Qualitative comparison of decalcifiers for mouse bone cryosections for subsequent biophotonic analysis.

Bone tissue, with its complex structure, often necessitates decalcification of the hard tissue for ex vivo morphological studies. The choice of a suitable decalcification method plays a crucial role in preserving desired features and ensuring compatibility with diverse imaging techniques. The search for a universal decalcification method that is suitable for a range of biophotonic analyses remains an ongoing challenge.

Quantification of Polystyrene Uptake by Different Cell Lines Using Fluorescence Microscopy and Label-Free Visualization of Intracellular Polystyrene Particles by Raman Microspectroscopic Imaging.

Environmental pollution caused by plastic is a present problem. Polystyrene is a widely used packaging material (e.g.

Insights into -Induced Bone Deformation in a Mouse Model of Chronic Osteomyelitis Using Fluorescence and Raman Imaging.

Osteomyelitis is an infection of the bone that is often difficult to treat and causes a significant healthcare burden. is the most common pathogen causing osteomyelitis. Osteomyelitis mouse models have been established to gain further insights into the pathogenesis and host response.

High-throughput Raman spectroscopy allows characterization of murine small intestinal intra-epithelial lymphocytes (IEL).

T cells are considered to be critical drivers of intestinal inflammation in mice and people. The so called intra-epithelial lymphocyte (IEL) compartment largely consist of T cells. Interestingly, the specific regulation and contribution of IELs in the context of inflammatory bowel disease remains poorly understood, in part due to the lack of appropriate analysis tools.

Label-Free Characterization of Macrophage Polarization Using Raman Spectroscopy.

Macrophages are important cells of the innate immune system that play many different roles in host defense, a fact that is reflected by their polarization into many distinct subtypes. Depending on their function and phenotype, macrophages can be grossly classified into classically activated macrophages (pro-inflammatory M1 cells), alternatively activated macrophages (anti-inflammatory M2 cells), and non-activated cells (resting M0 cells). A fast, label-free and non-destructive characterization of macrophage phenotypes could be of importance for studying the contribution of the various subtypes to numerous pathologies.

Discrimination of Strains from Coagulase-Negative Staphylococci and Other Pathogens by Fourier Transform Infrared Spectroscopy.

is an extremely infectious and malignant pathogen among many bacteria species. The aim of this work is to provide a robust classification model that would be able to identify independent of the culture growth stage and the variations in bacteria concentration in suspension and also one that would be able to identify the pathogen among both taxonomically close species of the same genus and taxonomically distant species of different genera, using Fourier transform infrared spectroscopy (FTIR). In total, the spectra of 141 isolates of 17 bacteria have been used.

[Decision on the rational algorithm in treatment of kidney cysts].

The article presents an algorithm of diagnostics and treatment of renal cysts and other liquid neoplasms of the retroperitoneal space on an analysis of 270 case histories. The algorithm takes into account the achievements of modern medical technologies developed in the recent years. The application of the proposed algorithm must elevate efficiency of the diagnosis and quality of treatment of patients with renal cysts.

[Mechanism of alcohol action on gastric secretion].

In chronic experiment on dogs (3 dogs with Pavlov's miniature stomach, 3 dogs with Heidenhain's miniature stomach) the mechanism of action of alcohol (8%-150 ml) on gastric secretion was clarified. For this purpose the new inhibitor of gastric secretion--glycopeptide was utilized, which action was preset in laboratory of G.K.

[Current possibilities of diagnostic and surgical endoscopy in cicatricial esophageal stenosis].

Endoscopic methods of examination have been employed in 153 cases of burn and peptic strictures of the esophagus. The experience has shown that the use of small-diameter (including ultrathin) fiber endoscopes provides maximum information of the status of the esophagus along its whole length as well as of the stomach and duodenum, which is of importance in the choice of therapeutic tactics in this category of patients. Eighty one patients with cicatricial stenosis of the esophagus and esophageal anastomoses were subjected to various endoscopic interventions: balloon hydrodilation, bougienage, electrocision, and endoprosthesis .

[Endoscopic study using a superthin endoscope in benign esophageal strictures].

To increase the diagnostic possibilities, the authors suggested a method of endoscopic examination of patients with esophageal strictures by means of a superthin PF-28 fibroscope produced by the Olympus firm (Japan), which was introduced along a string-guide. The examination was successful in 24 of 26 patients. Clinical observations have shown that esophagogastroscopy with a superthin endoscope makes it possible to recognize the exact length of the stenosed segment, detect the second zone of stenosis, and determine the condition of the mucous membrane of the esophago-gastric junction and the proximal part of the stomach.