[Thioredoxin-reductase in fibroblasts of human dermis in the process of aging.].

The aim of this work was to examine the content of thioredoxin-reductase in fibroblasts of human dermis from the development until deep aging (from 20 weeks of pregnancy until 85 years old), and defining of a role of thioredoxin-reductase in age-dependent changes in the number of fibroblasts in the dermis. Thioredoxin-reductase, proliferating cells nuclear antigen (PCNA), marker of fibroblasts vimentin were detected with indirect immunohistochemical technique. Results showed that portion of fibroblasts with positive staining for thioredoxin reductase in the dermis is increased from 20 weeks of pregnancy until 20 years old, is not changed from 21 to 60 years old, and is increased again from 61 to 85 years old.

[Thioredoxin interacting protein in fibroblasts of human dermis in the process of aging.].

The aim of this work was to examine the content of thioredoxin interacting protein in fibroblasts of human dermis from the development until 85 years old, and defining of a role of thioredoxin interacting protein in age-dependent changes in the number of fibroblasts in the dermis. Thioredoxin interacting protein, proliferating cells nuclear antigen (PCNA), marker of fibroblasts vimentin were detected with indirect immunohistochemical technique. Results showed that portion of fibroblasts with positive staining for thioredoxin interacting protein in the dermis is increased from 20 weeks of pregnancy until 60 years old followed by a little decrease in age interval 61-85 years old.

[Thioredoxin in fibroblasts of human dermis in the process of aging.].

The aim of this work was to examine the content of thioredoxin in fibroblasts of human dermis from the development until deep aging (from 20 weeks of pregnancy until 85 years old), and defining of a role of thioredoxin in age-dependent changes in the number of fibroblasts in the dermis. Thioredoxin, proliferating cells nuclear antigen (PCNA), marker of fibroblasts vimentin were detected with indirect immunohistochemical technique. Results showed that portion of fibroblasts with positive staining for thioredoxin in the dermis is increased from 20 weeks of pregnancy until 85 years old.

[Changes in the number of p23-positive fibroblasts in human dermis with aging.].

The aim of this work was to examine the content of p23 in fibroblasts of human dermis from 20 weeks of pregnancy until 85 years old, and defining of a role of p23 in age-dependent changes in the number and proliferation of fibroblasts in the dermis. p23, proliferating cells nuclear antigen (PCNA), fibroblasts marker vimentin were detected with indirect immunohistochemical technique. Results showed that portion of fibroblasts with positive staining for p23 in the dermis is gradually increased from 20 weeks of pregnancy until 85 years old.

[Aryl hydrocarbon receptor interacting protein (AIP) in human dermis during aging.].

The aim of this work was to examine the content of aryl hydrocarbon receptor interacting protein (AIP) in fibroblasts of human dermis from 20 weeks of pregnancy until 85 years old, and defining of a role of AIP in age-dependent changes in the number of fibroblasts in the dermis. AIP, proliferating cells nuclear antigen (PCNA) were detected with indirect immunohistochemical technique. Results showed that a portion of fibroblasts with positive staining for AIP in the dermis is gradually increased from 20 weeks of pregnancy until 85 years old.

[Arylhydrocarbon receptor nuclear translocator (ARNT) in human skin during aging.].

The aim of this work was to examine the content of arylhydrocarbon receptor nuclear translocator (ARNT) in fibroblasts of human dermis from 20 weeks of pregnancy until 85 years old, and defining of a role of ARNT in age-dependent changes in the number of fibroblasts in the dermis. ARNT, proliferating cells nuclear antigen (PCNA) were detected with indirect immunohistochemical technique. Results showed that a portion of fibroblasts with positive staining for ARNT in the dermis is decreased from 20 weeks of pregnancy to 40 years old.

[Heat shock protein 90 (HSP90) in age-dependent changes in the number of fibroblasts in human skin.].

The aim of this work was to examine the content of heat shock protein 90 (HSP90) in fibroblasts of human dermis from the development until deep aging (from 20 weeks of pregnancy until 85 years old), and defining of a role of HSP90 in age-dependent changes in the number of fibroblasts in the dermis. HSP90, proliferating cells nuclear antigen (PCNA) were detected with indirect immunohistochemical technique. Results showed that a portion of fibroblasts with positive staining for HSP90 in the dermis is not changed from 20 weeks of development to 20 years old.

