MiR-708-5p/Pit-1 axis mediates high phosphate-induced calcification in vascular smooth muscle cells via Wnt8b/β-catenin pathway.

Recently, the underlying mechanism of vascular calcification (VC) has been partially elucidated. However, it is still high incidence, and no effective treatment has been found. This study aims at figuring out the underlying mechanisms of microRNA-708-5p (miR-708-5p)/sodium-phosphate transporter 1 (Pit-1) axis in high phosphate (HP)-induced VC of T/G HA-VSMCs.

[Endometrial adenocarcinoma and clear cell carcinoma in a young woman with polycystic ovarian syndrome: a case report].

A 26-year-old unmarried woman with irregular menstruation for 4 years was admitted for an intrauterine space-occupying mass. Pathological examination before surgery showed moderately to poorly differentiated endometrial adenocarcinoma. The patient underwent laparoscopically assisted epifascial panhysterectomy with bilateral salpingo-oophorectomy.

[MiR-135b promotes proliferation of endometrial carcinoma cells by targeting FOXO1].

Objective: To explore the expression of miR-135b in endometrial carcinoma and the mechanism by which miR-135b promotes the proliferation of endometrial cancer cells.

Methods: The expressions of miR-135b and FOXO1 were using RT-PCR detected in 22 fresh endometrial cancer tissues and paired adjacent tissues and also in endometrial cancer cell lines JEC, Ishikawa, HEC-1-B, and RL-952. The RL-952 and Ishikawa cell lines were transfected with miR-135b mimics or inhibitors, and the changes in their proliferative activity were detected with MTT assay; the expressions of FOXO1 mRNA and protein were detected by RT-PCR and Western blotting, respectively.

[Study of integrated state of HPV-16 infection in cervical cancer and precancerous tissues].

Objective: To investigate the prevalence of physical state of HPV-16 DNA in cervical cancer and cervical precancerous carcinoma.

Methods: Multiplex PCR was adopted to detect the physical state of HPV in samples from 252 patients with cervical carcinoma, including 48 samples of cervical cancer, 204 cervical intraepithelial neoplasia (CIN ) (125 CIN I, 46 CIN II and 33 CIN III) and 20 normal samples from the subjects with hysteromyoma undergoing hysterectomy, respectively.

Results: Among 48 patients with cervical cancer, 31 (65.

Preoperative CT findings of thymoma are correlated with postoperative Masaoka clinical stage.

Rationale And Objectives: Both preoperative computed tomography (CT) staging and postoperative surgical Masaoka clinical staging are of great clinical importance for diagnosing thymomas. Our study aimed to investigate the relationships between these two staging systems.

Materials And Methods: This was a retrospective review of 129 patients who had undergone thymoma surgery.

Primary retroperitoneal malignant melanoma: A case report.

Primary malignant melanoma occurring at an extra cutaneous site is rare. A case of primary malignant melanoma located in the retroperitoneum of an 18-year-old female is presented in this study. Histopathological examination of the tissue biopsies at laparotomy with immunohistochemical stains confirmed a diagnosis of malignant melanoma.

[Long-term culture and identification of CD133⁺ hematopoietic progenitor cells from human umbilical cord blood].

Objective: To isolate CD133(+) hematopoietic progenitor cells from human umbilical cord blood and optimize the culture condition for maintaining their stem cell characteristics.

Methods: CD133(+) hematopoietic progenitor cells were isolated from human umbilical cord blood using magnetic cell sorting system, and the cells were detected by flow cytometry. Four methods were used for culturing cells.

[Construction of has-miR-335 lentiviral vector and verification of the target gene of miR-335].

Objective: To construct a lentiviral vector of miR-335 gene and verify the target gene of miR-335.

Methods: The precursor sequence of miR-335 gene was amplified from the genomic DNA by PCR and cloned into the lentiviral vector PLVTHM labeled with GFP. Real-time quantitative RT-PCR was used to detect miR-335 and RASA1 expression in different colorectal cancer cell lines.

