Evaluation of 3M™ loop-mediated isothermal amplification-based kit and 3M™ ready-to-use plating system for detection of in naturally contaminated leafy vegetables, chicken, and their related processing environments.

The effectiveness of two different rapid methods involving the 3M™ molecular detection assay and the 3M™ Petrifilm environmental Plate were evaluated for the rapid detection of from naturally contaminated vegetables and chicken-processing environments against the standard culture-based method. A total of 178 samples were examined for the presence of A total of 47/178 (26.4%) by standard ISO culture-based method (EN ISO 11290-1), 42/178 (23.

Antibiotic susceptibility and molecular characterization of Salmonella enterica serovar Paratyphi B isolated from vegetables and processing environment in Malaysia.

Salmonella enterica serovar Paratyphi B (S. Paratyphi B) is a major foodborne pathogen distributed all over the world. However, little is known about the antibiotic resistance, genetic relatedness and virulence profile of S.

Data on antibiogram and resistance genes harboured by strains and their Pulsed-field gel electrophoresis clusters.

This article describes the Pulsed-field gel electrophoresis clustering of the predominant strains ( ser. Albany, ser. Brancaster, and ser.

Genetic relatedness, antimicrobial resistance and biofilm formation of Salmonella isolated from naturally contaminated poultry and their processing environment in northern Malaysia.

We investigated the genetic relatedness, antibiotic resistance and biofilm-producing ability of 114 strains of Salmonella, belonged to three serotypes (Corvallis, Brancaster and Albany), isolated from naturally contaminated poultry and their environment in wet markets and smale-scale processing plant from northern Malaysia. Pulsed-field gel electrophoresis revealed that Salmonella strains isolated from various wet markets were clonally related, suggesting the widespread dissemination of these three serotypes in northern Malaysia. All except one strain of Salmonella were resistant to more than two classes of antibiotics, hence regarded as multidrug resistant (MDR).

Floricoccus tropicus gen. nov., sp. nov. and Floricoccus penangensis sp. nov. isolated from fresh flowers of durian tree and hibiscus.

Three strains of Gram-staining-positive, coccus-shaped, lactic acid bacteria, designated as HibF3, HibF2 and HibF5 were isolated from fresh flowers of hibiscus, and a fourth, DF1, was isolated from fresh flowers of durian tree, in Penang, Malaysia. Taxonomic characterisation was performed by polyphasic analysis. Sequence similarities of the 16S rRNA gene and the housekeeping rpoA and pheS genes of these strains with their closely-related lactococcal and streptococcal relatives were 92-94, 78 and 81 %, respectively.

Prevalence of in poultry processing environments in wet markets in Penang and Perlis, Malaysia.

Aim: The aim of this study was to determine the prevalence of various serotypes in chickens, carcass contact surfaces as well as environmental samples collected from wet markets and small scale processing plant.

Materials And Methods: A total of 182 poultry and environmental samples were collected at random on separate occasions from wet markets and small scale processing plant, during the period of October 2014 to July 2015 in Penang and Perlis, Malaysia. The samples were analyzed for the presence of using ISO 6579:2002 conventional culture-based method.

Genome Sequence of "Anthococcus," a Novel Genus of the Family Streptococcaceae Isolated from Flowers.

Here, we report the draft whole-genome sequence of "Anthococcus," a novel genus of the family Streptococcaceae isolated from fresh flowers of a durian (Durio zibethinus) tree. The draft genome of Anthococcus sp. strain DF1 contains 2,157,756 bp, with a G+C content of 33.

Physio-chemical, microbiological properties of tempoyak and molecular characterisation of lactic acid bacteria isolated from tempoyak.

This study aims to determine physio-chemical properties of tempoyak, characterise the various indigenous species of lactic acid bacteria (LAB) present at different stages of fermentation and also to determine the survival of selected foodborne pathogens in tempoyak. The predominant microorganisms present in tempoyak were LAB (8.88-10.

Genetic Relatedness of Salmonella Serovars Isolated from Catfish (Clarias gariepinus) and Tilapia (Tilapia mossambica) Obtained from Wet Markets and Ponds in Penang, Malaysia.

A total of 43 Salmonella enterica isolates belonging to different serovars (Salmonella Albany, Salmonella Agona, Salmonella Corvallis, Salmonella Stanley, Salmonella Typhimurium, Salmonella Mikawasima, and Salmonella Bovismorbificans) were isolated from catfish (Clarias gariepinus) and tilapia (Tilapia mossambica) obtained from nine wet markets and eight ponds in Penang, Malaysia. Thirteen, 19, and 11 isolates were isolated from 9 of 32 catfish, 14 of 32 tilapia, and 11 of 44 water samples, respectively. Fish reared in ponds were fed chicken offal, spoiled eggs, and commercial fish feed.

Antibiotic Application and Emergence of Multiple Antibiotic Resistance (MAR) in Global Catfish Aquaculture.

Catfish is one of the most cultivated species worldwide. Antibiotics are usually used in catfish farming as therapeutic and prophylactic agents. In the USA, only oxytetracycline, a combination of sulfadimethoxine and ormetoprim, and florfenicol are approved by the Food Drug Administration for specific fish species (e.

Composition, physicochemical properties and thermal inactivation kinetics of polyphenol oxidase and peroxidase from coconut (Cocos nucifera) water obtained from immature, mature and overly-mature coconut.

