YBX1 Modulates Intimal Hyperplasia by Regulating Expression and Alternative Splicing of Cell Cycle Associated Genes in RASMCs.

YBX1, a DNA-/RNA-binding protein, is implicated in various diseases, yet its role in intimal hyperplasia (IH) remains unclear. This study investigates YBX1's function in rat aortic smooth muscle cells (RASMCs) through knockdown experiments. Results show that YBX1 knockdown reduces cell proliferation and migration while inducing apoptosis.

A novel technique for microwave ablation of malignant pulmonary nodules: electromagnetic navigation bronchoscopy with real-time digital subtraction angiography and computed tomography imaging guidance.

Objectives: Electromagnetic navigation bronchoscopy (ENB) has been used to guide the diagnosis and treatment of pulmonary nodules. However, its combined application with real-time digital subtraction angiography (DSA), computed tomography (CT) and microwave ablation (MWA) for malignant pulmonary nodules has not been documented. This study aimed to evaluate the feasibility and safety of a novel technique that integrates ENB with real-time two-dimensional (2D) and three-dimensional (3D) imaging guidance for MWA of malignant pulmonary nodules.

Study on mechanism behind browning of Antarctic krill upon hot air drying and its control.

Cooked Antarctic krill (AK) was sprayed with antioxidants, followed by hot air drying (HAD) to produce AK meal (AKM). The antioxidants could alleviate color darkening, reduce oxidation of lipids and proteins, among which tea polyphenol palmitate (TPP) + antioxidant of bamboo leaf (AOB) exhibited the best effect on improving overall quality of AKM. The color indicator (L*) was negatively correlated with hydrophilic pyrrole, hydrophobic pyrrole and lipid oxidation indexes, suggesting that Maillard-like reaction and lipid oxidation contributed to the color darkening.

Changes in the physicochemical properties and structural characteristics of rice bran polysaccharides extracted by specific enzyme cocktail and ultrasound.

To maximize insoluble rice bran fiber, rice bran polysaccharides were extracted from it using ultrasound and specific enzyme-assisted methods. Additionally, the specific enzyme cocktail was also produced by Schizophyllum commune cultivated on insoluble rice bran fiber. Based on response surface methodology, the optimal conditions of specific enzyme-assisted extraction were: temperature 42.

Clinical Application of 68Ga-Pentixafor PET/CT for the Detection of Thymoma: A Pilot Study.

Purpose: This study was designed to evaluate 68Ga-pentixafor, which targets C-X-C chemokine receptor 4 (CXCR4), in the noninvasive diagnosis of thymomas.

Patients And Methods: With institutional review board approval and signed informed consent, 32 patients with thymic masses were enrolled in this study, and all patients underwent 68Ga-pentixafor PET/CT scans.

Results: Among the 28 patients included in the analysis, 13 patients were diagnosed with thymomas, 9 patients with cysts, and 6 patients with other anterior mediastinal masses.

Perioperative Treatment in EGFR-Mutant Early-Stage Non-Small Cell Lung Cancer: Current Evidence and Future Perspectives.

Adjuvant osimertinib administered over a 3-year period in patients diagnosed with stage IB-IIIA non-small cell lung cancer (NSCLC) and epidermal growth factor receptor (EGFR) mutations has not only shown improvement in event-free survival but also demonstrated a prolonged overall survival (OS), leading to its approval as a standard treatment in this context. Meanwhile, no targeted studies have been conducted on the efficacy of adjuvant immune checkpoint inhibitors in these patients. Although studies such as IMPOWER-010 and KEYNOTE-091 have included a small number of patients with positive driver genes, no definitive conclusions regarding the OS benefit have been established.

Systemic Immune-Inflammation Index as a Predictor of Survival in Non-Small Cell Lung Cancer Patients Undergoing Immune Checkpoint Inhibition: A Systematic Review and Meta-Analysis.

Background: This meta-analysis aims to evaluate the association between pretreatment systemic immune-inflammation index (SII) levels and progression-free survival (PFS) and overall survival (OS) in NSCLC patients receiving immune checkpoint inhibitors (ICIs).

Methods: A systematic search was conducted across PubMed, Embase, and Web of Science. Hazard ratios (HRs) with 95% confidence intervals (CIs) for PFS and OS were extracted or calculated.

Effects of hydraulic circulation induced by gravel lenses of varying sizes on nitrobenzene removal in heterogeneous aquifers during air sparging remediation.