[Mechanosensitive Yes-associated protein in human skin during aging.].

The aim of this work was to examine the content of Yes-associated protein (YAP) in fibroblasts and blood microvessels of human dermis from the development until deep aging (from 20 weeks of pregnancy until 85 years old), and defining of a role of YAP in age-dependent changes in the number of fibroblasts and blood microvessels in the dermis. YAP, proliferating cells nuclear antigen (PCNA), endothelial cells marker CD31 were detected with indirect immunohistochemical technique. Results showed that portion of fibroblasts with positive staining for YAP in the dermis is decreased from 20 weeks of pregnancy to 40 years old.

[Role of mechanosensitive protein Piezo1 in human age-dependent changes in the number of fibroblasts and blood vessels in human skin.].

The aim of this work was to examine the content of Piezo1 in fibroblasts and blood vessels of human dermis from the development until deep aging (from 20 weeks of pregnancy until 85 years old), and defining of a role of Piezo1 in age-dependent changes in the number of fibroblasts and blood vessels in the dermis. Piezo1, proliferating cells nuclear antigen (PCNA), endothelial cells marker CD31 were detected with indirect immunohistochemical technique. Results showed that a portion of fibroblasts with positive staining for Piezo1 in the dermis is decreased from 20 weeks of pregnancy to 40 years old.

[Transforming growth factor-β (TGF-β) in human skin in the process of aging.].

The aim of this work was to examine the content of transforming growth factor-β (TGF-β) in fibroblasts and blood microvessels of human dermis from the development until deep aging (from 20 weeks of pregnancy until 85 years old), and defining of a role of TGF-β in age-dependent changes in the number of fibroblasts and blood microvessels in the dermis. TGF-β, proliferating cells nuclear antigen (PCNA), endothelial cells marker CD 31 were detected with indirect immunohistochemical technique. Results showed that portion of fibroblasts with positive staining for TGF-β in the dermis is increased from 20 weeks of pregnancy until 85 years old.

Number, Proliferative Activity, and Expression of Thyroid Hormone Receptors in Dermal Fibroblasts in Mice with Changed Thyroid Status.

We studied the intensity of age-specific changes in the dermis (number and proliferative activity of fibroblasts) in mice with normal and experimentally changed level of thyroid hormones. Receptors of thyroid hormones, TR-α and TR-β, in mouse dermal fibroblasts were identified by immunohistochemical methods. The relative expression of Thra, Thrb, and Dio2 genes was assessed by real-time PCR analysis.

[The influence of metformin on age-related changes in the number and proliferation of dermal fibroblasts in mice.].

The goal of our work was to examine the effects of metformin on age-related changes in the number and proliferation of dermal fibroblasts in mice. The study of the dermis was carried out at five months' mice that received drinking water with metformin at concentrations 500 mg/l from two months (within 90 days). Five months' mice received drinking water without metformin and they were as a control.

[Mechanosensitive protein of Hippo regulatory pathway - transcription coactivator with PZD-binding motif (TAZ) in human skin during aging.].

The aim of this work was to examine the content of transcription coactivator with PZD-binding motif (TAZ) in fibroblasts and blood vessels of human dermis from the development until deep aging (from 20 weeks of pregnancy until 85 years old), and defining of a role of TAZ in age-dependent changes in the number of fibroblasts and blood vessels in the dermis. TAZ, proliferating cells nuclear antigen (PCNA), endothelial cells marker CD31 were detected with indirect immunohistochemical technique. Results showed that portion of fibroblasts with positive staining for TAZ in the dermis is decreased from 20 weeks of pregnancy to 40 years old.

[Thyroid hormone receptors in human skin during aging.].

This work was aimed to study levels of thyroid hormone receptors in human dermal fibroblasts from the development to deep aging. Skin specimens from human fetuses died antenatally from 20 to 40 weeks of pregnancy, humans died from different causes from birth to 85 years of life were used for the study. Total number of fibroblasts, percent of proliferating cells nuclear antigen (PCNA) positive dermal fibroblasts, expression of thyroid hormone receptors-α and -β in dermal fibroblasts were examined.

[Age-related changes in the content of serine-arginine protein kinase 1 (SRPK1) in human dermis.].

