CTB6 Confers Cold Tolerance at the Booting Stage by Maintaining Tapetum Development in Rice.

Rice is highly sensitive to cold stress, particularly at the booting stage, which significantly threatens rice production. In this study, we cloned a gene, CTB6, encoding a lipid transfer protein involved in cold tolerance at the booting stage in rice, based on our previous fine-mapped quantitative trait locus (QTL) qCTB10-2. CTB6 is mainly expressed in the tapetum and young microspores of the anther.

Effect of gestational age on clinical features in necrotizing enterocolitis-associated intestinal perforation.

Purpose: To investigate the clinical features of necrotizing enterocolitis-associated intestinal perforation (NEC-IP) in neonates with different gestational ages (GAs). Furthermore, we also want to identify the risk factors of poor prognosis for these patients.

Methods: The retrospective study of patients with NEC-IP was conducted with basic information, comorbidity, intraoperative findings, related treatment, and prognosis.

Predicting postoperative adhesive small bowel obstruction in infants under 3 months with intestinal malrotation: a random forest approach.

Objective: This study aimed to develop a predictive model using a random forest algorithm to determine the likelihood of postoperative adhesive small bowel obstruction (ASBO) in infants under 3 months with intestinal malrotation.

Methods: A machine learning model was used to predict postoperative adhesive small bowel obstruction using comprehensive clinical data extracted from 107 patients with a follow-up of at least 24 months. The Boruta algorithm was used for selecting clinical features, and nested cross-validation tuned and selected hyper-parameters for the random forest model.

Single-incision Laparoscopic Surgery for Neonatal Congenital Duodenal Obstruction: A Retrospective Study of 130 Patients.

Objectives: This study sought to assess the advantages of utilizing the single-incision laparoscopic surgery (SILS) procedure for repairing neonatal congenital duodenal obstruction (CDO) in terms of clinical outcomes and complication rates.

Methods: In a retrospective cohort study conducted at a single center, neonates with CDO who underwent SILS were compared to those who underwent conventional laparoscopic surgery (CLS) between January 2018 and December 2022. The demographic and operative characteristics of CDO patients who underwent SILS or CLS were analyzed, including conversion rates and postoperative complications.

Risk factors and simple scoring system for predicting postoperative nutritional status of Hirschsprung's disease.

Background: Nutritional problem after surgery for Hirschprung's disease (HSCR) was not optimistic. This study aimed to analyze the risk factors of postoperative undernutrition for patients with HSCR and establish a scoring system for predicting postoperative undernutrition.

Methods: Retrospective review of 341 patients with HSCR who received Laparoscopic-assisted pull-through surgery in a tertiary-level pediatric hospital was conducted with assessments of clinical data.

A Scalable Pore-space-partitioned Metal-organic Framework Powered by Polycatenation Strategy for Efficient Acetylene Purification.

Efficient separation of acetylene (CH) from carbon dioxide (CO) and ethylene (CH) is a significant challenge in the petrochemical industry due to their similar physicochemical properties. Pore space partition (PSP) has shown promise in enhancing gas adsorption capacity and selectivity by reducing pore size and increasing the density of guest binding sites. Herein, we firstly employ the 2D→3D polycatenation strategy to construct a PSP metal-organic framework (MOF) Ni-dcpp-bpy, incorporating functional N/O sites to enhance CH purification.

Haplotype-resolved nonaploid genome provides insights into flowering in bamboos.

Woody bamboos (Bambusoideae) are renowned for its polyploidy and rare flowering. is one of the bamboo species with the highest chromosome count (104) in the subfamily and has the highest heterozygosity of all sequenced bamboo genomes so far. Compared with other bamboo species, it can efficiently utilize exogenous hormones to regulate flowering, providing valuable insights into the hormonal regulation of bamboo flowering.

Precise location of three novel linear epitopes using the generated monoclonal antibodies against the Knob domain of FAdV-4 surface structural protein, fiber1.

