cell migration enhancing constituents from , a plant used in the preparation of 'Pinda oil', a medicinal oil used in Ayurveda for wound management.

, Wight & Arn. (Apocyanaceae) is a one of the raw materials used in the preparation of 'Pinda oil', a medicinal oil which is used for treatment of wounds in Ayurveda. Of the hexanes, dichloromethane, and ethyl acetate extracts derived from the plant raw materials used to prepare 'Pinda oil', the hexanes extract of exhibited the highest mean percentage wound closure (75.

A rare phytosterol, stigmast-5-en-3,7,22-triol and other secondary metabolites from showing enhanced cell migration and proangiogenic activity.

(Burm. f.) Merr.

Reassignment of NMR spectroscopic data of oleana-9(11),12-diene-3-ol isolated from and its wound healing potential in terms of cell migration and proangiogenic activity.

(Asteraceae), a plant endemic to Sri Lanka, is used for the treatment of wounds. The scratch wound assay (SWA) guided fractionation of hexanes extract of led to the isolation of oleana-9(11),12-diene-3-ol () which showed enhanced cell migration in SWA and significant proangiogenic response in chorioallantoic membrane (CAM) assay. Since the reported H NMR assignments of were incomplete, and some C NMR assignments were inconsistent with our observations, reassignment of NMR spectroscopic data of was carried out.

cell migration enhancing and pro-angiogenic active secondary metabolites from (L.) H. Rob., S.C. Keeley & Skvarla (Asteraceae).

(L.) H. Rob.

Auto-oxidation of -beyer-15-en-19-al isolated from the essential oil of the heartwood of Roxb.: formation of 15,16-epoxy--beyeran-19-oic acid and other products.

Chemical investigation of the essential oil obtained from the heartwood of Roxb. yielded three beyerene type diterpenoids -beyer-15-ene (), -beyer-15-en-19-ol (erythroxylol A) () and -beyer-15-en-19-al (). -beyer-15-en-19-al () was found to be unstable at room temperature, giving rise to hitherto unknown 15,16-epoxy--beyeran-19-oic acid ().

Dual action antifungal small molecule modulates multidrug efflux and TOR signaling.

There is an urgent need for new strategies to treat invasive fungal infections, which are a leading cause of human mortality. Here, we establish two activities of the natural product beauvericin, which potentiates the activity of the most widely deployed class of antifungal against the leading human fungal pathogens, blocks the emergence of drug resistance, and renders antifungal-resistant pathogens responsive to treatment in mammalian infection models. Harnessing genome sequencing of beauvericin-resistant mutants, affinity purification of a biotinylated beauvericin analog, and biochemical and genetic assays reveals that beauvericin blocks multidrug efflux and inhibits the global regulator TORC1 kinase, thereby activating the protein kinase CK2 and inhibiting the molecular chaperone Hsp90.

Oxaspirol B with p97 Inhibitory Activity and Other Oxaspirols from Lecythophora sp. FL1375 and FL1031, Endolichenic Fungi Inhabiting Parmotrema tinctorum and Cladonia evansii.

A new metabolite, oxaspirol D (4), together with oxaspirols B (2) and C (3) were isolated from Lecythophora sp. FL1375, an endolichenic fungus isolated from Parmotrema tinctorum, whereas Lecythophora sp. FL1031 inhabiting the lichen Cladonia evansii afforded oxaspirols A (1), B (2), and C (3).

Functional chromatographic technique for natural product isolation.

Natural product discovery arises through a unique interplay between chromatographic purification and biological assays. Currently, most techniques used for natural product purification deliver leads without a defined biological action. We now describe a technique, referred to herein as functional chromatography, that deploys biological affinity as the matrix for compound isolation.

Citriquinones A and B, new benzoquinones from Penicillium citrinum.