Composition, physicochemical properties and enzyme inactivation kinetics of coconut water were compared between immature (IMC), mature (MC) and overly-mature coconuts (OMC). Among the samples studied, pH, turbidity and mineral contents for OMC water was the highest, whereas water volume, titratable acidity, total soluble solids and total phenolics content for OMC water were the lowest. Maturity was found to affect sugar contents.

Growth properties and cholesterol removal ability of electroporated Lactobacillus acidophilus BT 1088.

This study aimed to evaluate the effects of electroporation on the cell growth, cholesterol removal, and adherence abilities of L. acidophilus BT 1088 and their subsequent passages. The growth of electroporated parent cells increased (P<0.

Prevalence, antibiotic resistance and RAPD typing of Campylobacter species isolated from ducks, their rearing and processing environments in Penang, Malaysia.

We report for the first time on the prevalence, antibiotic resistance and RAPD types of Campylobacter species in ducks and duck related environmental samples in Malaysia. Samples were examined by enrichment in Bolton Broth followed by plating onto modified Charcoal Cefoperazone Deoxycholate agar (mCCDA) and/or plating directly onto mCCDA. A total of 643 samples were screened, and the prevalence of Campylobacter spp.

Ultrasound treatment enhances cholesterol removal ability of lactobacilli.

This study aimed to evaluate the effect of ultrasound treatment on the cholesterol removing ability of lactobacilli. Viability of lactobacilli cells was significantly increased (P < 0.05) immediately after treatment, but higher intensity of 100 W and longer duration of 3 min was detrimental on cellular viability (P < 0.

Hemolytic and nonhemolytic vancomycin-resistant Enterococcus faecalis isolated from beef imported to Malaysia.

Twenty-two strains of vancomycin-resistant Enterococcus faecalis were isolated from 9 (6%) of 150 samples of frozen beef and beef products imported to Malaysia. The isolates were obtained from eight samples of beef and one sample of minced beef patty. No E.

Molecular characterization of Vibrio cholerae O1 outbreak strains in Miri, Sarawak (Malaysia).

Bacterial resistance to various antimicrobial agents is common in area with high usage of antibiotics. In this study, the data on antimicrobial susceptibility patterns of Vibrio cholerae O1 from patients during an outbreak period was found to be high but variable rates of multidrug resistance. Thirty-two of 33 V.

Microbiology of Chili Bo, a Popular Malaysian Food Ingredient.

The predominant microbial flora of a specific Malaysian food ingredient, chili bo (containing 9% ground dried chilies, 0.6% acetic acid, and 5 to 10% cornstarch, wt/vol) stored for up to 25 days at 28°C without added benzoic acid (product A) and with 7,000 ppm of added benzoic acid (product B) was examined. Aerobic plate counts for both products were initially 6.

Survival and Growth of Vibrio cholerae O139 in Selected Malaysian Street Foods.

The growth of Vibrio cholerae O139 inoculated into (a mixture of coconut milk, brown sugar, and green jelly from rice flour), (prawn paste, sugar, soy sauce, spices, garlic, and peanut gravy), gravy, tofu, fried tofu, and wheat-flour noodles (all except gravy containing the natural microbial flora) was examined at four incubation temperatures (7, 15,25, and 35°C). V. cholerae O139 grew well in incubated at 25 and 35°C but not at 15°C or below.

Growth and Aflatoxin Production by Aspergillus parasiticus NRRL 2999 in the Presence of Acetic or Propionic Acid and at Different Initial pH Values.

Experiments were done to determine effects of different concentrations of acetic or propionic acid in a glucose-yeast extract-salts medium with an initial pH value of 4,5 or 5.5 on growth and aflatoxin production by Aspergillus parasiticus NRRL 2999. Amounts of aflatoxin were measured with reversed-phase high-performance liquid chromatography.

Growth and Aflatoxin Production by Aspergillus parasiticus NRRL 2999 in the Presence of Lactic Acid and at Different Initial pH values.

Twenty-five milliliters of glucose-yeast-salts medium containing 0, 0.5, 0.75, 1.

Growth and Aflatoxin Production by Aspergillus parasiticus NRRL 2999 in the Presence of Potassium Benzoate or Potassium Sorbate and at Different Initial pH Values.

Experiments were done to determine how different concentrations of potassium benzoate or potassium sorbate in a glucose-yeast extract-salts medium with an initial pH value of 3.5, 4.5 or 5.

Growth of and Aflatoxin Production by Aspergillus parasiticus in a Medium Containing Potassium Chloride or a Mixture of Potassium Chloride and Sodium Chloride.

Twenty-five milliliters of glucose-yeast-salts medium containing 0, 2, 4, 6, 8 and 10% KCl or a mixture of NaCl (%) and KCl (%) (0:0, 1.5:0.5, 3.

Growth and Aflatoxin Production by Aspergillus parasiticus in the Presence of Sodium Chloride.

Twenty-five milliliters of glucose-yeast-salt medium containing 0, 2, 4, 6, 8, or 10% NaCl was inoculated to contain, approximately 10 or 10 conidia of Aspergillus parasiticus NRRL 2999 and then incubated at 13 or 28°C. Amounts of aflatoxin produced were determined using Reversed-Phase High Performance Liquid Chromatography (HPLC). Increasing the concentration of NaCl reduced accumulation of aflatoxin and also induced a lag in growth of the culture.