[Touchdown PCR and overlap extension PCR for generating CD133(+) cancer stem cell-selective adenovirus vector].

Objective: To construct a replication-incompetent adenovirus vector targeting cancer stem cells by modified touchdown PCR and overlap extension PCR and investigate its infection efficiency in CD133(+) SW480 cells in vitro.

Methods: The two portions of the fiber gene encoding the Ad5 fiber knob domain with the HI loop deleted were amplified using two pairs of designed primers and then linked by overlap extension PCR. The product obtained was identified by sequencing and inserted into prokaryotic expression vector pEGFP-N1.

[Selection and identification of specific-binding peptides for cancer stem cell surface marker CD133].

Objective: To select the peptides that specifically bind human cancer stem cell surface marker CD133 from the Ph.D.-7>(TM) phage peptide library.

[Randomized comparative study on the lipid lowering effects of Zhikang Granule and simvastatin].

Objective: To compare the lipid lowering effects of Zhikang Granule (ZKG) and simvastatin.

Methods: Forty-five out-patients with hyperlipemia who met the entry criteria were enrolled and randomized into two groups in the ratio of 2: 1, 30 patients in the ZKG group and 15 patients in the simvastatin group. The lipid lowering effects and safety of treatment during the 24-week therapeutic period, as well as the influence of treatment on plasma high sensitivity C reactive protein (hs-CRP) level in patients were observed.

Human telomerase RNA gene amplification detection increases the specificity of cervical intraepithelial neoplasia screening.

Objectives: The purpose of this study was to evaluate the clinical significance of genomic amplification of the human telomerase RNA gene (TERC) for cervical cancer screening and explore whether it can serve as a biomarker to improve the specificity of human papillomavirus (HPV) DNA testing for cervical cancer screening.

Methods: One hundred twenty women, including 20 cases of normal (control), 14 cases of cervical intraepithelial neoplasia grade I (CIN I), 35 cases of CIN II, 36 cases of CIN III, and 15 cases of squamous cervical cancer diagnosed by histopathologic evaluation, were subjected to cytopathologic examination, TERC detection by fluorescence in situ hybridization (FISH), and HPV DNA testing by Hybrid Capture II.

Results: TERC amplification was significantly associated with cytopathologic diagnosis (P < 0.

[Proteinchip-flight mass spectrometry for screening differentially expressed proteins in the serum of cervical cancer patients].

Objective: To identify the differentially expressed proteins in the serum of patients with cervical cancer for use as the biomarkers for early diagnosis of cervical cancer.

Methods: Surface-enhanced laser desorption/ionization time of flight mass spectrometry (SELDI-TOF-MS) with weak cationic chips (CM10) was used to examine the serum samples of 24 patients with cervical squamous cell carcinoma and 25 age-matched healthy women. The protein fingerprints were obtained, and bioinformatic analysis was performed to identify the differentially expressed proteins in the serum of the patients.

[Correlation between human papillomavirus DNA in the lymph nodes and metastasis of early-stage cervical carcinoma].

Objective: To investigate the correlation between the presence of human papillomavirus (HPV) DNA in the lymph nodes and histopathologically confirmed metastasis of early-stage cervical carcinoma.

Methods: HPV L1 gene fragment in paraffin-embedded tissue specimens of the primary tumor and pelvic lymph nodes from 31 patients with cervical cancer was amplified using HPV-specific PCR with general consensus primers GP5+/GP6+. The type of HPV was identified by sequence analysis of the PCR products, and the correlation between the presence of HPV DNA in the lymph node and the clinicopathological indices of cervical carcinoma was analyzed.

[Preliminary study of gene expression profiling in human type I and II endometrial carcinoma].

Objective: To study gene expression profiling in human type I and II endometrial carcinoma.

Methods: Six Affymetrix human genome genechips were utilized to investigate the differences in gene expression profiles between type I and II endometrial carcinoma with bioinformatic analysis.

