Publications by authors named "Guo Zhong Chen"

Article Synopsis
  • Safer and more effective treatments for acute pancreatitis (AP) are needed, and Qingjie Huagong decoction (QJHGD) has shown promising clinical effects but its mechanisms were unclear.
  • The study aimed to explore how QJHGD influences AP both in cell cultures and in mouse models, using various biochemical and molecular techniques to analyze its effects.
  • Results indicated that QJHGD enhances cell viability and reduces pancreatic injury by regulating a specific pathway involving circHipk3, miR-193a-5p, and NLRP3, which highlights a new mechanism for its therapeutic action against AP.
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  • The study focuses on how certain bacteria adapt to their host’s gut environment by changing their gene expression, particularly in fully colonized adult mice.
  • Through the innovative use of coated-magnetic chitin beads (vcMCB), the researchers successfully extracted high-quality RNA, allowing for a detailed analysis of gene expression differences between bacteria from adult mice and those from infant models.
  • The findings highlight a specific bacterial strain's ability to survive low pH and formic acid by altering its gene expression, offering new insights into the relationship between bacteria and gut microbiota, which can inform future research on other pathogenic bacteria.
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  • The study aimed to identify the key targets and mechanisms of Dahuang-Danshen (DH-DS) in treating acute pancreatitis (AP) using network pharmacology and animal experiments.
  • Sixty-seven common targets were found, with MAPK3, JAK2, STAT3, and FOS highlighted as core targets, while gene ontology and pathway analyses identified cellular response and Th17 cell differentiation as main mechanisms.
  • Animal tests confirmed that DH-DS effectively reduced inflammation and protected pancreatic tissue by inhibiting specific inflammatory markers and regulating Th17 cell differentiation.
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  • A significant study integrated transcriptome data from both RNA-seq and microarray platforms for Vibrio cholerae, marking the first effort to combine these two types of data.
  • By analyzing the integrated data, researchers identified key gene expressions and functional modules related to stress responses, gene manipulation, and metabolic pathways.
  • The study also revealed novel protein interactions and highlighted similarities between clinical samples and experimental conditions, providing valuable insights into V. cholerae's pathogenic mechanisms.
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Due to the increasing use of local anesthetic techniques in various healthcare settings, local anesthetic toxicity still occurs. Seizures are the most common symptom of local anesthetic toxicity. The relationship between local anesthetic-induced seizures and the sensation of pain has not been established till now.

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Marine-derived fungi are a treasure house for the discovery of structurally novel secondary metabolites with potential pharmaceutical value. In this study, a pair of new nor-bisabolane derivative enantiomers (±)-1 and two new phthalides (4 and 5), as well as four known metabolites, were isolated from the culture filtrate of the marine algal-derived endophytic fungus LD-201810. Their structures were established by detailed interpretation of spectroscopic data (1D/2D NMR and ESI-MS).

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Fungi play an irreplaceable role in drug discovery in the course of human history, as they possess unique abilities to synthesize diverse specialized metabolites with significant medicinal potential. are well-studied filamentous fungi generally observed in nature, which are widely marketed as biocontrol agents. The secondary metabolites produced by have gained extensive attention since they possess attractive chemical structures with remarkable biological activities.

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Neuropathic pain is a common chronic pain condition with major impact on quality of life. However, its physiopathologic mechanism remains unknown and pain management is still a challenge. Accumulating evidence indicated that C-X-C chemokine receptor type 4 (CXCR4) played a critical role in the process of pain.

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Ulinastatin is a broad-spectrum protease inhibitor widely used for the treatment of various inflammation-related diseases owing to its recognized excellent anti-inflammatory and cytoprotective properties. However, whether ulinastatin can relieve postoperative pain remains unclear. In this study, we evaluated the analgesic effects of ulinastatin administered either as a single agent or in combination with sufentanil in a validated preclinical rat model of postoperative pain induced by plantar incision.

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Introduction: Ulinastatin, a broad-spectrum serine protease inhibitor, has been widely used to treat various diseases clinically. However, so far, the antinociceptive effect of ulinastatin remains less studied experimentally and the underlying mechanisms of ulinastatin for pain relief remain unclear. This study aimed to find evidence of the analgesic effect of ulinastatin on acute somatic and visceral pain.

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Objectives: To evaluate the relationship between hemodynamics and vessel wall remodeling patterns in middle cerebral artery (MCA) stenosis based on high-resolution magnetic resonance imaging and computational fluid dynamics (CFD).

Methods: Forty consecutive patients with recent ischemic stroke or transient ischemic attack attributed to unilateral atherosclerotic MCA stenosis (50-99%) were prospectively recruited. All patients underwent a cross-sectional scan of the stenotic MCA vessel wall.

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Background: Over-expression of spinal protein kinase Cγ(PKCγ) contributes to the induction of persistent bilateral hyperalgesia following inflammatory injury, yet the role of spinal PKCγ in short- and long-lasting pain behavior is poorly understood.

