Publications by authors named "Guo Hong Li"

Purpose: This study aims to compare the difference in serum high-density lipoprotein cholesterol (HDL-C) levels between children with epilepsy and healthy children and to assess its potential influencing factors.

Methods: For comparison, we retrospectively collected data on 1,002 children with epilepsy who visited the Department of Neurology at the Children's Hospital of Nanjing Medical University. Additionally, we included 127 healthy children who underwent routine health examinations at our hospital's Health Examination Center.

View Article and Find Full Text PDF

A-kinase anchoring protein 79/150 (AKAP79/150) is a crucial scaffolding protein that positions various proteins at specific synaptic sites to modulate excitatory synaptic intensity. As our understanding of AKAP79/150's biology deepens, along with its significant role in the pathophysiology of various human disorders, there is growing evidence that reveals new opportunities for therapeutic interventions. In this review, we examine the fundamental structure and primary functions of AKAP79/150, emphasizing its pathophysiological mechanisms in different nervous system disorders, particularly inflammatory pain, epilepsy, depression, and Alzheimer's disease.

View Article and Find Full Text PDF

Root-knot nematodes (RKNs) are pathogens that endanger a wide range of crops and cause serious global agricultural losses. In this study, we investigated metabolites of the endoparasitic fungus YMF1.01751, with the expectation of discovering valuable biocontrol compounds.

View Article and Find Full Text PDF

The maintenance of genomic integrity in rapidly proliferating cells is a substantial challenge during embryonic development. Although numerous cell-intrinsic mechanisms have been revealed, little is known about genome-protective effects and influences of developmental tissue microenvironments on tissue-forming cells. Here we show that fetal liver hepatocytes provide protection to haematopoietic stem and progenitor cell (HSPC) genomes.

View Article and Find Full Text PDF

Non-stimulant atomoxetine is recognized in various current clinical guidelines as an important alternative to stimulants for the pharmacological treatment of attention deficit/hyperactivity disorder (ADHD) in children. While its efficacy and tolerability for core symptoms are established, there is considerable inter-individual variability in response and exposure, highlighting the need for personalized dosing. In this review, we evaluated existing studies and summarized comprehensive evidence supporting the clinical implementation of therapeutic drug monitoring (TDM) and personalized dosing of atomoxetine, organized around a series of logically structured questions.

View Article and Find Full Text PDF
Article Synopsis
  • Sirolimus (SRL) is used to treat vascular anomalies in children, and this study aimed to evaluate blood SRL levels, factors affecting these levels, and the treatment's effectiveness and safety.
  • The study analyzed data from 67 children over a period of five years, finding that only 33% initially had desired SRL levels, which improved to 54% by the end, indicating variability in drug concentrations.
  • While 70.3% of participants showed a positive response to SRL, adverse events occurred in 44.8% of cases, although they were mostly mild; further research is needed to establish an appropriate target concentration range for this population.
View Article and Find Full Text PDF
Article Synopsis
  • Sirolimus is a medicine used for children with vascular anomalies, but it can be tricky to figure out the right dose because it acts differently in each child.
  • Researchers studied how sirolimus works in 49 kids and found that body weight is a big factor in how quickly the body clears the medicine.
  • They created a new model to help doctors decide the best doses for children based on their weight and suggested specific dosing plans for twice-daily and once-daily use.
View Article and Find Full Text PDF

Nematode-trapping (NT) fungi are a major resource for controlling parasitic nematodes. , as a typical NT fungus, can capture nematodes by producing three-dimensional nets. The APSES transcription factor plays a vital role in fungal growth and the pathogenicity of pathogens.

View Article and Find Full Text PDF

Background: The widespread clinical use of lacosamide (LCM) has revealed significant individual differences in clinical response, with various reported influencing factors. However, it remains unclear how genetic factors related to the disposition and clinical response of LCM, as well as drug-drug interactions (DDIs), exert their influence on pediatric patients with epilepsy.

Objectives: To evaluate the impact of genetic variations and DDIs on plasma LCM concentrations and clinical response.

