of 158,473 bp, including a pair of inverted repeat regions (IRa and IRb) of 27,369 bp, a small single-copy (SSC) region of 17,629 bp and a large single-copy (LSC) region of 85,570 bp. The annotation analysis identified 112 genes, containing 78 protein-coding genes, 30 tRNA genes and 4 rRNA genes.

[Correlation analysis of quality,origin and phenotypic characters of Paris polyphylla var. yunnanensis].

In order to provide guidance for the protection and utilization of resources,quality control and breeding of improved varieties,we compared the main phenotypic characters and quality of wild and transplanted Paris polyphylla var. yunnanensis collected from different producing areas. Seven phenotypic characters of 33 samples of P.

[Correlation between rhizospheric microorganisms distribution and alkaloid content of Fritillaria taipaiensis].

In order to reveal the relationship between the amount of soil microorganisms and the quality of Fritillaria taipaiensis, both cultivated and wild F. taipaiensis were collected from Chongqing, Wuxi at different stages of their growth as objects of the research. The mycorrhizal infection rate and colonization intensity, peimisine and total alkaloid content in bulbs, the amount of microorganisms and biomass carbon content in rhizospheric soil were all determined using common methods.

[Effects of Different AM Fungi on Quantity and Enzyme Activity of Rhizosphere Soil Microorganism of Paris polyphylla var. yunnanensis].

Objective: To investigate the rhizosphere soil microorganism and enzyme activity after Paris polyphylla var. yunnanensis inoculated Arbuscular Mycorrhizal( AM) fungi,which provide the technological condition for artificial cultivation of Paris polyphylla var. yunnanensis.

[Photosynthetic parameters and physiological indexes of Paris polyphylla var. yunnanensis influenced by arbuscular mycorrhizal fungi].

Through potted inoculation test at room temperature and indoor analysis, the photosynthetic parameters and physiological and biochemical indexes of Paris polyphylla var. yunnanensis were observed after 28 arbuscular mycorrhizal (AM) fungi were injected into the P. polyphylla var.

[Effects of mycorrhizal colonization and medicine quality of Paris polyphylla var. yunnanensis inoculated by different foreign AM fungi species].

After 28 foreign species of AM fungi were inoculated in sterilized soil, the effects of the AM mycorrhizal colonization and the medicine quality of Paris polyphylla var. yunnanensis were observed by combination of inoculation test in pot at room temperature and instrumental analysis. The results showed that, compared with control group (CK), the inoculation of foreign AM fungi in the soil influenced the spore density, mycorrhizal infection rate, and colonization intensity of AM fungi in root system of P.

[Identification of Active Composition and Quality Evaluation of Paris Herbs Artificially Cultivated in Different Locations of Three Gorges Reservoir].

Objective: In order to apply the thesis basis for screening the Paris varieties which was suitable for the environments, the quality of Paris herbs of different varieties cultivated in different locations of Three Gorges Reservoir was evaluated based on the identification of active compositions.

Methods: The content of saponins in Paris herbs was measured by HPLC method; The total content of saponins, the total content of flavonoids and the total content of polysaccharides were evaluated using ultraviolet-visible spectrophotometry; Then the contents of active chemical compositions were used for comparison of the Paris herbs of different varieties in different locations.

Results: Types and the contents of saponin I, saponin II, saponin VI and saponin VII were all various in different Paris varieties located in different locations; with the analyses of the total content of saponins, the total content of flavonoids and the total content of polysaccharides, it was suggested that the chemical compositions existed obvious differences in different cultivars.

[Correlation between distribution of rhizospheric microorganisms and contents of steroidal saponins of Paris polyphylla var. yunnanensis].

In this paper, the varying pattern of the amount of rhizospheric microorganisms, including bacteria, actinomycetes and fungus, was observed during the cultivation of Paris polyphylla var. yunnanensis. And the correlations between number of rhizospheric microorganisms and the quality of P.

Tamsulosin alters levofloxacin pharmacokinetics in prostates derived from rats with acute bacterial prostatitis.

The combination of levofloxacin and α1 adrenergic antagonist treatment is the current preferred choice for both bacterial and non-bacterial prostatitis. The aim of this study is to explore the influence of α1 adrenergic antagonists on the pharmacokinetics of levofloxacin using rat models with acute bacterial prostatitis (ABP) induced by direct injection with Escherichia coli (ATCC25922). A total of 96 model rats were randomly assigned into two groups: the experimental group (treated with both tamsulosin and levofloxacin, n=48) and the control group (treated with levofloxacin and solvents, n=48).

[Present status of clinical researches on the screening for prostate cancer biomarkers].

Researches on the biomarkers of tumors are very important for their early diagnosis and treatment. Although PSA has been used for over 20 years for screening prostate cancer and evaluating its treatment, the results are not entirely satisfactory. With the deepening of the researches on prostate cancer, a variety of prostate cancer biomarkers have been found.