A novel triple signal amplification platform of peroxide test strip for sensitive detection of adenosine triphosphate.

Peroxide test strip (PTS) has been widely used for the point-of-care testing (POCT), but its poor sensitivity is a big obstacle for analyzing trace target. Herein, a triple signal amplifying platform integrating the liposome enrichment, the hybridization chain reaction (HCR) circuit, and the image analysis method was constructed to enhance the visual readout of PTS for the sensitive detection of adenosine triphosphate (ATP), an important biomarker of food spoilage. In the presence of ATP, the HCR amplifier was firstly initiated on the surface of magnetic beads (MBs), inducing a large number of glucose oxidase (GOD) imbedded liposomes to be attached.

[Effects of fluid resuscitation programs on the levels of inflammatory mediators during burn shock stage].

Objective: To explore the effects of different methods of fluid resuscitation on the levels of inflammatory mediators during burn shock stage.

Methods: Twenty-four miniature swine were numbered from 1 to 24 and randomly divided by EXCEL 2007 into 4 groups of succinylated gelatin, hydroxyethyl starch, Parkland and allogeneic plasma (n = 6 each). Severe burn shock model was established.

[Effects of resuscitation with different kinds of colloids on pulmonary edema in swine in shock stage of severe burn injury].

Objective: To observe and compare the effects of natural colloid and artificial colloid on pulmonary edema of swine during shock stage of severe burn injury.

Methods: Twelve Guangxi Bama miniature swine were inflicted with 40% TBSA full-thickness burn on the back, and then they were divided into natural colloid group (N) and artificial colloid group (A) according to the random number table, with six swine in each group. At post injury hour (PIH) 2, fluid resuscitation was begun.

Effect of artificial colloids on blood coagulation during shock stage of severe burn injury.

Background: There are controversies about the use of artificial colloids. This research was aimed to determine the effect of various artificial colloids on blood coagulation in the shock stage of severe burn injury.

Methods: Totally, 18 female Ba-Ma mini-pigs were subjected to a 40% total body surface third-degree flame burn under anesthesia.

[Shock modeling of large area third-degree burn in miniature swine].

Objective: To report a novel method of establishing a stable shock model of swine.

Methods: A total of 12 Guangxi BA-MA female miniature swines (aged 4-6 months) into A and B groups according to a random number table. Total body surface area (TBSA) was calculated according to the Meeb-Rubner formula.

[Comparison among several foam dressings in the properties of water-absorption, water-locking and air permeability].

Objective: To compare the properties of water-absorption, water-locking, and air permeability among several foam dressings, and to provide references for clinician in choosing dressings for different types of wounds.

Methods: The comparison was made among Allevyn foam dressing, Mepilex foam dressing, and Biatain foam dressing that were commonly used in clinic. NaCl and CaCl2·H2O respectively in the weight of 8.

[Tangential excision of deep partial thickness burn wound during an early stage without tourniquet].

Objective: To explore the clinical efficacy of tangential excision of deep partial thickness burn wound without tourniquet during an early stage.

Methods: A total of 32 patients with a large area of deep partial thickness burn wounds were operated within 24 h post-burn. The lamellar tangential excision was on the basis of stable index of resuscitation from shock without tourniquet.

[Evaluation of the clinical curative effect of applying vacuum sealing drainage therapy in treating deep partial-thickness burn wound at the initial stage].

Objective: To evaluate the clinical curative effect of applying vacuum sealing drainage (VSD) therapy in treating deep partial-thickness burn wound at the initial stage prospectively, and to provide the basis for its clinical application.

Methods: Twenty-two patients with about 10% TBSA burn of the lower limbs, and in which partial-thickness wound exceeded 1% TBSA in each limb, were admitted to our hospital within 3 hours after burn from May 2009 to March 2010. Wounds in each patient were divided into VSD treatment group (treated with VSD therapy) and control group (treated with 10 g/L silver sulfadiazine cream) based on the principles of symmetry of location, identical deepness, and similarity in size etc.

[The relationship between rhGH and blood sugar on different ages of severe degree burned patients].

Objective: Probe the effects of rhGH on severe degree burned patients' blood sugar in different age of years.

Methods: Elected 210 patients hospitalized in the Third Affiliated Hospital of Wenzhou Medical College from January 2005 to December 2008, who were burned in 48 h, older than 18 years, ever had no diabetes and tumor history and placidly pull through shock stage. Among the patients there were 132 males and 78 females.

[Effect of different dressing materials in various combinations on wound microenvironment of donor site in burn patients].

Objective: To investigate the effect of dressing materials in various combinations on burn wound microenvironment and healing condition.

Methods: Two hundred donor sites with wounds of 0.3 mm in depth in 186 burn patients, who needed skin grafting and admitted to our ward were enrolled in study, and they were divided into A (with dressing composed of alginate + cotton pad for donor area), B (with dressing composed of vaseline gauze + cotton pad for donor area), C (with dressing composed of alginate + foam dressing for donor area), D (with dressing composed of vaseline gauze + foam dressing for donor area) groups according to random table method.

Randomized controlled trial of the absorbency of four dressings and their effects on the evaporation of burn wounds.

Background: Wound dressings are divided into traditional and new types. The new dressings are thought to accelerate wound healing. The purpose of this study was to supplement the scanty data on the absorbency of the new dressings and their effects on evaporation from the burn surface.