Publications by authors named "Guo Xuefang"
Levofloxacin-loaded silicone contact lenses materials for ocular drug delivery.
J Biomater Appl
December 2024
Silicone contact lenses (SCL), as an emerging ocular drug delivery system, achieve controlled drug release. However, the existing drug loading methods have limitations such as low drug uptake, complicated operation process, poor welling rate and transmittance of the lens after drug loading. In this study, an effective microemulsion soaking method was proposed to increase the drug-loading capacity of silicone contact lenses.
View Article and Find Full Text PDFRibose-5-phosphate (R5P) is a precursor for nucleic acid biogenesis; however, the importance and homeostasis of R5P in the intracellular parasite Toxoplasma gondii remain enigmatic. Here, we show that the cytoplasmic sedoheptulose-1,7-bisphosphatase (SBPase) is dispensable. Still, its co-deletion with transaldolase (TAL) impairs the double mutant's growth and increases C-glucose-derived flux into pentose sugars via the transketolase (TKT) enzyme.
View Article and Find Full Text PDFBackground And Aims: This meta-analysis aimed to evaluate the short-term and long-term efficacy of radiofrequency ablation (RFA) and explore the role of diagnostic genicular nerve blocks in predicting treatment outcomes.
Methods: A comprehensive literature search was conducted, and nine randomized controlled trials involving 714 participants were included in the analysis. Data extraction, risk of bias assessment, and subgroup analyses were performed.
Objective: To evaluate the impact of prevention strategies on the quality of life in patients with osteoarthritis (OA) through a comprehensive analysis of randomized controlled trials (RCTs).
Methods: A systematic search was conducted in multiple electronic databases, including Cochrane Library, PubMed, Embase, and ClinicalTrials.gov, up to June 10th, 2023.
This systematic review and meta-analysis aimed to evaluate the effectiveness of COVID-19 vaccines among children and adolescents against SARS-CoV-2 variants. We searched PubMed, Embase, Web of Science, the Cochrane Library, and ClinicalTrials.gov for studies published on or before June 20, 2023.
View Article and Find Full Text PDFBacteria-driven cancer therapy: Exploring advancements and challenges.
Crit Rev Oncol Hematol
November 2023
Article Synopsis
- Cancer is a major health challenge characterized by the uncontrolled growth of cells, making traditional treatments like chemotherapy and radiotherapy often ineffective and harmful to healthy tissues.
- Immunotherapy has shown promise in reducing cancer, particularly through understanding how immune responses can kill cancer cells.
- Bacteria-based cancer immunotherapy is gaining attention for its powerful ability to enhance anti-tumor immune responses and can be used alone or in conjunction with other therapies for better clinical outcomes.
Toxoplasma gondii is a ubiquitous pathogen that infects all warm-blooded animals, including humans, causing substantial socioeconomic and healthcare burdens. However, there is no ideal vaccine for toxoplasmosis. As metabolism is important in the growth and virulence of Toxoplasma, some key pathways are promising antiparasitic targets.
View Article and Find Full Text PDFArticle Synopsis
- Metabolic pathways, especially the pentose phosphate pathway (PPP), are crucial for the growth and virulence of parasites like Toxoplasma gondii, making them potential targets for antiparasitic treatments.
- This study confirms that T. gondii has a functional PPP in its tachyzoite stage and investigates the role of several PPP enzymes by creating mutants that disrupt these enzymes.
- Key findings reveal that while many PPP enzymes are not essential for the parasite's in vitro or in vivo survival, TgG6PDH2 is important for oxidative stress defense, and Tg6PGDH2 and ribulose-5-phosphate isomerase are critical for efficient growth and metabolic function.
Background: Toxoplasma gondii infects almost all warm-blooded animals, and cats play a crucial role in the epidemiology of T. gondii as the definitive host. Despite sporadic reports on the seroprevalence of T.
View Article and Find Full Text PDFThe recombinant protein of Latcripin-4 regulator of chromosome condensation 1 (RCC1) and ankyrin (ANK) domains were expressed and the antitumor activity of Latcripin-4 on HepG2 cells was studied. First, the Latcripin-4 transcript was selected from the medicinal mushroom Lentinus edodes C91-3 transcriptome by bioinformatics. Then the full-length gene of Latcripin-4 was isolated with 3'-full rapid amplification of cDNA ends (RACE) and 5'-full RACE methods according to the transcriptome.
View Article and Find Full Text PDFEffectiveness of omega-3 polyunsaturated fatty acids against microbial pathogens.
J Zhejiang Univ Sci B
October 2018
Article Synopsis
- * The rise of antimicrobial resistance has made traditional treatments less effective, leading researchers to explore dietary compounds, particularly omega-3 fatty acids, for their potential antimicrobial properties.
- * This review emphasizes understanding the effectiveness, mechanisms, and safety of omega-3 fatty acids like linolenic acid in treating infections caused by harmful microbes, as their therapeutic potential hasn't been fully recognized yet.
Article Synopsis
- Inclusion bodies (IBs) in bacteria pose challenges for protein expression, making it hard to recover biochemically active proteins.
- Traditional methods for reducing IBs and solubilizing proteins have limited effectiveness, especially with harsh IBs.
- The new strategy introduced combines mild solubilization techniques with specific conditions (low sarkosyl concentration, slow freezing, and fast thaw) to improve protein solubility and activity recovery significantly compared to older methods.
Article Synopsis
- Pseudomonas aeruginosa is a common Gram-negative pathogen that can cause serious hospital infections, and tobramycin is used to treat lung infections caused by this bacterium.
- The bacteria use quorum sensing to form biofilms, leading to antibiotic resistance, including resistance to tobramycin, necessitating the search for alternative treatments.
- Linolenic acid (LNA) has shown promise in reducing biofilm formation, enhancing the effectiveness of tobramycin, and potentially lowering the dosage required, which could minimize side effects.