Structural and functional analysis of a novel galactose-binding lectin derived from MW769776.

A freshwater green microalgal strain was isolated and the lectin was identified in it by a strong hemagglutination activity (HA) assay. Characterization of the algal strain was found to be (MW769776). A single step affinity chromatographic technique was developed to purify lectin (CSL) using guar gum as the affinity matrix.

Extracellular phytase from Aspergillus niger CFR 335: purification and characterization.

Phytase, that is extensively used as a feed additive is capable of hydrolyzing phytic acid, an antinutrient found in about 60-80 % of all the plant commodities. This enzyme improves the bioavailability of essential minerals such as Ca(2+), Mg(2+), P, Zn(2+), Fe(3+), that are bound to phytic acid. An extracellular phytase from a local fungal isolate, Aspergillus niger CFR 335 was purified to homogeneity through a three-step column chromatography using DEAE-Sephadex anion exchanger.

Effect of different cultural conditions for phytase production by Aspergillus niger CFR 335 in submerged and solid-state fermentations.

The present article deals with the studies on the effect of media ingredients, such as carbon, nitrogen, inorganic phosphates, surfactants, and metal salts, on phytase enzyme production by Aspergillus niger CFR 335 in submerged (SmF) and solid-state fermentations (SSF). The results obtained showed a 1.5-fold higher enzyme yield in the presence of sucrose in both SmF and SSF, while peptone was found to be a favorable nitrogen source for SmF.