Description of a new species of sucking louse Hoplopleura kuhnludwigi (Phthiraptera: Anoplura: Hoplopleuridae) from the Cape York rat Rattus leucopus cooktownensis (Rodentia: Muridae) in Australia using conventional methodology and novel epiflourescence microscopy.

A new species of sucking louse, Hoplopleura kuhnludwigi (Phthiraptera: Hoplopleuridae), from the Cape York rat Rattus leucopus cooktownensis Tate, 1951 (Rodentia: Muridae) is described and illustrated with photos and drawings. The host rat(s) were trapped in Northeast Queensland, Australia and identified using morphological characters and DNA sequencing. Hoplopleura kuhnludwigi is the first louse species to be described on R.

An evaluation of African animal trypanosomiasis control strategies in remote communities of Eastern Zambia.

Communities living in African animal trypanosomiasis (AAT) endemic areas of Zambia use several control strategies to protect their livestock from the devastating effects of trypanosomiasis. Several studies have reported the effectiveness of trypanosomiasis control strategies based on retrospective data. In this study, we assessed incidence rates of AAT in cattle ( = 227) using a prospective cohort study comprising 4 treatment groups, i.

A review of risk factors at the human-animal-environmental interface of garbage dumps that are driving current and emerging zoonotic diseases.

An increasing trend in zoonotic and emerging infectious diseases (EIDs) has been observed worldwide. Most EID outbreaks originate from wildlife, and these outbreaks often involve pathogen-host-environment interaction. Garbage dumps act as an interface between humans, animals, and the environment, from which EIDs could arise.

Seroprevalence of canid alphaherpesvirus-1 and associated risk factors in domestic dogs in North Queensland, Australia.

Canid alphaherpesvirus-1 (CaHV-1) may cause a highly fatal haemorrhagic disease in neonatal pups and is associated with reproductive, respiratory and ocular disease in older dogs. Although assumed to have a world-wide distribution, there have been few reports of CaHV-1 in Australia. The aim of this study was to investigate the seroprevalence of CaHV-1 in household dogs in a residential suburb in Townsville, as well as in dogs attending two dog shows in the region.

A comparison of risk factor investigation and experts' opinion elicitation analysis for identifying foot-and-mouth disease (FMD) high-risk areas within the FMD protection zone of South Africa (2007-2016).

Foot-and-mouth disease is a controlled disease in accordance with the South African Animal Diseases Act (Act 35 of 1984). The country was classified by the World Organisation for Animal Health (WOAH) as having a FMD free zone without vaccination in 1996. However, this status was suspended in 2019 due to a FMD outbreak outside the controlled zones.

Toxoplasmosis epidemic in a population of urbanised allied rock-wallabies (Petrogale assimilis) on Magnetic Island (Yunbenun), North Queensland.

A mortality event involving 23 allied rock-wallabies (Petrogale assimilis) displaying neurological signs and sudden death occurred in late April to May 2021 in a suburban residential area directly adjacent to Magnetic Island National Park, on Magnetic Island (Yunbenun), North Queensland, Australia. Three allied rock-wallabies were submitted for necropsy, and in all three cases, the cause of death was disseminated toxoplasmosis. This mortality event was unusual because only a small, localised population of native wallabies inhabiting a periurban area on a tropical island in the Great Barrier Reef World Heritage Area were affected.

The conjunctival fungal microflora of horses in a North Queensland tropical environment and their in vitro susceptibilities to antifungal agents.

Fungi are ubiquitous in the environment and part of the commensal microflora on the conjunctiva of equine eyes. North Queensland, being tropical, presents an ideal environment for fungi growth. When the cornea is injured, fungi can invade the corneal stroma, resulting in keratomycosis.

Japanese Encephalitis Enzootic and Epidemic Risks across Australia.