Background: Fowl adenovirus serotype 4 (FAdV-4) is the main pathogen of hepatitis-hydropericardium syndrome (HHS), which brings huge economic losses to the poultry industry worldwide. Fiber-1 protein plays an important role in viral infection and pathogenesis by binding directly to cellular receptors of FAdV-4. In particular, the knob domain of fiber-1 protein has been reported to induce the production of neutralizing antibodies and arouse protection against the lethal challenge of chickens with FAdV-4.

Cdon is essential for organ left-right patterning by regulating dorsal forerunner cells clustering and Kupffer's vesicle morphogenesis.

and are members of the cell adhesion molecule subfamily III Ig/fibronectin. Although they have been reported to be involved in muscle and neural development at late developmental stage, their early roles in embryonic development remain unknown. Here, we discovered that in zebrafish, , but not , is expressed in dorsal forerunner cells (DFCs) and the epithelium of Kupffer's vesicle (KV), suggesting a potential role for in organ left-right (LR) patterning.

Leptin A deficiency affecting the mitochondrial dynamics of aged oocytes in medaka (Oryzias latipes).

Mitochondrial dysfunction and metabolic disorder have been associated to age-related subfertility, however, the precise molecular mechanism controlling the development of fertile oocytes in aging females remains elusive. Leptin plays an important role in the maintenance of energy homeostasis, as both excessive or insufficient levels can affect the body weight and fertility of mice. Here, we report that leptin A deficiency affects growth and shortens reproductive lifespan by reducing fertility in medaka (Oryzias latipes).

Early intervention in Hirschsprung's disease: effects on enterocolitis and surgical outcomes.

Background: The timing of surgical intervention for Hirschsprung's disease (HSCR) has been a topic of continued discussion. The objective of this study was to evaluate the significance of age at surgery in the management of HSCR by conducting a comparative analysis of the correlation between surgical age and midterm outcomes.

Methods: We conducted a retrospective analysis of children with HSCR who underwent one-stage laparoscopic assisted pull-through surgery with modified Swenson technology at our hospital between 2015 and 2019.

Effect of SNPs on Litter Size in Swine.

Although sows do not directly enter the market, they play an important role in piglet breeding on farms. They consume large amounts of feed, resulting in a significant environmental burden. Pig farms can increase their income and reduce environmental pollution by increasing the litter size (LS) of swine.

The Potential Value of Mean Platelet Volume and Platelet Distribution Width as Inflammatory Indicators in Surgical Necrotizing Enterocolitis.

Background: This study aims to investigate the potential significance of mean platelet volume (MPV) and platelet distribution width (PDW) in predicting surgical neonatal necrotizing enterocolitis (NEC) and establish the correlation between MPV/PDW levels and the severity/prognosis of NEC.

Methods: A retrospective study was conducted on a cohort of 372 patients diagnosed with NEC. The patients were categorized into two groups based on whether they underwent surgical therapy.

Comparative Metabolome Profiling for Revealing the Effects of Different Cooking Methods on Glutinous Rice Longjing57 ( L. var. ).

Glutinous rice (GR), an important food crop in Asia, provides prolonged energy for the human body due to its high amylopectin content. The non-volatile metabolites generated by different cooking methods that affect the nutritional value and color of GR are still poorly understood. Herein, a widely targeted metabolomics approach was used to understand the effects of different cooking methods (steaming, baking, and frying) on the metabolite profiles of GR.

Impacts of farming activities on carbon deposition based on fine soil subtype classification.

Introduction: Soil has the highest carbon sink storage in terrestrial ecosystems but human farming activities affect soil carbon deposition. In this study, land cultivated for 70 years was selected. The premise of the experiment was that the soil could be finely categorized by subtype classification.

miR-146b-5p Regulates the Enteric Nervous System Development in Hirschsprung Disease via Targeting RET Pathway.

