Publications by authors named "Gunasekaran Dharanivasan"
Article Synopsis
- - Diarrheagenic E. coli infections contribute to antibiotic resistance, prompting a need for better monitoring tools that can quickly identify both the pathogen and its antibiotic resistance at the point of care (POC).
- - Researchers developed an electrochemical chip-based biosensor that detects enteropathogenic E. coli (EPEC) and its resistance to β-lactam antibiotics by using specific antibodies and a unique dual-channel setup for accurate and simultaneous analysis.
- - The biosensor employs electrochemical techniques, demonstrating effective detection limits for identifying pathogenic bacteria and their antibiotic resistance markers, potentially aiding in controlling the spread of infections.
Detection of microbial contamination in water is imperative to ensure water quality. We have developed an electrochemical method for the detection of using bi-functional magnetic nanoparticle (MNP) conjugates. The bi-functional MNP conjugates were prepared by terminal-specific conjugation of anti- IgG antibody and the electroactive marker ferrocene.
View Article and Find Full Text PDFVitagenic Effect of Specific Bioactive Fractions of Rhodiola with Trachurus sp. Extract Against Oxidative Stress-Induced Aging in Human Amnion Derived Epithelial Cell Line: In View of a Novel Senolytic.
Curr Aging Sci
December 2021
Background: Rhodiola rosea is a herb that has been used in traditional medicine for several years, and LF is a class of lipoproteins derived from the fish Trachurus sp. (LF-T), which exhibits known anti-inflammatory activity.
Objective: Investigating the anti-aging effect of Rhodiola specific bioactive fractions cluster in combination with LF-T (R-L compound) in HO mediated oxidative stress-induced human amnion derived epithelial cell line - FL cells as normal human cell line.
Scanometric detection of tomato leaf curl New Delhi viral DNA using AuNP-conjugated mono- and bifunctional oligo probes through direct DNA hybridization assay (DDH assay) and sandwich DNA hybridization assay (SDH assay) with silver enhancement was developed. Tomato leaf curl New Delhi virus (ToLCNDV) coat protein gene-specific thiol-modified ssoligo probes were used for the preparation of mono- and bifunctional AuNP-ssoligo probe conjugates (signal probes). ssDNA arrays were prepared using polymerase chain reaction (PCR), rolling circle amplification (RCA), genomic DNAs fragments, and phosphate-modified positive control/capture probes through 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide/1-methylimidazole conjugation on the amine-modified glass slide (GS) surface.
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- A research study explores the use of a plant pathogenic virus (SLCCNV) as a scaffold for creating nanotechnology applications by generating a cargo-like structure.
- The study utilizes a yeast expression system to efficiently obtain coat protein monomers from SLCCNV, which are then assembled into a nanocargo architecture capable of encapsulating magnetic nanoparticles by modifying the pH of the environment.
- Additionally, bioinformatics tools were applied to study self-assembly kinetics and bioconjugation sites, and findings indicate that the SLCCNV-nanocargo exhibits low toxicity, suggesting its potential for biomedical applications.
We demonstrated label free ethidium bromide assisted characterization of DNA interaction with cholate capped AuNPs. Interactions between ss/ds DNA and AuNPs with two different lengths (0.5 and 0.
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