Comparison of different methods of nasogastric tube insertion in anesthetized and intubated patients: A meta-analysis.

Background: Several techniques of nasogastric tube (NGT) insertion have been described in the literature with different success rates.

Aim: To systematically search the literature and conduct a meta-analysis comparing the success rates, insertion time and complications associated with different techniques of NGT insertion in anesthetized and intubated patients.

Methods: An electronic search of the PubMed, Scopus, CENTRAL (Cochrane Central Register of Controlled Trials), and Google Scholar databases were performed up to October 31, 2019.

[Molecular epidemiological characteristics of HIV-1 strains isolated from newly diagnosed MSM subjects (2006-2010) in Beijing, China].

This study aims to analyze the molecular epidemiological characteristics of HIV-1 strains prevailing among men who have sex with men (MSM) in Beijing, China. The pol gene fragments from 250 newly diagnosed HIV-1-infected MSM individuals during 2006-2010 in Beijing were amplified by RT-nested PCR, sequenced, and phylogenetically analyzed. HIV-1 pol gene from 189 individuals were amplified and analyzed; 81 (42.

[Analyses on antigen epitopes and drug resistance mutations of HIV-1 gag and pol genes].

To study the CTL antigen epitopes and drug resistance mutations of HIV-1 gag and pol genes through analyzing gag and pol gene sequences. The HIV-1 gag and pol gene fragments were amplified using nested polymerase chain reaction. A total of 23 PCR sequences, 449 cloned gag sequences and 402 cloned pol sequences were obtained.

[Genotyping and variability of HIV-1 in 26 cases of paid blood donors].

Objective: To analyze the genome mutations of HIV-1 gag, pol and env genes from HIV-infected paid blood donors in rural central China.

Methods: DNA was extracted from peripheral blood mononuclear cells, gag (p17-p24), pol (PR-RT), env (C2-V5) genes were amplified by nested polymerase chain reaction (PCR), purified products were sequenced, and sequence data was analyzed by MEGA5.0 soft wares.

[Variation of the V3 loop tip motifs and primary drug resistance of HIV-1 strains].

Objective: To study the characteristics of the variation of the V3 loop tip motifs and the drug resistance in the primary treatment patients.

Methods: The partial region of the HIV-1 env and pol gene in 51 samples was amplified by nested polymerase chain reaction (PCR) ,purified products were cloned into the vectors, the obtained were analyzed by MEGA soft wares.

Results: The V3 loop tip motifs had four types in our study (GPGR, GPGQ, GPGK, GQGR); the study on the drug resistance in primary treatment patients, showed that there were not major resistance associated with PI, and the resistance were minor mutations in protease gene.

[Expression and purification of HIV-1 subtype C Gp120, and its antibodies preparation].

Objective: To prepare HIV-1 subtype C Gp120 protein and to produce its polyclonal antibodies.

Methods: A C-terminal fragment of gp120 gene was amplified by PCR from a plasmid expressing full-length HIV-1 subtype C gp160 gene. The length of the subtype C gp120 fragment was 612 nt and it encodes 204 amino acid residues.

[Study of Gag gene antigen epitypes variation and the quasispecies group characteristics in Henan area HIV-1 strains].

Objective: To study the characteristics of the variation of antigen epitopes and quasispecies group in the HIV-1 viruses in Henan province in China.

Methods: The region of the p17-p24 of the HIV-1 gag gene was amplified by nested polymerse chain reaction (PCR), purified products were cloned into the vector, the obtained were analyzed by MEGA soft wares.

Results: B' subtype strains were predominant in Henan province, the mutations in antigen epitypes of the p17 region of the gag gene focus on E62g (55.

Biological impact of hepatitis B virus X-hepatitis C virus core fusion gene on human hepatocytes.

Aim: To investigate the biological impact of hepatitis B virus X- hepatitis C virus core (HBV X-HCV C) fusion gene on hepatoma cells.

Methods: The recombinant adenoviruses Ad-XC, Ad-X and Ad-C expressing HBV X-HCV C fusion gene, HBV X gene and HCV C gene were constructed, respectively. Hepatoma cells were infected with different recombinant adenoviruses.

[Vasoactive intestinal peptide attenuates apoptosis and iNOS protein expression induced by cerebral ischemia in the rat].

Objective: To explore the neuroprotective action of vasoactive intestinal peptide (VIP) on ischemia and reperfusion in the rat.

Methods: VIP was given via intracerebroventriclar injection after a 2 hour transient middle cerebral artery occlusion using filament model. The infarct volume was investigated with TTC stain.

[Effect of puerarin on PC12 cells apoptosis induced by Abeta25-35 in vitro].

Objective: To establish a nerve cell injury model by incubating PC12 cell line in the presence of Abeta25-35 to study the effect of puerarin on apoptosis of nerve cells.

Methods: PC12 cells were incubated with Abeta25-35 and puerarin. Cell viability was detected by MTT.

[Construction and expression of fusion gene encoding Hyper-IL-6 in mammalian cells].

Aim: To construct and express fusion gene encoding Hyper-IL-6 in mammalian cells.

Methods: Using geneSOEing method, human sIL-6R cDNA was fused with IL-6 cDNA by a linker rich in glycine through PCR technique and cloned into pIRES(2)-EGFP. The constructed plasmid was transfected into mammalian cells.

[Immune potency of recombinant adeno-associated virus combined with recombinant adenovirus vaccine containing HIV-1 gp120].

Objective: To study the immune effect of recombinant adeno-associated virus (rAAV) combined with recombinant adenovirus (rAdV) vaccine in BALB/c mice.

Methods: The codon-modified HIV-1 gp120 gene was inserted into plasmid of adeno-associated virus and adenovirus vector separately. Then the rAAV and rAdV vaccines were constructed.

[Construction and immune potency of recombinant adenovirus containing codon-modified HIV-1 gp120].

Background: To construct replication-deficient recombinant adenovirus expressing wild and codon-modified HIV-1 gp120.

Methods: The viral codons were changed to the codon usage of highly expressed mammal gene, the resulting modified gp120 gene was synthesized. The wild and modified gp120 genes were cloned into shuttle vector pShuttle-CMV respectively, and then the constructed plasmids containing gp120 gene was cotransformed with the backbone vector pADeasy-1 into E.

[Construction and analysis of activity of an HIV-1/bovine immunodeficiency virus chimeric clone cDNA].

Objective: Chimeric human/bovine immunodeficiency virus (HBIV) cDNA was constructed by replacing HIV tat and LTR with bovine immunodeficiency virus (BIV) tat and LTR to study the activity of BIV tat and LTR in the chimerae.

Methods: The target fragments of BIV tat, LTR and HIV gag, pol, env were respectively amplified by using PCR and sequentially inserted into pBluescript SK(+) vector. The chimeric clone was transfected into human MT4 cells.