Objective: This study aimed to characterize the contribution of spinal PKCγ to spontaneous pain and long-lasting bilateral hyperalgesia in formalin-induced inflamed mice using pharmacological inhibition.

Study Design: Laboratory animal study.

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This work investigated the cultivation of Chlorella vulgaris in a thin-film flat plate photobioreactor under outdoor conditions and using digested piggery wastewater as the culture medium. The algal cells were able to adapt quickly to the wastewater and outdoor conditions. A specific growth rate of 0.

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Acute lung injury (ALI) is the common and complicated inflammatory lung disease. MicroRNAs (miRNA) have emerged as novel gene regulatory molecules which play a crucial role in multiple complicated diseases, including ALI. In this study, we aims to identify potential regulatory functions of miRNA-1246 in lipopolysaccharide (LPS)-induced ALI.

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Objective: Remote ischemic postconditioning (RIPostC) has been recognized as an applicable strategy for protecting against cerebral ischemia/reperfusion (I/R) injury. This study was performed to examine the effect of RIPostC on cerebral I/R and to explore its underlying mechanism.

Methods: Healthy male SD rats (N = 36) were assigned randomly into 3 groups of 12 each: sham group, I/R model group and RIPostC group.

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Objective: To evaluate the diagnostic accuracy of digital subtraction CT angiography (DS-CTA) in detecting posterior inferior cerebellar artery (PICA) aneurysms with digital subtraction angiography (DSA) as reference standard.

Methods: A total of 115 patients, including 56 patients diagnosed with PICA aneurysms by CTA or DSA and 59 non-PICA-aneurysm patients were included in this retrospective study. All patients underwent DS-CTA and DSA.

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Objectives: To evaluate the radiation dose and image quality of cerebral CT angiography (CTA) at 70kVp with 30mL iodinated contrast agent.

Methods: One hundred patients were prospectively classified into two groups: Group A (n=50), 120kVp cerebral CTA with 60mL iodinated contrast agent reconstructed by filtered back projection (FBP) and Group B (n=50), 70kVp with 30mL iodinated contrast agent reconstructed by sinogram-affirmed iterative reconstruction (SAFIRE). CT values, noise, signal-to-noise ratio (SNR) and contrast-to-noise ratio (CNR) of the internal carotid artery (ICA) and middle cerebral artery (MCA) were measured.

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Background: Sevoflurane presents reliable central neuromuscular effects. However, little knowledge is available regarding the interaction between sevoflurane and demedetomidine. We evaluated the neuromuscular effect of dexmedetomidine on sevoflurane in patients with normal neuromuscular transmission and calculated the 50% effective concentration (EC50).

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A Gram-stain-negative, aerobic, yellow-pigmented, non-flagellated, non-gliding, oxidase- and catalase-positive bacterium, designated CY01T, was isolated from seawater of the Yellow Sea. CY01T grew at 15-37 °C (optimum, 30 °C), pH 5-8 (optimum, 6.5-7.

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Objectives: This study aimed to intra-individually and inter-individually compare image quality, radiation dose, and diagnostic accuracy of dual-source dual-energy computed tomography pulmonary angiography (CTPA) protocols in patients with suspected pulmonary embolism (PE).

Methods: Thirty-three patients with suspected PE underwent initial and follow-up dual-energy CTPA at 80/Sn140 kVp (group A) or 100/Sn140 kVp (group B), which were assigned based on tube voltages. Subjective and objective CTPA image quality and lung perfusion map image quality were evaluated.

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Aneurysmal subarachnoid hemorrhages have extremely high case fatality in clinic. Early and rapid identifications of ruptured intracranial aneurysms seem to be especially important. Here we evaluate clinical value of single phase contrast-enhanced dual-energy CT angiograph (DE-CTA) as a one-stop-shop tool in detecting aneurysmal subarachnoid hemorrhage.

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Background: Reconstruction of patient-specific biomechanical model of intracranial aneurysm has been based on different imaging modalities. However, different imaging techniques may influence the model geometry and the computational fluid dynamics (CFD) simulation. The aim of this study is to evaluate the differences of the morphological and hemodynamic parameters in the computational models reconstructed from computed tomography angiography (CTA), magnetic resonance angiography (MRA) and 3D rotational angiography (3DRA).

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Purpose: To evaluate image quality and diagnostic accuracy for acute infarct detection and radiation dose of 70 kVp whole brain CT perfusion (CTP) and CT angiography (CTA) reconstructed from CTP source data.

Methods: Patients were divided into three groups (n = 50 each): group A, 80 kVp, 21 scanning time points; groups B, 70 kVp, 21 scanning time points; group C, 70 kVp, 17 scanning time points. Objective and subjective image quality of CTP and CTA were compared.

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Background: Intraoperative blood pressure (BP) is one of the basic vital signs monitoring. Compared with standard invasive BP measurement, TL-300 allows for a continuous and beat-to-beat noninvasive intraoperative BP monitoring. The current retrospective study compared the accuracy and precision of this noninvasive technique for continuous BP monitoring with that of standard invasive BP measurement in patients undergoing elective neurosurgery.

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