Air sparging (AS) technology is widely used in groundwater remediation of organic contaminants. In this study, using a two-dimensional physical model, the research explores how varying sizes of gravel lenses affect airflow distribution, hydraulic circulation, and contaminant removal efficiency. The presence of gravel lenses was found to significantly enhance hydraulic circulation, thereby improving the removal of nitrobenzene.

Transcriptomic and biochemical insights into key gene networks driving bulbil development of Pinellia ternata (Thunb.) Breit.

In this study, we explored the developmental characteristics of Pinellia ternate bulbils as well as the key gene networks driving the development of bulbils. Based on physiological and biochemical reactions as well as transcriptome technology, this study determined the content of endogenous metabolites and related enzyme activities during the five growth stages of the bulbils, obtained the transcriptome information of all samples. The results showed that the contents of sucrose and starch increased significantly in the ZY_2 and ZY_4 stages, and the changes in the activities of SPS, SuSy, and SS were basically consistent with the changing characteristics of sucrose and starch content.

Quantifying multilabeled brain cells in the whole prefrontal cortex reveals reduced inhibitory and a subtype of excitatory neuronal marker expression in serotonin transporter knockout rats.

The prefrontal cortex regulates emotions and is influenced by serotonin. Rodents lacking the serotonin transporter (5-HTT) show increased anxiety and changes in excitatory and inhibitory cell markers in the prefrontal cortex. However, these observations are constrained by limitations in brain representation and cell segmentation, as standard immunohistochemistry is inadequate to consider volume variations in regions of interest.

The regenerative wound healing effects and molecular mechanism of Isaria cicadae Miquel rice fermentation extract.

Human skin wounds primarily heal through reparative wound healing without pilosebaceous units or other appendages, rather than regenerative wound healing. Hair follicle (HF) regeneration is a significant challenge for skin wound healing. The effects and underlying mechanisms of Isaria cicadae Miquel rice fermentation extract (IMFRE) remain unclear, although it has anti-inflammatory, antioxidant, and reparative effects on oxidative damage in keratinocytes.

Association of household carbon consumption with cardiovascular diseases: A multicohort study.

The household sector is a major contributor to CO2 emissions. Despite growing concerns about the sector's impacts on public health, an exploration of its association with cardiovascular disease (CVD) using a longitudinal dataset across multiple countries remains scarce. We conducted a multicohort study across three countries (China, Japan, and Australia) with 36 824 participants aged 18-64, free of diagnosed CVD.

Cell-free epigenomes enhanced fragmentomics-based model for early detection of lung cancer.

Background: Lung cancer is a leading cause of cancer mortality, highlighting the need for innovative non-invasive early detection methods. Although cell-free DNA (cfDNA) analysis shows promise, its sensitivity in early-stage lung cancer patients remains a challenge. This study aimed to integrate insights from epigenetic modifications and fragmentomic features of cfDNA using machine learning to develop a more accurate lung cancer detection model.

Mechanisms of epigallocatechin-3-gallate-loaded metal-organic framework in preventing oxidative degradation of shrimp (Litopenaeus vannamei) surimi gel.

This work aimed to elucidate the deterioration mechanisms of shrimp surimi gels during refrigerated storage, and the regulatory mechanisms of epigallocatechin-3-gallate loaded cyclodextrin-based metal-organic framework (EGCG@CD-MOF) as a model antioxidant. Labele-free proteomics provided a quantitative analysis of the differential proteomic signatures of degraded proteins. Structural proteins, like myosin, paramyosin, titin, laminin, and α-actinin, along with calcium regulatory proteins, like calcineurin and sarcoplasmic calcium-binding protein were found to be highly susceptible to oxidative degradation during refrigeration.

Irisin reshapes bone metabolic homeostasis to delay age-related osteoporosis by regulating the multipotent differentiation of BMSCs via Wnt pathway.

Introduction: Bone aging is linked to changes in the lineage differentiation of bone marrow stem cells (BMSCs), which show a heightened tendency to differentiate into adipocytes instead of osteoblasts. The therapeutic potential of irisin in addressing age-related diseases has garnered significant attention. More significantly, irisin has the capacity to enhance bone mass recovery and sustain overall bone health.

The PurR family transcriptional regulator promotes butenyl-spinosyn production in Saccharopolyspora pogona.

Butenyl-spinosyn, derived from Saccharopolyspora pogona, is a broad-spectrum and effective bioinsecticide. However, the regulatory mechanism affecting butenyl-spinosyn synthesis has not been fully elucidated, which hindered the improvement of production. Here, a high-production strain S.