View Article and Find Full Text PDF
Article Synopsis
  • The study investigates the use of pharmacogenetics for personalized medication in children's hospitals across Mainland China, aiming to assess its implementation and effectiveness.
  • A survey was conducted at 50 hospitals, with a 76% response rate, revealing limited use of genetic testing; only a few hospitals conducted comprehensive tests, and many lacked proper quality control and training for practitioners.
  • Despite progress in pediatric pharmacogenomics, challenges remain in infrastructure, training, and funding, necessitating collaboration among health administrators, insurance bodies, and hospitals to enhance GTPM practices.
View Article and Find Full Text PDF

Background: Several population pharmacokinetic (PopPK) models of caffeine in preterm infants have been published, but the extrapolation of these models to facilitate model-informed precision dosing (MIPD) in clinical practice is uncertain. This study aimed to comprehensively evaluate their predictive performance using an external, independent dataset.

Methods: Data used for external evaluation were based on an independent cohort of preterm infants.

View Article and Find Full Text PDF

Background: Considerable interindividual variability for the pharmacokinetics of caffeine in preterm infants has been demonstrated, emphasizing the importance of personalized dosing. This study aimed to develop and apply a repository of currently published population pharmacokinetic (PopPK) models of caffeine in preterm infants to facilitate model-informed precision dosing (MIPD).

Research Design And Methods: Literature search was conducted using PubMed, Embase, Scopus, and Web of Science databases.

View Article and Find Full Text PDF
Article Synopsis
  • * The study collected 1411 VPA steady-state trough concentrations from 617 children and used NONMEM software to create the model, which identified key variables affecting VPA pharmacokinetics such as body weight and sex.
  • * The final model suggested a daily dose of 25 mg/kg for syrup and 20 mg/kg for sustained-release tablets to achieve optimal plasma levels, aiming to improve treatment outcomes by minimizing fluctuations in VPA concentrations.
View Article and Find Full Text PDF

Objective: To explore the value of serum D-dimer (D-D), fibrinogen (FIB), platelet (PLT), C-reactive protein (CRP) and tissue plasminogen activator inhibitor (PAI)-1 levels in predicting lower extremity deep vein thrombosis (DVT) after hip joint surgery in the elderly.

Methods: A retrospective analysis was performed on 165 elderly patients with hip joint surgery admitted from February 2020 to May 2022, including 89 males and 76 females, aged from 60 to 75 years old with an average of (66.43±5.

View Article and Find Full Text PDF

Cyclosporine A (CsA) is a widely used immunosuppressive drug with a narrow therapeutic index and large individual differences. Its therapeutic and toxic effects are closely related to blood drug concentrations, requiring routine therapeutic drug monitoring (TDM). The current main methods for TDM of CsA are enzyme multiplied immunoassay technique (EMIT) and liquid chromatography-tandem mass spectrometry (LC-MS/MS).

View Article and Find Full Text PDF
Article Synopsis
  • The study focuses on how biogeographic dispersal has impacted plant diversity in the Himalaya-Hengduan Mountains, specifically examining the alpine clade Gaultheria ser. Trichophyllae.
  • Through phylogenetic analysis, researchers analyzed dispersal patterns, revealing a trend of eastward dispersals within regions and westward dispersals between regions, influenced by climatic changes over time.
  • The findings suggest that the timing of these dispersals, especially during glacial and interglacial periods, plays a crucial role in shaping the current species richness of G. ser. Trichophyllae.
View Article and Find Full Text PDF

Integrating CYP2D6 genotyping and therapeutic drug monitoring (TDM) is crucial for guiding individualized atomoxetine therapy in children with attention-deficit/hyperactivity disorder (ADHD). The aim of this retrospective study was (1) to investigate the link between the efficacy and tolerability of atomoxetine in children with ADHD and plasma atomoxetine concentrations based on their CYP2D6 genotypes; (2) to offer TDM reference range recommendations for atomoxetine based on the CYP2D6 genotypes of children receiving different dosage regimens. This retrospective study covered children and adolescents with ADHD between the ages of 6 and <18, who visited the psychological and behavioral clinic of Children's Hospital of Nanjing Medical University from June 1, 2021, to January 31, 2023.

