A comparative analysis of tranexamic acid dosing strategies in traumatic major hemorrhage.

Introduction: Tranexamic acid (TXA) is a life-saving treatment for traumatic hemorrhage, but the optimal dosing regimen remains unknown. Different doses and treatment strategies have been proposed, including single bolus, repeated bolus, or bolus plus infusion. The aim of this study was to determine the effect of different TXA dosing strategies on clinical outcomes in bleeding trauma patients.

Double faecal immunochemical testing in patients with symptoms suspicious of colorectal cancer.

Background: Faecal immunochemical test (FIT)-directed pathways based on a single test have been implemented for symptomatic patients. However, with a single test, the sensitivity is 87 per cent at 10 µg haemoglobin (Hb) per g faeces. This aims of this study were to define the diagnostic performance of a single FIT, compared with double FIT in symptomatic populations.

Development of a customised data management system for a COVID-19-adapted colorectal cancer pathway.

Objectives: A customised data management system was required for a rapidly implemented COVID-19-adapted colorectal cancer pathway in order to mitigate the risks of delayed and missed diagnoses during the pandemic. We assessed its performance and robustness.

Methods: A system was developed using Microsoft Excel (2007) to retain the spreadsheets' intuitiveness of direct data entry.

Short-term outcomes of a COVID-adapted triage pathway for colorectal cancer detection.

Aim: The dramatic curtailment of endoscopy and CT colonography capacity during the coronavirus pandemic has adversely impacted timely diagnosis of colorectal cancer (CRC). We describe a rapidly implemented COVID-adapted diagnostic pathway to mitigate risk and maximize cancer diagnosis in patients referred with symptoms of suspected CRC.

Method: The 'COVID-adapted pathway' integrated multiple quantitative faecal immunochemical tests (qFIT) to enrich for significant colorectal disease with judicious use of CT with oral contrast to detect gross pathology.

Topological analyses of the L-fucose-H+ symport protein, FucP, from Escherichia coli.

The transport of L-fucose into Escherichia coli is mediated by the L-fucose-H+ symport protein (FucP). The fucP gene has been sequenced and encodes a hydrophobic protein that contains 438 amino acid residues, with a predicted M(r) of 47,773. The hydropathic profile of FucP indicates 10 to 12 hydrophobic regions that could span the membrane as alpha-helices.

Identification of a novel sugar-H+ symport protein, FucP, for transport of L-fucose into Escherichia coli.

L-Fucose (6-deoxy-L-galactose) is used as sole carbon source by many microorganisms, and its transport into Escherichia coli is mediated by an L-fucose-H+ symport activity. In order to determine the nature of a putative transporter encoded by the E. coli fucP gene and identify its protein product it was cloned downstream of the inducible T7 RNA polymerase and lambda OLPL promoters.

Structural basis for the kinetic differences between flavocytochromes b2 from the yeasts Hansenula anomala and Saccharomyces cerevisiae.

To understand the structural basis for the different catalytic behaviour of the flavocytochromes b2 from Saccharomyces cerevisiae and Hansenula anomala we have cloned and sequenced the gene encoding the latter. We have compared the amino acid sequences of the mature proteins in the context of the known crystal structure of S. cerevisiae flavocytochrome b2.