STX2 drives colorectal cancer proliferation via upregulation of EXOSC4.

Aims: To explore the biological function and mechanism of Syntaxin2 (STX2) in Colorectal cancer (CRC) proliferation.

Main Methods: A series of gain- and loss-of-function analysis were conducted the to explore the biological function of STX2 in CRC proliferation in vivo and in vitro. Western blot, Co-immunoprecipitation (Co-IP) and the functional analyses were taken to analyze the regulative role of STX2 on Exosome Complex 4 (EXOSC4) in CRC proliferation; Immunohistochemistry (IHC) and Real-time quantitative polymerase chain reaction (qPCR) were used to further verify the relationship between the expression of STX2 and EXOSC4 in human CRC samples.

targeting by miR-152-3p in Paclitaxel-resistant Breast Cancer.

Paclitaxel plays a pivotal role in the chemotherapy of breast cancer, but resistance to this drug is an important obstacle in the treatment. It is reported that microRNA-152-3p (miR-152-3p) is involved in tamoxifen resistance in breast cancer, but whether it is involved in paclitaxel resistance in breast cancer remains unknown. We examined the expression of miR-152-3p in breast cancer tissues and cells by qRT-PCR.

KISS-1 inhibits the proliferation and invasion of gastric carcinoma cells.

Aim: To investigate the function of the KISS-1 gene in gastric carcinoma cells and to explore its potential mechanism.

Methods: A KISS-1 eukaryotic expression vector was constructed and transfected into BGC-823 cells. Resistant clones were obtained through G418 selection.

[Role of nuclear factor-kappaB in apoptosis pathway of HUVEC].

Aim: To investigate the role of nuclear factor-kappaB in apoptosis pathway of HUVEC.

Methods: The cell lines of HUVEC cultured in vitro were divided into three groups: normal control group, Ang II group, and Gliotoxin group. We investigated the effects of Ang II (0.