The objective of the study is to investigate how miR-146b-5p might contribute to the etiology of HSCR. The study investigated the expression levels of miRNA, mRNA, and proteins in colon tissues obtained from the HSCR and control groups. The role of miR-146b-5p in cell proliferation and migration was studied in vitro.

Deep genome skimming reveals the hybrid origin of (Poaceae: Bambusoideae).

(Poaceae: Bambusoideae) is a temperate woody bamboo species endemic to South-central China with a narrow distribution. Previous phylogenetic studies revealed an unexpected, isolated phylogenetic position of . Here we conducted phylogenomic analysis by sampling populations of and its sympatric species and reflecting different genomic signals, by deep genome skimming.

Network Meta-Analysis: Effect of Cold Stress on the Gene Expression of Swine Adipocytes , , , and .

Cold stress significantly affects gene expression in adipocytes; studying this phenomenon can help reveal the pathogeneses of conditions such as obesity and insulin resistance. Adipocyte triglyceride lipase (); cell death-inducing deoxyribonucleic acid (DNA) fragmentation factor subunit alpha (DFFA)-like effector (); and uncoupling protein genes , , and are the most studied genes in pig adipose tissues under cold stress. However, contradictory results have been observed in gene expression changes to and when adipose tissues under cold stress were examined.

Unveiling the microbiota-metabolite-myocardium axis: a novel perspective on cardiovascular health.

Introduction: Cardiovascular diseases, including myocardial infarction, remain a leading cause of death globally. Emerging evidence suggests the gut microbiota plays a crucial role in cardiovascular health. This study aims to explore the impact of gut microbiota on myocardial infarction using a mouse model.

Difference in the Effect of Applying to Control Tomato Verticillium Wilt in Black and Red Soil.

In practical applications, the effectiveness of biological control agents such as is often unstable due to different soil environments. Herein, we aimed to explore the control effect and intrinsic mechanism of in black soil and red soil in combination with tomato Verticillium wilt. application effectively controlled the occurrence of Verticillium wilt in red soil, reducing the incidence by 19.

Melatonin's effect on hair follicles in a goat () animal model.

Introduction: Melatonin can treat androgenetic alopecia in males. Goats can be used as animal models to study melatonin treatment for human alopecia. In this study, a meta-analysis of melatonin's effects on goat hair follicles was pursued.

Clinical characteristics and influence of postoperative Hirschsprung-associated enterocolitis: retrospective study at a tertiary children's hospital.

Purpose: To explore the influence of postoperative Hirschsprung-associated enterocolitis (post-HAEC) on long-term outcomes and to identify risk factors of post-HAEC.

Methods: The medical records of 304 eligible patients diagnosed with Hirschsprung's disease (HSCR) were reviewed. We analyzed the clinical characteristics of post-HAEC and its influence on long-term outcomes.

Pd(II)-catalyzed cascade annulation of -substituted anilines with CO, NHOAc and aldehydes to 1-substituted 2,3-dihydroquinazolin-4(1)-ones.

A facile and direct approach to 1-substituted 2,3-dihydroquinazolin-4(1)-ones has been developed Pd(II)-catalyzed one-pot cascade annulation of -substituted anilines with CO, NHOAc and aldehydes, and it features an intrinsic directing strategy, cheap and easily obtainable raw materials, low cost, high step economy and efficiency, broad substrate scope and good product diversity. This protocol has been successfully applied to the synthesis of glycozolone A and gram-level experiments. Based on the control experiments and the literature, the reaction mechanism was proposed.

Genome assemblies of 11 bamboo species highlight diversification induced by dynamic subgenome dominance.

Polyploidy (genome duplication) is a pivotal force in evolution. However, the interactions between parental genomes in a polyploid nucleus, frequently involving subgenome dominance, are poorly understood. Here we showcase analyses of a bamboo system (Poaceae: Bambusoideae) comprising a series of lineages from diploid (herbaceous) to tetraploid and hexaploid (woody), with 11 chromosome-level de novo genome assemblies and 476 transcriptome samples.