Rapid and facile detection of Mycoplasma pneumoniae 16S rRNA based on CHA-FICA system.

Mycoplasma pneumoniae (MP) is a major a rapid, sensitive and reliable method for the detection of 16S ribosomal RNA (16S rRNA) was developed based on catalytic hairpin assembly (CHA) technology and digoxine-biotin double-labeled fluorescence immunochromatography (FICA) strip. The signal amplification achieved by CHA avoids the dependence on expensive thermal cycling equipment. The method can obtain the results within 1 h in the absence of an enzyme, and the minimum detection limit can reach 1 pM.