Immobilization of ammonia-oxidizing bacteria using mycelial pellets: Preparation, characteristics, and application for nitritation.

Ammonia-oxidizing bacteria (AOB) sourced from an aerobic granular sludge (AGS) process were rapidly enriched by progressively increasing ammonia nitrogen (NH-N) loads, achieving a Nitrosomonas abundance of 20.7 % and a nitrite accumulation rate exceeding 80 %. Mycelial pellets formed by Cladosporium, isolated from the same AGS system, provided a porous surface structure for the immobilization of the enriched AOB, creating mycelial pellet/AOB composites.

Geochemical speciation and activation risks of Cd, Ni, and Zn in soils with naturally high background in karst regions of southwestern China.

Agricultural soils in karst regions present a remarkable paradox where high geochemical background levels of heavy metals correspond with unexpectedly low crop uptake, challenging traditional risk assessment frameworks and limiting agricultural development. To decode this paradox, we investigated the geochemical speciation of cadmium (Cd), nickel (Ni), and zinc (Zn) in soil-rice systems in southwestern China, which collectively constitute the world's largest continuous karst region and represent diverse soil weathering stages. We employed three chemical extraction methods that revealed reactive pools ranking as Cd (58.

Cross-kingdom regulation of plant microRNAs: potential application in crop improvement and human disease therapeutics.

Plant microRNAs (miRNAs) are small non-coding RNA molecules that usually negatively regulate gene expression at the post-transcriptional level. Recent data reveal that plant miRNAs are not limited to individual plants but can transfer across different species, allowing for communication with the plant, animal, and microbial worlds in a cross-kingdom approach. This review discusses the differences in miRNA biosynthesis between plants and animals and summarizes the current research on the cross-species regulatory effects of plant miRNAs on nearby plants, pathogenic fungi, and insects, which can be applied to crop disease and pest resistance.

Structural remodeling of the brain cortex and functional recovery following hypoglossal-facial neurorrhaphy in patients with facial paralysis.

Objective: Peripheral nerve injury results in functional alterations of the corresponding active brain areas, which are closely related to functional recovery. Whether such functional plasticity induces relative anatomical structural changes remains to be investigated.

Methods: In this study, we investigated the changes in brain cortical thickness in patients with facial paralysis following neurorrhaphy treatment at different follow-up times.

Localization strategies for deep lung nodule using electromagnetic navigation bronchoscopy and indocyanine green fluorescence: a technical note.

Accurate localization of pulmonary nodules is crucial for successful video-assisted thoracoscopic surgery (VATS) resection. Electromagnetic navigation bronchoscopy (ENB) combined with indocyanine green (ICG) fluorescence has emerged as a promising technique for precise pulmonary nodule marking. This study aims to evaluate the efficacy and safety of four ENB-guided ICG marking techniques: direct lesion marking, superficial marking, resection boundary marking, and margin sphere marking.

Correlations between 14-gene RNA-level assay and clinical and molecular features in resectable non-squamous non-small cell lung cancer: a cross-sectional study.

Background: Non-small cell lung cancer (NSCLC) is the leading cause of cancer-related death worldwide. Accurate risk stratification is essential for optimizing treatment strategies. A 14-gene RNA-level assay of lung cancer, which involves quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis of formalin-fixed paraffin-embedded (FFPE) tissue samples, offers a promising approach.

Cancer situation in China: an analysis based on the global epidemiological data released in 2024.

Background: Cancer remains a major cause of mortality and a significant economic burden in China. Exploring the disparities in cancer patterns and control strategies between China and developed countries may offer valuable insights for policy formulation and enhance cancer management efforts. This study examined the incidence, mortality, and disability-adjusted life year (DALY) burden of cancer in China, and compared these metrics with those observed in the United States (US) and the United Kingdom (UK).

[Advances in the Application of Adjuvant Chemotherapy and Targeted Therapy in Postoperative Patients with Stage I Lung Adenocarcinoma].

Lung cancer is one of the main causes of cancer burden and death in China, with nearly 800,000 newly diagnosed lung cancer patients each year, nearly half of whom are lung adenocarcinoma (LUAD) patients. According to current clinical guidelines, surgery is the main treatment for stage I LUAD patients, but the 5-year overall survival rate of stage I LUAD patients alone is still unsatisfactory, about 73%-90%, indicating that a considerable number of patients require other means to improve survival benefits. Chemotherapy and targeted therapy have achieved great success in the treatment of locally advanced and metastatic LUAD patients, but there is still controversy over whether they can benefit stage I LUAD postoperative patients.