Two new benzoquinones, citriquinone A (1) and B (2), were isolated from the methanol extract of a soil fungus, Penicillium citrinum. The structures of the new compounds were determined on the basis of detailed spectroscopic analysis. Citriquinone A (1) exhibited antibacterial activity against Bacillus sp.

Microbial biotransformation of 16α,17-epoxy-ent-kaurane-19-oic acid by Beauveria sulfurescens ATCC 7159-F.

Biotransformation of 16alpha,17-epoxy-ent-kaurane-19-oic acid (1) by Beauveria sulfurescens ATCC 7159-F led to the production of a new ent-kaurane diterpenoid, 7beta,17-dihydroxy-ent-kaur-15-en-19-oic acid (7), and four other ent-kauranes (8 - 11), all of which were identified as their methyl esters. Compounds 9 and 10 were found to be new stereoisomers. Structures of these were established by the extensive usage of their spectroscopic characteristics.

Structure-activity relationship (SAR) of withanolides to inhibit Hsp90 for its activity in pancreatic cancer cells.

Withaferin A (WA), a naturally occurring steroidal lactone, directly binds to Hsp90 and leads to the degradation of Hsp90 client protein. The purpose of this study is to investigate the structure activity relationship (SAR) of withanolides for their inhibition of Hsp90 and anti-proliferative activities in pancreatic cancer cells. In pancreatic cancer Panc-1 cells, withaferin A (WA) and its four analogues withanolide E (WE), 4-hydroxywithanolide E (HWE), 3-aziridinylwithaferin A (AzWA) inhibited cell proliferation with IC50 ranged from 1.

Synthesis and biological evaluation of novobiocin analogues as potential heat shock protein 90 inhibitors.

Recent studies have shown that novobiocin (NB), a member of the coumermycin (CA) family of antibiotics with demonstrated DNA gyrase inhibitory activity, inhibits Heat shock protein 90 (HSP90) by binding weakly to a putative ATP-binding site within its C-terminus. To develop more potent HSP90 inhibitors that target this site and to define structure-activity relationships (SARs) for this class of compounds, we have synthesized twenty seven 3-amido-7-noviosylcoumarin analogues starting from NB and CA. These were evaluated for evidence of HSP90 inhibition using several biological assays including inhibition of cell proliferation and cell cycle arrest, induction of the heat shock response, inhibition of luciferase-refolding in vitro, and depletion of the HSP90 client protein c-erbB-2/HER-2/neu (HER2).

Geopyxins A-E, ent-kaurane diterpenoids from endolichenic fungal strains Geopyxis aff. majalis and Geopyxis sp. AZ0066: structure-activity relationships of geopyxins and their analogues.

Four new ent-kaurane diterpenoids, geopyxins A-D (1-4), were isolated from Geopyxis aff. majalis, a fungus occurring in the lichen Pseudevernia intensa, whereas Geopyxis sp. AZ0066 inhabiting the same host afforded two new ent-kaurane diterpenoids, geopyxins E and F (5 and 6), together with 1 and 3.

Asperpyrone D and other metabolites of the plant-associated fungal strain Aspergillus tubingensis.

Bioactivity-guided fractionation of a cytotoxic extract of Aspergillus tubingensis, a fungal strain occurring in the rhizosphere of the Sonoran desert plant, Fallugia paradoxa, afforded a dimeric naphtho-gamma-pyrone asperpyrone D, nine known naphtho-gamma-pyrones, funalenone, and the cytotoxic cyclic penta-peptide, malformin A1.

Actin microfilament aggregation induced by withaferin A is mediated by annexin II.

The actin cytoskeleton supports diverse cellular processes such as endocytosis, oriented growth, adhesion and migration. The dynamic nature of the cytoskeleton, however, has made it difficult to define the roles of the many accessory molecules that modulate actin organization, especially the multifunctional adapter protein annexin II. We now report that the compound withaferin A (1) can alter cytoskeletal architecture in a previously unknown manner by covalently binding annexin II and stimulating its basal F-actin cross-linking activity.