Japanese encephalitis virus (JEV) is an arboviral, encephalitogenic, zoonotic flavivirus characterized by its complex epidemiology whose transmission cycle involves reservoir and amplifying hosts, competent vector species and optimal environmental conditions. Although typically endemic in Asia and parts of the Pacific Islands, unprecedented outbreaks in both humans and domestic pigs in southeastern Australia emphasize the virus' expanding geographical range. To estimate areas at highest risk of JEV transmission in Australia, ecological niche models of vectors and waterbirds, a sample of piggery coordinates and feral pig population density models were combined using mathematical and geospatial mapping techniques.

Comparison of ionised calcium measured using a portable analyser to a reference method in healthy dogs.

Objectives: To compare the ionised calcium measured on a portable analyser (iSTAT, Abbott) to a reference method.

Materials And Methods: Blood samples from 39 apparently healthy dogs were analysed in duplicate using a portable analyser and a reference method (Radiometer ABL800 FLEX). Bland-Altman plots and Passing-Bablok regression were used to assess constant and proportional bias between the two instruments.

The detection of African trypanosomes in goats reared in tsetse infested villages of Eastern Zambia.

Control programmes for African animal trypanosomiasis (AAT) in livestock have been mainly focused on cattle with very little focus on goats, an important reservoir for the disease. Using the polymerase chain reaction (PCR), this study investigated trypanosome infection in village goats in Mambwe, a rural District in Eastern Zambia. Filter paper blood spots were collected from 326 goats and tested for infection with Trypanosoma congolense, Trypanosoma vivax and Trypanosoma brucei s.

A review of pig and poultry diseases in the Eastern Cape Province of South Africa, 2000-2020.

The informal poultry and pig sector in the Eastern Cape Province (ECP) of South Africa is of significant socio-economic importance as it sustains livelihoods and ensures food security; yet little is known about the distribution and prevalence of infectious and zoonotic diseases in this region. This paper reviews data published for pig and poultry diseases in the province during the last 20 years (2000-2020). The review included relevant published papers identified by a computerised literature search from Web of Science; provincial animal health reports; the national database from the Department of Agriculture, Land Reform and Rural Development (DALRRD); animal health reports submitted by DALRRD to the World Organisation for Animal Health (OIE) via the World Animal Health Information Database (WAHID) interface and laboratory records.

Using value chain and trade networks in the Eastern Cape Province of South Africa, as a basis for targeted rural chicken surveillance.

Despite the benefits of rural chickens in the Eastern Cape Province (ECP) of South Africa, this sector is still underdeveloped and poorly surveyed for poultry diseases. The lack of a sustainable poultry disease surveillance system coupled with communities and practices where the interactions between birds are high, emphasize the need for targeted surveillance of chicken diseases in the province. However, to set up such a system requires knowledge of the value chain and trade networks.

Prevalence of trypanosomes and selected symbionts in tsetse species of eastern Zambia.

Insect symbionts have attracted attention for their potential use as anti-parasitic gene products in arthropod disease vectors. While tsetse species of the Luangwa valley have been extensively studied, less is known about the prevalence of symbionts and their interactions with the trypanosome parasite. Polymerase chain reaction was used to investigate the presence of and bacteria, in tsetse flies infected with trypanosomes ( and ).

Policy and Linkages in the Application of a One Health System for Reporting and Controlling African Trypanosomiasis and Other Zoonotic Diseases in Zambia.

The capacity to detect, control and manage emerging and re-emerging zoonotic diseases in Africa has been limited by a lack of utilisation of available reporting structures and policies to support programmes at national and local levels. This study explored the impact of the Zambian government policies on animal and human disease reporting and management and on One Health opportunities. An in-depth review and analysis of strengths, weaknesses, opportunities, and threats in the existing policies and reporting structures in the departments responsible for Veterinary Services, Health, and Wildlife, was conducted.

Spatial distribution of foot-and-mouth disease (FMD) outbreaks in South Africa (2005-2016).

