Developmental validation of the STRSeqTyper122 kit for massively parallel sequencing of forensic STRs.

Massively parallel sequencing allows for integrated genotyping of different types of forensic markers, which reduces DNA consumption, simplifies experimental processes, and provides additional sequence-based genetic information. The STRseqTyper122 kit genotypes 63 autosomal STRs, 16 X-STRs, 42 Y-STRs, and the Amelogenin locus. Amplicon sizes of 117 loci were below 300 bp.

Sequence polymorphisms of forensic Y-STRs revealed by a 68-plex in-house massively parallel sequencing panel.

Sequence polymorphisms of Y chromosome short tandem repeat (Y-STR) markers can be unveiled using next generation sequencing (NGS). Compared to capillary electrophoresis, NGS has the advantage of distinguishing between some alleles of the same length. Here, a 68-plex in-house panel covering 67 Y-STR loci and the sex determinant Amelogenin locus, was developed.

[Epidemiological characteristics of malaria prevalence in Danyang City from 2004 to 2015].

Objective: To analyze the epidemiological characteristics of malaria prevalence in Danyang City from 2004 to 2015, so as to provide the evidence for formulating the strategy of prevention and control of malaria.

Methods: The data of malaria serum tests, the reported malaria cases from the Internet Reporting System, and the epidemiological case survey from 2004 to 2015 as well as the mosquito monitoring data from 2008 to 2015 were collected and analyzed.

Results: From 2004 to 2015, 58 malaria cases were reported in Danyang City, with an average annual incidence rate of 0.