View Article and Find Full Text PDF

Objective: To evaluate the effectiveness and safety of perampanel (PER) monotherapy (MT) or add-on therapy (AT) in Chinese children with epilepsy, as well as to evaluate the data from routine therapeutic drug monitoring (TDM) of PER for these pediatric patients.

Methods: This retrospective and observational study was carried out on children with epilepsy (n = 340) from 2020 to 2022 at the Children's Hospital of Nanjing Medical University. Outcome measures were the responder rate (50% or greater seizure reduction), long-term efficacy, and tolerability (number and types of adverse events) in MT and AT groups.

View Article and Find Full Text PDF

is a typical nematode-trapping fungus that has garnered the attention of many scholars for its highly effective lethal potential for nematodes. Secondary metabolites play an important role in -nematode interactions, but which metabolites perform which function remains unclear. We report the metabolic functions based on high-quality, chromosome-level genome assembly of wild YMF1.

View Article and Find Full Text PDF

The inefficient treatment using protein-based nanovaccines is largely attributed to their inadequate immunogenicity. Herein, we developed a novel fluoropolymer (PF) ring-opening polymerization and constructed a fluoropolymer-based nanovaccine for tumor immunotherapy. Due to the existence of fluoroalkyl chains, PF not only played a crucial role in tumor antigen delivery but also exhibited a remarkable adjuvant effect in enhancing the immunogenicity of nanovaccines.

View Article and Find Full Text PDF

Objectives: To update traditional "wet" matrices to dried blood spot (DBS) sampling, based on the liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) technique, and develop a method for simultaneous analyzing caffeine and its three primary metabolites (theobromine, paraxanthine, and theophylline), supporting routine therapeutic drug monitoring (TDM) for preterm infants.

Methods: DBS samples were prepared by a two-step quantitative sampling method, i.e.

View Article and Find Full Text PDF
Article Synopsis
  • Arthrobotrys flagrans
  • is a nematode-trapping fungus important for biocontrol, whose genome's LaeA gene plays a significant role in growth, development, and metabolism.
  • - Knocking out the LaeA gene resulted in slower growth, lack of chlamydospores, disrupted energy storage, and decreased ability to trap nematodes.
  • - The study reveals LaeA's vital functions in mycelial growth, secondary metabolism, and energy regulation in nematode-trapping fungi, highlighting a gap in research around its role compared to other types of fungi.
View Article and Find Full Text PDF

Valproic acid (VPA) is a well-documented contributor to liver injury, which is likely caused by the formation of its toxic metabolites. Monitoring VPA and its metabolites is very meaningful for the pharmacovigilance, but the availability of a powerful assay is a prerequisite. In this study, for the first time, a sensitive and specific LC-MS/MS method was developed and validated to simultaneously quantify the concentrations of VPA and its six pestering isomer metabolites (3-OH-VPA, 4-OH-VPA, 5-OH-VPA, 2-PGA, VPA-G, and 2-ene-VPA) in human plasma, using 5-OH-VPA-d7 and VPA-d6 as the internal standards (ISs).

View Article and Find Full Text PDF

Vigabatrin is one of the second-generation anti-seizure medications (ASMs) designated orphan drugs by the FDA for monotherapy for pediatric patients with infantile spasms from 1 month to 2 years of age. Vigabatrin is also indicated as the adjunctive therapy for adults and pediatric patients 10 years of age and older with refractory complex partial seizures. Ideally, the vigabatrin treatment entails achieving complete seizure freedom without significant adverse effects, and the therapeutic drug monitoring (TDM) will make a significant contribution to this aim, which provides a pragmatic approach to such epilepsy care in that the dose tailoring can be undertaken for uncontrollable seizures and in cases of clinical toxicity guided by the drug concentrations.

View Article and Find Full Text PDF