Foot-and-mouth disease (FMD) is a transboundary animal disease that has negative socioeconomic consequences including impacts on food security. In South Africa, FMD outbreaks in communal farming communities cause major livestock and human livelihood concerns; they raise apprehensions about the effectiveness of FMD control measures within the FMD protection areas. This study aimed to identify high-risk areas for FMD outbreaks at the human/domestic animal/wildlife interface of South Africa.

Challenges in the Diagnostic Performance of Parasitological and Molecular Tests in the Surveillance of African Trypanosomiasis in Eastern Zambia.

African animal trypanosomiasis (AAT) control programs rely on active case detection through the screening of animals reared in disease endemic areas. This study compared the application of the polymerase chain reaction (PCR) and microscopy in the detection of trypanosomes in cattle blood in Mambwe, a rural district in eastern Zambia Blood samples were collected from 227 cattle and tested for infection with trypanosomes using microscopy and Ribosomal RNA Internal Transcribed Spacers (ITS)-PCR. Microscopy on the buffy coat detected 17 cases, whilst thin and thick smears detected 26 cases and 28 cases, respectively.

Susceptibility of boar spermatozoa to heat stress using in vivo and in vitro experimental models.

Induction of heat stress as an experimental procedure in animals is commonly used to examine heat-related impacts on sperm quality. This study aimed to develop potential heat stress models that could be used at any time of the year, to advance the study of seasonal infertility in the pig under controlled conditions. Heat stress was induced by either housing boars (n = 6) at 30 °C inside a hot room for 42 days (55-65% humidity; LD 12:12 h; in vivo), or by heating boar semen (n = 7) for 30 min at various temperatures (35.

Insights into the Control and Management of Human and Bovine African Trypanosomiasis in Zambia between 2009 and 2019-A Review.

Tsetse transmitted trypanosomiasis is a fatal disease commonly known as in cattle and sleeping sickness in humans. The disease threatens food security and has severe economic impact in Africa including most parts of Zambia. The level of effectiveness of commonly used African trypanosomiasis control methods has been reported in several studies.

A Serological Survey of Selected Papua New Guinea Blood Donors for Hepatitis B and Related Co-Infections.

Hepatitis B virus (HBV) infection is a serious problem and earlier studies in Papua New Guinea have reported a high prevalence of hepatitis B virus infection. These studies were undertaken using insensitive tests and before an expanded immunization program. The current HBV status is therefore uncertain.

A novel method for performing antigenic vaccine matching for foot-and-mouth disease in absence of the homologous virus.

Foot-and-mouth-disease (FMD) is a highly contagious transboundary animal disease that has negative consequences on regional and international trade. Vaccination is an important approach for FMD control and an essential consideration is the degree of cross-protection conferred by the vaccine against currently circulating field viruses. The objective of this study was to evaluate a new vaccine matching technique that does not require knowledge concerning the homologous vaccine virus.

Antioxidant supplementation mitigates DNA damage in boar (Sus scrofa domesticus) spermatozoa induced by tropical summer.

Heat stress-induced sperm DNA damage has recently been demonstrated in boars during tropical summer; which could negatively impact early embryo survival and litter size in sows. Given the boar's inefficient capacity to sweat, non-pendulous scrotum and low antioxidant activity in seminal plasma, elevated endogenous levels of antioxidants are needed to combat reactive oxygen species induced during periods of heat stress. This should prevent the build-up of pathological levels of DNA damage in boar spermatozoa.

Patient and tumour factors influencing canine mast cell tumour histological grade and mitotic index.

The aim of this study was to identify patient and tumour factors most frequently associated with high histological grades of canine mast cell tumours (MCTs). Search criteria in a shared database of multiple Animal Referral Hospital locations within Australia generated 400 canine MCTs in 286 patients. Patient and tumour data were extrapolated and the association between a tumour being histologically high grade and patient and tumour factors, including: patient breed, patient gender and neuter status, patient age at MCT excision, tumour location and tumour size was assessed using univariate analysis.