The aim of our work was to examine content of serine-arginine protein kinase 1 (SRPK1) in human dermis at different ages (from 20 weeks of pregnancy to 85 years old). SRPK1, proliferating cells nuclear antigen (PCNA ), endothelial marker CD31 were detected in sections of the skin by indirect immunohistochemistry. Results showed, that content of SRPK1 in dermal fibroblasts was increased form antenatal period to 20 years of life followed by a decrease until 61-85 years period.

[Age-related changes in the content of sirtuin 1 in fibroblasts of human dermis].

The goal of our work was to examine content of sirtuin 1 in human skin at different ages to uncover a role of sirtuin 1 in aging of human skin. Sirtuin 1, proliferating cells nuclear antigen (PCNA) were detected by indirect immunohistochemistry in sections of the skin of human fetuses died antenatally from 22 to 40 weeks of pregnancy and humans from birth to 85 years old, died from various causes. Our results showed that the level of sirtuin 1 in dermal fibroblasts was decreased from prenatal period to 85 years old.

[Age-related changes in the expression of lamin B receptors in fibroblasts of human dermis].

The aim of this work was to study lamin B receptors in human skin at different ages. Lamin B receptors, proliferating cells nuclear antigen (PCNA) were detected in sections of the skin by indirect immunohistochemistry. Our results showed that both portion of dermal fibroblasts with positive staining for lamin B receptors and intensity of staining of fibroblasts for lamin B receptors were maximal from birth to 20 years as compared to all other examined age-periods (from 20 weeks of pregnancy to 85 years old).

Gyromagnetic RF source for interdisciplinary research.

We demonstrate a source of high power nanosecond RF pulses based on gyromagnetic nonlinear transmission line. The source is designed to explore the exposure of different biological objects to strong RF fields in an air filled rectangular waveguide loaded onto ethanol RF load. The RF pulse amplitude can be varied by 52 dB, reaching a maximum value of nearly 40 kV/cm and decreasing to tens of V/cm.

[CHANGES OF THE CONTENT OF DLL4 AND Jag-1 ANGIOGENESIS REGULATORS IN HUMAN DERMIS IN ONTOGENESIS].

The goal of this study was to examine the contents of D114 and Jag-1 angiogenesis regulators in human dermis at different age periods. D114 and Jag-1 were demonstrated by indirect immunohistochemistry in skin sections of fetuses of 20-40 gestational weeks and in persons aged from birth to 85 years. D114 was studied in 150 skin samples of 72 females and 78 males, while Jag-1 was examined in 120 samples of 58 females and 62 males.

Lamin A and lamin-associated polypeptide 2 (LAP-2) in human skin in the process of aging.

At present time, relationships between lamins and processes leading to aging are established. Mutations of genes of lamins lead to diseases, one of them is progeria. This disease is caused by violation of splaysing of lamin A gene and accumulation the farnezylated prelamin A (progerin) in the nucleus.

[Lamin B1 and lamin B2 in human skin in the process of aging].

The aim of this work was to study B type lamins in human skin at different ages. Lamins B1 and B2 were detected in sections of the skin by indirect immunohistochemistry. There were 62,3 % of dermal fibroblasts with positive staining for lamin B1 at the period from 20 to 40 weeks of gestation.

[Age-related changes of the content of angiomatin and endostatin in human skin].

Human skin structures stained positively for angiomotin or endostatin were studied by indirect immunohistochemical method. Skin specimens from frontal surface of the lower part of the neck (from upper corner of standard autopsy skin incision) from human fetuses died antenatally from 20 to 40 weeks of pregnancy, humans who died from different causes from 1 day to 85 years of life were obtained at autopsy. Positive staining for angiomotin or endostatin in the skin was found in epidermal cells, fibroblasts, sweat and sebaceous glands, blood vessels of the dermis.

[Blood vessels in human dermis during aging].

A factor that potentially influences on skin aging is blood supply which determines global conditions for an organ or a tissue functioning, including skin. Scientific data on conditions of blood supply in the skin during aging are insufficient and contradictory. Therefore, this work was aimed to the study of age-related changes in the number of blood vessels in the human dermis.

Age-related changes in angiogenesis in human dermis.

Present research is aimed to examine the number of dermal blood vessels, vascular endothelial growth factor (VEGF), delta-like ligand 4(Dll4) and Jagged-1 (Jag-1) in dermal blood vessels of human from 20weeks of pregnancy to 85years old. Numbers and proliferative activity of dermal fibroblast-like cells were also examined. Blood vessels were viewed with immunohistochemical staining for von Willebrand factor or CD31.