Results: Many genes were highly expressed in estrogen-dependent endometrial carcinoma, and some of them were involved in the metabolism and conversion of estrogen, while some others in estrogen regulation.

[Sequence polymorphism of human papillomavirus type 16 E7 in cervical cancer].

Objective: To explore the genetic polymorphism of E7 open-reading frame of human papillomavirus (HPV) type 16 in cervical cancer.

Methods: The types of HPV was identified by sequence analysis of the PCR product of HPV in cervical cancer tissues. HPV 16 gene fragment in the cervical cancer tissue was amplified by HPV-specific PCR with general consensus primers.

[Significance of pelvic lymph node human papillomavirus DNA detection in cervical cancer].

Objective: To assess the significance of human papillomavirus (HPV) DNA detection in the pelvic lymph nodes in cervical cancer.

Methods: HPV L1 gene fragment was amplified by HPV-specific PCR with general consensus primers from cervical cancer tissues. The types of HPV were identified by sequencing of the PCR product.

[Detection of human papillomavirus DNA in cervical cancer tissue by PCR and direct sequencing].

Objective: To explore an effective method for detecting human papillomavirus (HPV) DNA in cervical cancer tissue.

Methods: HPV L1 gene fragment in cervical cancer tissue was amplified by HPV-specific PCR with consensus primers, and typing of the HPV strains was performed on the basis of sequence analysis of the PCR product.

Results: The positivity rates of HPV DNA was 78% in the 50 cases of cervical cancer, and mixed infection with HPV16 and HPV18 strains was the most common, which accounted for 48% on the total infections.

Selection of choriocarcinoma-associated genes using bioinformatics.

Objective: To analyze the data of gene expression profiles of choriocarcinoma and screen for choriocarcinoma- related genes.

Methods: Human cDNA expression microarray containing 4 096 genes was used to study the gene expression profiles in specimens of complete hydatidiform moles (n=3) and choriocarcinomas (n=3), with normal placental villi serving as the control group. The candidate genes with similar expression profiles were identified by hierarchical cluster analysis, and their expressions in normal and neoplastic tissues analyzed by electronic Northern analysis and other bioinformatics methods.

[Age and prognosis: analysis of their association in 93 cases of cervical cancer].

Objective: To investigate the relationship between the patient's age and clinical prognosis of cervical cancer.

Methods: Ninety-three patients with cervical cancer selected from those hospitalized in Nanfang Hospital from Jan 1997 to Dec 2002 were divided into younger age (less than 35 years old) and elder age group ( more than 35 years old). Comparison was made in the clinical stages, pathological grade and differentiation between the 2 groups.

[Comparative study of the impacts of laparoscopic and open laparotomic surgeries on oxidative stress in patients with uterine myomas].

Objective: To evaluate the impact of laparoscopic surgery on oxidative stress in patients with uterine myomas.

Methods: Plasma malondialdehyde (MDA), antioxidant activity (AOA), and glutathione peroxidase (GPx) activity in 20 patients with uterine myomas were measured before, at the end of (5 min after deflation), and 24 h after laparoscopic surgery, for comparison with the measurements in 20 patients receiving laparotomic surgery at the same time points.

Results: Immediately following laparoscopic surgery, plasma MDA level increased significantly as compared with the preoperative level (P<0.

Transcriptional gene expression profiles of HGF/SF-met signaling pathway in colorectal carcinoma.

Aim: To explore the transcriptional gene expression profiles of HGF/SF-met signaling pathway in colorectal carcinoma to understand mechanisms of the signaling pathway at so gene level.

Methods: Total RNA was isolated from human colorectal carcinoma cell line LoVo treated with HGF/SF (80 ng/L) for 48 h. Fluorescent probes were prepared from RNA labeled with cy3-dUTP for the control groups and with cy5-dUTP for the HGF/SF-treated groups through reverse-transcription.